Development and Characterization of Gelatin-Based Bio-Nanocomposite Films Reinforced with Carbon Dots for Enhanced Mechanical and Optical Properties
DOI:
https://doi.org/10.15408/jkv.v12i1.48995Keywords:
Bio-nanocomposites, carbon dots , films , gelatinAbstract
Gelatin-based bio-nanocomposite films reinforced with carbon dots were developed and characterized to evaluate their mechanical, thermal, and optical properties. The films were prepared with carbon dot concentrations of 0.0, 0.1, 0.5, and 1.0% (w/w) to examine the effect of nanofiller incorporation. Optical analysis showed enhanced ultraviolet–visible absorption at 339–344 nm and red-shifted fluorescence emission at 500–517 nm under 400 nm excitation, indicating improved light-responsive behavior. The addition of carbon dots significantly improved tensile strength, elongation at break, and Young’s modulus, demonstrating enhanced flexibility and mechanical performance. Furthermore, carbon dots reduced the water vapor transmission rate and transparency while increasing film density, indicating improved barrier properties. The film containing 0.5% carbon dots showed the most balanced performance, with a tensile strength of 27.43 ± 0.71 MPa, elongation at break of 23.17 ± 1.66%, and Young’s modulus of 0.26 ± 0.03 GPa. Structural analysis confirmed no significant changes in chemical composition and showed uniform dispersion of carbon dots. Thermal analysis indicated improved stability with a melting temperature of 167°C. These findings highlight the potential of gelatin-based bio-nanocomposite films for sustainable packaging applications.
Downloads
References
1. Nurilmala M, Darmawan N, Putri EAW, Jacoeb AM, Irawadi TT. Pangasius Fish Skin and Swim Bladder as Gelatin Sources for Hard Capsule Material. Int J Biomater. 2021;2021:1-6. doi:10.1155/2021/6658002
2. Kandra R, Bajpai S. Synthesis, mechanical properties of fluorescent carbon dots loaded nanocomposites chitosan film for wound healing and drug delivery. Arab J Chem. 2020;13:4882-4894. doi:10.1016/j.arabjc.2019.12.010
3. Abedinia A, Alimohammadi F, Teymori F, Razgardani N, Asl MRS, Ariffin F, Nafchi AM, Huda N, Roslan J. Characterization and cell viability of probiotic/prebiotics film based on duck feet gelatin: A novel poultry gelatin as a suitable matrix for probiotics. Foods. 2021;10(8):1-16. doi:10.3390/foods10081761
4. Amjadi S, Emaminia S, Heyat Davudian S, Pourmohammad S, Hamishehkar H, Roufegarinejad L. Preparation and characterization of gelatin-based nanocomposite containing chitosan nanofiber and ZnO nanoparticles. Carbohydr Polym. 2019;216:376-384. doi:10.1016/j.carbpol.2019.03.062
5. Kundu S, Das A, Basu A, Abdullah MF, Mukherjee A. Guar gum benzoate nanoparticle reinforced gelatin films for enhanced thermal insulation, mechanical and antimicrobial properties. Carbohydr Polym. 2017;170:89-98. doi:10.1016/j.carbpol.2017.04.056
6. Nurilmala M, Suryamarevita H, Husein Hizbullah H, Jacoeb AM, Ochiai Y. Fish skin as a biomaterial for halal collagen and gelatin. Saudi J Biol Sci. 2021;(xxxx):1-11. doi:10.1016/j.sjbs.2021.09.056
7. Shankar S, Teng X, Li G, Rhim JW. Preparation, characterization, and antimicrobial activity of gelatin/ZnO nanocomposite films. Food Hydrocoll. 2015;45:264-271. doi:10.1016/j.foodhyd.2014.12.001
8. Hosseini SF, Rezaei M, Zandi M, Farahmandghavi F. Fabrication of bio-nanocomposite films based on fish gelatin reinforced with chitosan nanoparticles. Food Hydrocoll. 2015;44:172-182. doi:10.1016/j.foodhyd.2014.09.004
9. Sarfraz J, Gulin-Sarfraz T, Nilsen-Nygaard J, Pettersen MK. Nanocomposites for food packaging applications: An overview. Nanomaterials. 2021;11(10):1-27. doi:10.3390/nano11010010
10. Khusairy M, Rahman MR, Taib SNL, Khui PLN, Kakar A, Jayamani E. Nano-Reinforcement in Sustainable Polymer Composites. Woodhead Publishing; 2021. doi:10.1016/b978-0-12-820338-5.00010-2
11. Roy S, Rhim JW. Gelatin-based film integrated with copper sulfide nanoparticles for active packaging applications. Appl Sci. 2021;11(14):1-12. doi:10.3390/app11146307
12. Arfat YA, Ahmed J, Hiremath N, Auras R, Joseph A. Thermo-mechanical, rheological, structural and antimicrobial properties of bionanocomposite films based on fish skin gelatin and silver-copper nanoparticles. Food Hydrocoll. 2017;62:191-202. doi:10.1016/j.foodhyd.2016.08.009
13. Wang H, Zhang M, Ma Y, Wang B, Huang H, Liu Y, Shao M, Kang Z, Huang H, Liu Y, Kang Z. Carbon Dots Derived from Citric Acid and Glutathione as a Highly Efficient Intracellular Reactive Oxygen Species Scavenger for Alleviating the Lipopolysaccharide-Induced Inflammation in Macrophages. ACS Appl Mater Interfaces. 2020;12(37):41088-41095. doi:10.1021/acsami.0c11735
14. Zhou Y, Sharma SK, Peng Z, Leblanc RM. Polymers in carbon dots: A review. Polymers (Basel). 2017;9(2):67. doi:10.3390/polym9020067
15. Sreenath PR, Mandal S, Panigrahi H, Das P, Dinesh Kumar K. Carbon dots: Fluorescence active, covalently conjugated and strong reinforcing nanofiller for polymer latex. Nano-Structures and Nano-Objects. 2020;23:100477. doi:10.1016/j.nanoso.2020.100477
16. Dai Y, Wang J, Tao P, He R. Various hydrophilic carbon dots doped high temperature proton exchange composite membranes based on polyvinylpyrrolidone and polyethersulfone. J Colloid Interface Sci. 2019;553:503-511. doi:10.1016/j.jcis.2019.06.020
17. Yuan Z, Wu X, Jiang Y, Li Y, Huang J, Hao L, Zhang J, Wang J. Carbon dots-incorporated composite membrane towards enhanced organic solvent nanofiltration performance. J Memb Sci. 2018;549:1-11. doi:10.1016/j.memsci.2017.11.051
18. Dong Y, Wang R, Li H, Shao J, Chi Y, Lin X, Chen G. Polyamine-functionalized carbon quantum dots for chemical sensing. Carbon N Y. 2012;50(8):2810-2815. doi:10.1016/j.carbon.2012.02.046
19. Zhu S, Meng Q, Wang L, Zhang J, Song Y, Jin H, Zhang K, Sun H, Wang H, Yang B. Highly Photoluminescent Carbon Dots for Multicolor Patterning, Sensors, and Bioimaging. Angew Chemie. 2013;125(14):4045-4049. doi:10.1002/ange.201300519
20. Kasprzyk W, Świergosz T, Bednarz S, Walas K, Bashmakova N V., Bogdał D. Luminescence phenomena of carbon dots derived from citric acid and urea-a molecular insight. Nanoscale. 2018;10(29):13889-13894. doi:10.1039/c8nr03602k
21. Wang Y, Li Y, Xu Y. Synthesis of mechanical responsive carbon dots with fluorescence enhancement. J Colloid Interface Sci. 2020;560:85-90. doi:10.1016/j.jcis.2019.10.039
22. Long C, Jiang Z, Shangguan J, Qing T, Zhang P, Feng B. Applications of carbon dots in environmental pollution control: A review. Chem Eng J. 2021;406(2021):126848. doi:10.1016/j.cej.2020.126848
23. Wu B, Zhu G, Dufresne A, Lin N. Fluorescent Aerogels Based on Chemical Crosslinking between Nanocellulose and Carbon Dots for Optical Sensor. ACS Appl Mater Interfaces. 2019;11(17):16048-16058. doi:10.1021/acsami.9b02754
24. Syafei D, Sugiarti S, Darmawan N, Khotib M. Synthesis of TiO2/carbon nanoparticle (C-dot) composites as active catalysts for photodegradation of persistent organic pollutant. Indones J Chem. 2017;17(1):37-42. doi:10.22146/ijc.23615
25. Anindita F, Darmawan N, Mas’Ud ZA. Fluorescence carbon dots from durian as an eco-friendly inhibitor for copper corrosion. AIP Conf Proc. 2018;(02008):1-7. doi:10.1063/1.5054412
26. Zhai X, Zou X, Shi J, Huang X, Sun Z, Li Z, Sun Y, Li Y, Wang X, Holmes M, Gong Y, Povey M, Xiao J. Amine-responsive bilayer films with improved illumination stability and electrochemical writing property for visual monitoring of meat spoilage. Sensors Actuators, B Chem. 2020;302(July 2019):127130. doi:10.1016/j.snb.2019.127130
27. Bhattacharyya SK, Dule M, Paul R, Dash J, Anas M, Mandal TK, Das P, Das NC, Banerjee S. Carbon Dot Cross-Linked Gelatin Nanocomposite Hydrogel for pH-Sensing and pH-Responsive Drug Delivery. ACS Biomater Sci Eng. 2020;6(10):5662-5674. doi:10.1021/acsbiomaterials.0c00982
28. Campalani C, Causin V, Selva M, Perosa A. Fish-Waste-Derived Gelatin and Carbon Dots for Biobased UV-Blocking Films. ACS Appl Mater Interfaces. 2022;14(30):35148-35156. doi:10.1021/acsami.2c11749
29. Sendão R, Yuso M del VM de, Algarra M, Esteves da Silva JCG, Pinto da Silva L. Comparative life cycle assessment of bottom-up synthesis routes for carbon dots derived from citric acid and urea. J Clean Prod. 2020;254:1-10. doi:10.1016/j.jclepro.2020.120080
30. Sugiarti S, Darmawan N. Synthesis of fluorescence carbon nanoparticles from ascorbic acid. Indones J Chem. 2015;15(2):141-145. doi:10.22146/ijc.21207
31. Geleta TT, Habtegebreil SA, Tolesa GN. Physical, mechanical, and optical properties of enset starch from bulla films influenced by different glycerol concentrations and temperatures. J Food Process Preserv. 2020;44(8):1-9. doi:10.1111/jfpp.14586
32. Pellá MCG, Silva OA, Pellá MG, Beneton AG, Caetano J, Simoes MR, Dragunski DC. Effect of gelatin and casein additions on starch edible biodegradable films for fruit surface coating. Food Chem. 2020;309:125764. doi:10.1016/j.foodchem.2019.125764
33. ISO 1183-1. Plastics-Methods for Determining the Density of Non-Cellular Plastics. ISO; 2019.
34. Akhavan-Kharazian N, Izadi-Vasafi H. Preparation and characterization of chitosan/gelatin/nanocrystalline cellulose/calcium peroxide films for potential wound dressing applications. Int J Biol Macromol. 2019;133:881-891. doi:10.1016/j.ijbiomac.2019.04.159
35. ASTM D882-12. Standard Test Method for Tensile Properties of Thin Plastic Sheeting. American Society for Testing and Materials; 2012.
36. Zhang X, Xu H, Li Y, Xu Y. Carbon-Dot-Based Thin Film with Responses toward Mechanical Stimulation and Acidic/Basic Vapors. ACS Omega. 2020;5(21):12144-12147. doi:10.1021/acsomega.0c00465
37. Yang Z, Chaieb S, Hemar Y. Gelatin-Based Nanocomposites: A Review. Polym Rev. 2021;61(4):765-813. doi:10.1080/15583724.2021.1897995
38. Procopio FR, Lourenço RV, Ana M, Bitante QB, Jose P, Ant M, Jachinto C. Sustainable Biopolymer Films from Amazonian Tambatinga Fish Waste : Gelatin Extraction and Performance for Food Packaging Applications. foods. 2025;14(3866):1-18.
39. Kavoosi G, Dadfar SMM, Dadfar SMA, Ahmadi F, Niakosari M. Investigation of gelatin/multi-walled carbon nanotube nanocomposite films as packaging materials. Food Sci Nutr. 2014;2(1):65-73. doi:10.1002/fsn3.81
40. Japanesse Industrial Standard (JIS). Japanesse Industrial Standard. 2 : 1707. Japanese Standards Association; 1975.
41. Shanmugam K, Doosthosseini H, Varanasi S, Garnier G, Batchelor W. Nanocellulose films as air and water vapour barriers: A recyclable and biodegradable alternative to polyolefin packaging. Sustain Mater Technol. 2019;22:e00115. doi:10.1016/j.susmat.2019.e00115
42. Khoirunnisa AR, Joni IM, Panatarani C, Rochima E, Praseptiangga D. UV-screening, transparency and water barrier properties of semi refined iota carrageenan packaging film incorporated with ZnO nanoparticles. AIP Conf Proc. 2018;1927(030041):1-7. doi:10.1063/1.5021234
43. Atarés L, Chiralt A. Essential oils as additives in biodegradable films and coatings for active food packaging. Trends Food Sci Technol. 2016;48:51-62. doi:10.1016/j.tifs.2015.12.001
44. Andrade MA, Barbosa CH, Ribeiro-santos R, Tome S, Fernando AL, Silva AS, Vilarinho F. Emerging Trends in Active Packaging for Food : A Six-Year Review. Foods. 2025;14(2713):1-43.
45. Liu J, Li R, Yang B. Carbon Dots: A New Type of Carbon-Based Nanomaterial with Wide Applications. ACS Cent Sci. 2020;6(12):2179-2195. doi:10.1021/acscentsci.0c01306
46. Kan J, Li M, Liu M, Jiang N, Yue Z, Yu H, Sun R. Interfacial Engineering of High-Performance Pickering Emulsion – Gelatin Composite Films for Active Packaging. Foods. 2025;14(3978):1-26.
47. Nuvoli L, Conte P, Fadda C, Ruiz R, Garcia M, Baldino S, Mannu A. Structural , thermal , and mechanical properties of gelatin-based films integrated with tara gum. Polymer (Guildf). Published online 2020:1-9. doi:10.1016/j.polymer.2020.123244
48. Zhou E, Xi J, Guo Y, Liu Y, Xu Z, Peng L, Gao W, Ying J, Chen Z, Gao C. Synergistic effect of graphene and carbon nanotube for high-performance electromagnetic interference shielding films. Carbon N Y. 2018;133:316-322. doi:10.1016/j.carbon.2018.03.023
49. Ilyas RA, Sapuan SM, Ibrahim R, Abrar H, Ishak MR, Zainudin ES, Atikah MSN, Mohd Nurazzi N, Atiqah A, Ansari MSN, Syafri E, Asrofi M, Sari NH, Jumaidin R. Effect of sugar palm nanofibrillated cellulose concentrations on morphological, mechanical and physical properties of biodegradable films based on agro-waste sugar palm (Arenga pinnata (Wurmb.) Merr) starch. J Mater Res Technol. 2019;8(5):4819-4830. doi:10.1016/j.jmrt.2019.08.028
50. Boateng J, Khan S. Composite HPMC-Gelatin Films Loaded with Cameroonian and Manuka Honeys Show Antibacterial and Functional Wound Dressing Properties. gels. 2025;11(557):1-23.
51. Shrungi M, Goswami A, Bajpai J, Bajpai AK. Designing kaolin-reinforced bionanocomposites of poly(vinyl alcohol)/gelatin and study of their mechanical and water vapor transmission behavior. Polym Bull. 2019;76(11):5791-5811. doi:10.1007/s00289-019-02684-4
52. Haghighi H, De Leo R, Bedin E, Pfeifer F, Siesler HW, Pulvirenti A. Comparative analysis of blend and bilayer films based on chitosan and gelatin enriched with LAE (lauroyl arginate ethyl) with antimicrobial activity for food packaging applications. Food Packag Shelf Life. 2019;19(November 2018):31-39. doi:10.1016/j.fpsl.2018.11.015
53. Meindrawan B, Suyatma NE, Wardana AA, Pamela VY. Nanocomposite coating based on carrageenan and ZnO nanoparticles to maintain the storage quality of mango. Food Packag Shelf Life. 2018;18(November 2017):140-146. doi:10.1016/j.fpsl.2018.10.006
54. Binaymotlagh R, Chronopoulou L, Palocci C. An Overview of Biopolymer-Based Graphene Nanocomposites for Biotechnological Applications. Materials (Basel). 2025;18(2978):1-35.
55. Shankar S, Wang LF, Rhim JW. Effect of melanin nanoparticles on the mechanical, water vapor barrier, and antioxidant properties of gelatin-based films for food packaging application. Food Packag Shelf Life. 2019;21(January):100363. doi:10.1016/j.fpsl.2019.100363
56. Ahammed S, Liu F, Khin MN, Yokoyama WH, Zhong F. Improvement of the water resistance and ductility of gelatin film by zein. Food Hydrocoll. 2020;105:105804. doi:10.1016/j.foodhyd.2020.105804
57. Nilsuwan K, Benjakul S, Prodpran T. Properties and antioxidative activity of fish gelatin-based film incorporated with epigallocatechin gallate. Food Hydrocoll. 2018;80:212-221. doi:10.1016/j.foodhyd.2018.01.033
58. Wu X, Tong Z, Liu Y, Yang Y, Li Y, Cheng Y, Yu J, Liu N. Cationic carbon dots : A novel class of mimetic enzymes. Nano Res. 2025;18(94907333):1-23.
59. Cazón P, Velazquez G, Ramírez JA, Vázquez M. Polysaccharide-based films and coatings for food packaging: A review. Food Hydrocoll. 2017;68:136-148. doi:10.1016/j.foodhyd.2016.09.009
60. Sentanin L, Gonçalves J, Filho DO, Egea MB, Henrique L, Mattoso C. Smart and Mechanically Enhanced Zein – Gelatin Films Incorporating Cellulose Nanocrystals and Alizarin for Fish Spoilage Monitoring. Foods. 2025;14(3015):1-18.
61. American Society for Testing and Materials. ASTM. Standard test methods for tensile properties of thin plastic sheeting, method D882-10. Annu B ASTM Stand. 2010;87(Reapproved):3-5. doi:10.1520/D0882-10.2
62. Loo CPY, Sarbon NM. Chicken skin gelatin films with tapioca starch. Food Biosci. 2020;35(100589):1-8. doi:10.1016/j.fbio.2020.100589
63. Bang Y, Shankar S, Rhim J. In situ synthesis of multi-functional gelatin / resorcinol / silver nanoparticles composite films. Food Packag Shelf Life. 2019;22(July):100399. doi:10.1016/j.fpsl.2019.100399
64. Nagarajan M, Benjakul S, Prodpran T, Songtipya P. Characteristics of bio-nanocomposite films from tilapia skin gelatin incorporated with hydrophilic and hydrophobic nanoclays. J Food Eng. 2014;143:195-204. doi:10.1016/j.jfoodeng.2014.06.038
65. Sahraee S, Ghanbarzadeh B, Milani JM, Hamishehkar H. Development of Gelatin Bionanocomposite Films Containing Chitin and ZnO Nanoparticles. Food Bioprocess Technol. 2017;10(8):1441-1453. doi:10.1007/s11947-017-1907-2
66. Leta TB, Adeyemi JO, Fawole OA. Carbon Dot Nanoparticles Synthesized from Horticultural Extracts for Postharvest Shelf-Life Extension of Fruits and Vegetables. Plants. 2025;14(2523):1-51.
67. León-lópez A, Flores-gutiérrez EV, Cenobio-galindo ADJ, Islas-moreno A. Gelatin-Based Films Containing Extracts of Prickly Pear ( Opuntia guerrana ): Characterization and Evaluation of Bioactive Properties. Foods. 2025;14(3911):1-16.
68. Augusto V, Rodrigues GDM, Monteiro VM, Carvalho RAD, Silva C, Maria C, Yoshida P, Martelli SM, Velasco JI, Fakhouri FM. A Gelatin-Based Film with Acerola Pulp : Production , Characterization , and Application in the Stability of Meat Products. Polymers (Basel). 2025;17(1882):1-15.
69. Sultan M, Ibrahim H, Mohammed H, Masry E, Hassan YR. Antimicrobial gelatin ‑ based films with cinnamaldehyde and ZnO nanoparticles for sustainable food packaging. Sci Rep. 2024;24(24499):1-21. doi:10.1038/s41598-024-72009-7
70. Fonseca J de M, Valencia GA, Soares LS, Dotto MER, Campos CEM, Moreira RDFPM, Fritz ARM. Hydroxypropyl methylcellulose-TiO2 and gelatin-TiO2 nanocomposite films: Physicochemical and structural properties. Int J Biol Macromol. 2020;151:944-956. doi:10.1016/j.ijbiomac.2019.11.082
71. Azizi-Lalabadi M, Alizadeh-Sani M, Divband B, Ehsani A, McClements DJ. Nanocomposite films consisting of functional nanoparticles (TiO2 and ZnO) embedded in 4A-Zeolite and mixed polymer matrices (gelatin and polyvinyl alcohol). Food Res Int. 2020;137:109716. doi:10.1016/j.foodres.2020.109716
72. Castro MCR, André MP, Pereira P, Cruz V, Machado AV, Rodrigues PV. Tailoring PLA / Gelatin Film Properties for Food Packaging Using Deep Eutectic Solvents. Molecules. 2026;31(39):1-17.
73. Rubini K, Menichetti A, Cassani MC, Montalti M, Bigi A, Boanini E. The Role of WO3 Nanoparticles on the Properties of Gelatin Films. gels. 2024;10(354):1-15.
74. Shankar S, Teng X, Rhim J. Effects of concentration of ZnO nanoparticles on mechanical, optical , thermal, and antimicrobial properties of gelatin/ZnO nanocomposite Films. Korean J Packag Sci Technol. 2014;20(2):41-49.
75. Riahi Z, Priyadarshi R, Rhim J, Bagheri R. Food Hydrocolloids Gelatin-based functional films integrated with grapefruit seed extract and TiO 2 for active food packaging applications. Food Hydrocoll. 2021;112(September 2020):106314. doi:10.1016/j.foodhyd.2020.106314
76. Bai R, Zhang X, Yong H, Wang X, Liu Y, Liu J. Development and characterization of antioxidant active packaging and intelligent Al 3+ -sensing films based on carboxymethyl chitosan and quercetin. Int J Biol Macromol. 2019;126(2019):1074-1084. doi:10.1016/j.ijbiomac.2018.12.264
77. Tavassoli M, Alizadeh M, Khezerlou A, Ehsani A, Julian D. Food Hydrocolloids Multifunctional nanocomposite active packaging materials : Immobilization of quercetin , lactoferrin , and chitosan nanofiber particles in gelatin films. Food Hydrocoll. 2021;118(March):106747. doi:10.1016/j.foodhyd.2021.106747
78. Kavoosi G, Rahmatollahi A, Mohammad Mahdi Dadfar S, Mohammadi Purfard A. Effects of essential oil on the water binding capacity, physico-mechanical properties, antioxidant and antibacterial activity of gelatin films. LWT - Food Sci Technol. 2014;57(2):556-561. doi:10.1016/j.lwt.2014.02.008
79. Kanmani P, Rhim JW. Properties and characterization of bionanocomposite films prepared with various biopolymers and ZnO nanoparticles. Carbohydr Polym. 2014;106(1):190-199. doi:10.1016/j.carbpol.2014.02.007
80. Li X, Wang W, Li Q, Lin H, Xu Y, Zhuang L. Design of Fe3O4@SiO2@mSiO2-organosilane carbon dots nanoparticles: Synthesis and fluorescence red-shift properties with concentration dependence. Mater Des. 2018;151(2017):89-101. doi:10.1016/j.matdes.2018.04.051
81. Ren J, Malfatti L, Innocenzi P. Citric Acid Derived Carbon Dots, the Challenge of Understanding the Synthesis-Structure Relationship. J Carbon Res. 2020;7(1):2. doi:10.3390/c7010002
82. He Q, Zhang Y, Cai X, Wang S. Fabrication of gelatin-TiO2 nanocomposite film and its structural, antibacterial and physical properties. Int J Biol Macromol. 2016;84:153-160. doi:10.1016/j.ijbiomac.2015.12.012
Downloads
Published
Issue
Section
License
Copyright (c) 2026 Prima Soheti, Charlena Charlena, Noviyan Darmawan, Mala Nurilmala

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

