Kandungan Fitokimia dan Bioaktivitas Ekstrak Metanol Biji Palem Putri (Veitchia merillii)

adawiah adawiah

Abstract


Palem putri (Veitchia merillii) is a kinds of Araceae plant which has been used by the community in cure myopic eyes and for woman who have pregnant. However, research on the content of secondary metabolites and bioactivity of palm putri has never been done. This study aims to determine the phytochemical content, total polyphenols and flavonoids and bioactivity include antioxidant activity DPPH free radical scavenging, antidiabetic method alpha-glucosidase enzyme inhibition and toxicity of the methods Bhrine Shrimp Lethal Test (BSLT). Palem putri contains secondary metabolites, alkaloids, flavonoids, terpenoids, polyphenols, phenolic hydroquinone and saponins. The methanol extract of palm putri seed has a total polyphenol content and total flavonoids respectively 642.8 mg gallic acid / gram sample extract and quercetin 543.3 mg / gram sample extract, has a very strong antioxidant activity with IC50 value of  0.82 ppm, the level of toxicity is very toxic with LC50 values ​​of 11.535 ppm. As well as having antidiabetic activity using the enzyme alpha-glucosidase inhibition with IC50 values ​​of 1.97 ppm is even higher than the activity of the compound quercetin as a positive control.

 

Keywords: Palem putri, bioactivity, antioxidant, antidiabetic, toxicity

 

Palem putri (Veitchia merillii) is a kinds of Araceae plant which has been used by the community in cure myopic eyes and for woman who have pregnant. However, research on the content of secondary metabolites and bioactivity of palm putri has never been done. This study aims to determine the phytochemical content, total polyphenols and flavonoids and bioactivity include antioxidant activity DPPH free radical scavenging, antidiabetic method alpha-glucosidase enzyme inhibition and toxicity of the methods Bhrine Shrimp Lethal Test (BSLT). Palem putri contains secondary metabolites, alkaloids, flavonoids, terpenoids, polyphenols, phenolic hydroquinone and saponins. The methanol extract of palm putri seed has a total polyphenol content and total flavonoids respectively 642.8 mg gallic acid / gram sample extract and quercetin 543.3 mg / gram sample extract, has a very strong antioxidant activity with IC50 value of  0.82 ppm, the level of toxicity is very toxic with LC50 values ​​of 11.535 ppm. As well as having antidiabetic activity using the enzyme alpha-glucosidase inhibition with IC50 values ​​of 1.97 ppm is even higher than the activity of the compound quercetin as a positive control.

 

Keywords: Palem putri, bioactivity, antioxidant, antidiabetic, toxicity

DOI: http://dx.doi.org/10.15408/jkv.v2i1.3076

Keywords


Bioaktivitas; palem putri; antioksidan; antidiabetes; toksisitas

References


Anderson JE, Goetz CM, Mc Laughlin JL. 1991. A Blind Comparison of Simple Bench-top Bioassay and Human Tumor Cell Cytotoxicities as Antitumor Prescrenss. Natural Product Chemistry, Elseiver, Amsterdam.

Arai Y, Watanabe S, Kimira M, Shimoi K, Mochizuki R, Kinae N. 2000. Dietary intakes of flavonols, flavones and isoflavones by japanese women and the inverse correlation between quercetin intake and plasma ldl cholesterol concentration. Journal of Nutritional. 30: 2243-2250.

Cahyadi R. 2009. Uji toksisitas akut ekstrak etanol buah pare (Momordica charantia L) terhadap larva Artemia salina Leach dengan metode brine shrimp lethality test (BST). Universitas Dipenogoro Repository. 5: 1-8.

Eghdami A, Moghaddasi MS, Sadegi Fatimah. 2011. Determination of antioxidant activity of juice and peel extract of three variety of pomegranate and clinical study. Advances in Environmental Biology. 5(8): 2282-2287. ISSN 1995-0756.

Evans Rice CA, Miller NJ, Paganga G. 1996. Structure antioxidant activity relationships of flavonoids and phenolic acids. Free Radical Biol. Med. 20, 933– 956.

Febrinda, Andi E, Astawa M, Wresdiyanti T, Yuliana Nancy Dewi. 2013. Kapasitas antioksidan dan inhibitor alfa glukosidase ekstrak umbi bawang dayak. Jurnal Teknologi dan Industri Pangan. 24(2): 161-167

Heyne K. 1987. Tumbuhan Berguna Indonesia, Jilid 1. Jakarta, Yayasan Sarana Wana Jaya.

Hsiu-Ling Tsai, Sam KC, Sue-Joan Chang. 2007. Antioxidant content and free radical scavenging ability of fresh red pummelo (citrus grandis l.) juice and freeze dried products. Journal Agriculture and Food Chemistry. 55: 2867-2872

Kim YJ, Kyung KJ, Lee JH, Chung HY. 2004. 4-4 Dihydroxybiphenyl as a new potent tyrosinase inhibitor”.J. Biol. Pharm. Bull. 28 (2) 323-327.

Kulkarni, Anand P, Policegoudra RS, Aradhya SM. 2007. Chemical composition and antioxidant activity of sapota (achras sapota l.) fruit. Journal Of Biochemistry. 31: 399-414

Maisuthisakul, Pitchaon, Pasuk, Sirikarn, Ritthiruangdejca. 2008. Relationship between antioxidant properties and chemical composition of some thai plants. Journal of Food Composition and Analysis. 21: 229–240.

Meskin MS, WR Bidlack, AJ Davies, ST Omaye. 2002. Phytochemicalsin Nutrition and Health. CRC Press, London-New York.

Mohamed EA, Siddiqui MJA, Ang LF, Sadikun A, Chan SH, Tan SC, Asmawi MZ, Yam MF. 2012. Potent α-glucosidase and α-amylase inhibitory activities of standardized 50% ethanolic extracts and sinensetin from Orthosiphon stamineus Benth as anti-diabetic mechanism. BMC Complementary and Alternative Med. 12- 176.

Prior RL, Hoang HA, Gu L, Wu X, Bacchiocca M, Howard L, Hampsch-Woodill M, Huang D, Ou B, Jacob R. 2003. Assay for hydrophilic and lipophilic antioxidant capacity (oxygen radical absorbance capacity (oracfl)) of plasma and other biological and food samples. Journal of Agricultural and Food Chemistry. 51: 3273-3279.

Rekha C, Poornima G, Manasa M, Abhipsa V, Devi JP, Kumar HTV, Kekuda TRP. 2012. Ascorbic acid, total phenol content and antioxidant activity of fresh juice of four ripe and unripe citrus fruits. Research Article. Chemical Science Transactions. 1(2): 303-310.

Rezaeizadeh A, Zuki ABZ, M Abdollahi, Goh YM, Noordin MM, Hamid M, Azmi, TI. 2011. Determination of antioxidant activity in methanolic and chloroformic extract of momordica charantia. African Journal of Biotechnology. 10(24): 4932-4940. ISSN 1684–5315.

Sugiwati S. 2005. Aktivitas antihiperglikemik dari ekstrak buah mahkota dewa (phaleria macrocarpa scheff boerl) sebagai inhibitor alfa glukosidase in vitro dan in vivo pada tikus putih. Tesis, Sekolah Pasca Sarjana Institut Pertanian Bogor.

Tadera K, Minami Y, Takamatsu K, Matsuoka T. 2006. Inhibition of α-glukosidase and α-amylase of flavonoid. J Nutr. Sci. Vitaminol. 52: 149-153.

Woo HD, Kim J. 2013. Dietary flavonoid intake and risk of stomach and colorectal cancer. World Journal of Gastroenterology. 7: 1011-1019.


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DOI: 10.15408/jkv.v2i1.3076

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