Isolation and Structural Characterization of Biflavonoids from Araucaria hunsteinii and Araucaria columnaris: Chemotaxonomic and Pharmacological Perspectives

Authors

  • Purwantiningsih Sugita Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University https://orcid.org/0000-0002-0305-8123
  • Widya Sekar Ayu Ningtias Graduate Student of Chemistry Study Program, Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University
  • Rafi Chandra Priandanda Graduate Student of Chemistry Study Program, Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University
  • Auliya Ilmiawati Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University
  • Kurniawanti Kurniawanti Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University
  • Dhea Demitri Agusta Graduate Student of Chemistry Study Program, Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University
  • Hanhan Dianhar Chemistry Study Program, Faculty of Mathematics and Natural Sciences (FMIPA), Jakarta State University
  • Dyah Utami Cahyaning Rahayu Department of Chemistry, Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Indonesia

DOI:

https://doi.org/10.15408/jkv.v11i2.46173

Keywords:

Araucaria, biflavonoid, chemotaxonomy 4′,4‴,7,7″-tetra-O-methylcupressuflavone, 7-O-methylcupressuflavone

Abstract

Biflavonoids are a distinctive class of dimeric flavonoids known for their diverse biological activities and chemotaxonomic significance. In this study, two biflavonoids were isolated from the acetone extracts of Araucaria hunsteinii twigs and Araucaria columnaris leaves collected from Bogor Botanical Garden, Indonesia. Chromatographic techniques, including Sephadex LH-20 column chromatography and preparative thin-layer chromatography, were employed for purification, followed by structural elucidation using LC-MS/MS and 1D/2D NMR spectroscopy. The compounds were identified as 4',4''',7,7''-tetra-O-methylcupressuflavone (1) and 7-O-methylcupressuflavone (2). Notably, this is the first report of 7-O-methylcupressuflavone isolated from A. columnaris leaves, providing new chemotaxonomic insights into the genus Araucaria. A literature-based pharmacological analysis revealed promising cytotoxic and α-glucosidase-inhibitory activities of the isolated compounds. These findings contribute to the phytochemical profiling and highlight the pharmaceutical potential of Araucaria-derived biflavonoids.

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Published

30-11-2025

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Jurnal Kimia VALENSI, Volume 11, No. 2, November 2025

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Jurnal Kimia VALENSI, Volume 11, No. 2, November 2025

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How to Cite

Isolation and Structural Characterization of Biflavonoids from Araucaria hunsteinii and Araucaria columnaris: Chemotaxonomic and Pharmacological Perspectives. (2025). Jurnal Kimia Valensi, 11(2), 250-259. https://doi.org/10.15408/jkv.v11i2.46173