β-Sitosterol Compound from Dichloromethane Extracts of Kalanchoe tomentosa (Crassulacea) Leaves and Inhibition of α-amilase Activity
DOI:
https://doi.org/10.15408/jkv.v7i1.18443Keywords:
α-amylase, β-sitosterol, Crassulaceae, enzyme, Kalanchoe tomentosaAbstract
Kalanchoe tomentosa is one of the plants of the Crassulaceae tribe that can lower blood sugar and contains compounds of terpenoids, flavonoids, alkaloids, saponins, tannins, fatty acids, steroids, and triterpenoids. This study aims to isolate and identify chemical compounds from dichloromethane extract of Kalanchoe tomentosa leaves, as well as to test the inhibitory activity of the α-amylase enzyme. Extraction was carried out by maceration using dichloromethane as a solvent, then dichloromethane extract was purified using column chromatography, the pure isolate was obtained in the form of white powder, and an inhibition test was carried out against the α-amylase enzyme. The thin layer chromatography data of pure isolates compared to pure β-sitosterol are similar. Based on the research data, it can be concluded that the chemical structure of the pure isolate is β-sitosterol, the methylene chloride extract of K. tomentosa leaves has an inhibitory activity against the α-amylase enzyme with an inhibition value of 65%. This value is greater than the positive control of acarbose which only has 37% inhibition and β-sitosterol compound by 6.7%. This value is smaller when compared to the control of acarbose at the same concentration which obtained 5% inhibition.
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Aisyah LS, Yun YF, Herlina T, Julaeha E, Zainuddin A, Nurfarida I, Hidayat AT, Supratman U, Shiono Y. 2017. Flavonoid compounds from the leaves of Kalanchoe prolifera and their cytotoxic activity against P-388 murine leukimia cells. Natural Product Sciences. 23(2): 139-145.
Andayani TM, Ibrahim MI, Asdie AH. 2009. Pengaruh kombinasi terapi sulfonilurea, metformin, dan acarbose pada pasien diabetes mellitus tipe 2. Majalah Farmasi Indonesia. 20(4): 224 – 230.
Bhutkar MA, Bhise SB. 2012. In vitro assay of alpha amylase inhibitory activity of some indigenous plants. Int. J. Chem. Sci. 10(1): 457-462.
Fuwa H. 1954. A new method for microdetermination of amylase activity by the use of amylose as the substrate. Journal of Biological Chemistry. 41(5): 583-603.
Howard W. 2015. Pengujian Aktivitas Enzim α-Amilase. Bandung (ID): ITB.
[IDF] International Diabetes Federation. 2013. IDF Diabetes
Kementrian Kesehatan RI. Badan Penelitian dan Pengembangan. 2014. Jakarta (ID): Riskesdas.
Kim YM, Jeong YK, Wang MH, Lee WY, Rhee HI. 2005. inhibitory eff ect of pine extract on alpha glucosidase activity and postprandial hyperglycemia. Nutrition. 21: 756-61.
Kim JS, Ju JB, Choi CW, Kim SC. 2006. Hypoglycemic and antihyperlipidemic effect of korean medicinal plants in alloxan induced diabetic rats. Am J of Biochemistry and Biotecnology. 2(4): 154 -160.
Li Y, Wen S, Prasad BK, Peng G, Qian GL, Yamahara J, Roufogalis BD. 2005. Punica granatum flower extract, a poten α-Glucosidase inhibitor, improves postprandial hyperglikemia in zuker diabetic fatty rats. Etnopharm. 2005 (99): 239-244.
Saleh MM, Ghoneim MM, Kottb S, El-Hela AA. 2014. Biologically active secondary metabolites from Kalanchoe tomentosa. Journal of Biomedical and Pharmaceutical Research. 3(6): 136-140.
Saifudin A. 2014, Senyawa Alam Metabolit Sekunder: Teori, Konsep, dan Teknik Pemurnian, Edisi 1., Deepublish, Yogyakarta
Widodo Y. 2014. Pemantauan penderita diabetes. Jurnal Ilmiah Kedokteran. 3(2): 55-69
Yun YF. 2015, Metabolit Sekunder dari Beberapa Tumbuhan Kalanchoe yang Tumbuh di Indonesia dan Aktivitas Sitotoksiknya terhadap Sel Murin Leukimia P-388, Disertasi, Departemen Kimia, Universitas Padjajaran, Bandung.
