Chromium Bioremediation Using a Combination of Two Indigenous Rhizobacteria In Vitro

Wahyu Surakusumah; Nurul Ilma Apriliani; Diah Kusumawaty; Wijanarka Wijanarka; Achmad Dinoto; Restu Utari Dewina

doi:10.15408/kauniyah.v19i2.44529

Authors

Wahyu Surakusumah Universitas Pendidikan Indonesia https://orcid.org/0000-0001-8057-7990
Nurul Ilma Apriliani Universitas Pendidikan Indonesia
Diah Kusumawaty Universitas Pendidikan Indonesia https://orcid.org/0000-0002-3065-2574
Wijanarka Wijanarka Universitas Dipenogoro https://orcid.org/0000-0002-1455-8166
Achmad Dinoto Badan Riset dan Inovasi Nasional (BRIN) https://orcid.org/0000-0002-9182-7665
Restu Utari Dewina Universitas Pendidikan Indonesia https://orcid.org/0009-0007-3218-5846

DOI:

https://doi.org/10.15408/kauniyah.v19i2.44529

Keywords:

Bioremediation, Chromium, Consortia, Rhizobacteria, Tanning

Abstract

Sukaregang, known for its leathercraft industry, faces chromium pollution from poorly managed tannery waste, affecting soil and water resources. The dynamic soil area influenced by plant roots, known as the rhizosphere, becomes a habitat for various microorganisms with unique metabolic capabilities that have significant potential as bioremediation agents for heavy-metal-contaminated environments. This study aims to evaluate the potential of indigenous bacterial consortia from the chromium-contaminated rhizosphere as bioremediation agents. Five bacterial genera: Pseudomonas, Citrobacter, Bacillus, Azotobacter, and Micrococcus, were identified as chromium-resistant in vitro. Compatibility tests showed that these bacterial combinations exhibited synergies without growth inhibition. Among the five consortia combinations tested (AB, BC, CD, DE, EA), consortium DE (Azotobacter and Micrococcus), exhibiting the most stable growth dynamics, characterized by a sustained stationary phase, suggesting a superior adaptive response to chromium-induced stress. Under chromium stress, the consortium EA (Micrococcus and Pseudomonas) achieved the highest chromium removal percentage at 30.78%. This study suggests that indigenous rhizobacterial consortia could be a practical approach to remediate chromium pollution in areas like Sukaregang.

Author Biography

Achmad Dinoto, Badan Riset dan Inovasi Nasional (BRIN)

Research Center for Applied Microbiology

References

Aravindhan, R., Sreeram, K. J., Rao, J. R., Nair, B. U. (2007). Biological removal of carcinogenic chromium(VI) using mixed Pseudomonas strains. The Journal of general and applied microbiology, 53(2), 71–79. doi: https://doi.org/10.2323/jgam.53.71

Azubuike, C. C., Chikere, C. B., Okpokwasili, G. C. (2016). Bioremediation techniques–classification based on site of application: principles, advantages, limitations and prospects. World Journal of Microbiology and Biotechnology, 32(11). doi: https://doi.org/10.1007/s11274-016-2137-x

Cappuccino, J., Welsh, C. (2020). Microbiology: A Laboratory Manual, 12th Edition. San Fransisco: Pearson Education, Inc.

Denaya, S., Yulianti, R., Pambudi, A., Effendi, Y. (2021). Novel microbial consortium formulation as plant growth promoting bacteria (PGPB) agent. IOP Conf. Series: Earth and Environmental Science, 637, 012030. doi: https://doi.org/10.1088/1755-1315/637/1/012030

Ed-har, A., Widyastuti, R., Djajakirana, K. (2017). Isolasi Dan Identifikasi Mikroba Tanah Pendegradasi Selulosa Dan Pektin Dari Rhizosfer Aquilaria malaccensis. Buletin Tanah dan Lahan, 1(1), 58-64.

Ellenberg. H, Dumbois. M. Dieter. (2016). Ekologi Vegetasi: Tujuan dan Metode. Jakarta: LIPI Press & Yayasan Pustaka Obor Indonesia (Alih bahasa oleh Kuswata Kartawinata dan Rochadi Abdulhadi).

George, S. E., Costenbader, C. J., Melton, T. (1985). Diauxic growth in Azotobacter vinelandii. Journal of bacteriology, 164(2), 866–871. doi: https://doi.org/10.1128/jb.164.2.866-871.1985

Gong, D., Ye, F., Pang, C., Lu, Z., Shang, C. (2020). Isolation and Characterization of Pseudomonas sp. Cr13 and Its Application in Removal of Heavy Metal Chromium. Current microbiology, 77(11), 3661–3670. doi: https://doi.org/10.1007/s00284-020-02162-5

Hagiri, M., Fukuhara, S., Kimura, Y., Manaka, A. (2024). Quantitative determination of Hexavalent chromium using a microtiter plate: Analytical performance, operational efficiency, and fixation of a colorimetric reagent in the plate wells. Microchemical Journal, 199: 110004. doi: https://doi.org/10.1016/j.microc.2024.110004

Holt, J., Sneath, P., Krieg, N. (1994). Bergey's Manual of Determinative Bacteriology 9th. Edition. USA: Williams and Wilkins Baltimore.

Kamaludeen, S. P. B., Megharaj, M., Juhasz, A. L., Sethunathan, N., Naidu, R. (2003). Chromium-Microorganism Interactions in Soils: Remediation Implications. Reviews of Environmental Contamination and Toxicology, 178, 93–164. doi: https://doi.org/10.1007/0-387-21728-2_4

Karuppiah, P., Rajaram, S. (2011). Exploring the Potential of Chromium Reducing Bacillus sp. and there Plant Growth Promoting Activities. Journal of Microbiology Research, 1(1), 17-23. doi: https://doi.org/10.5923/j.microbiology.20110101.04

Kothari, V., Patadia, M., Trivedi, N. (2011). Microwave sterilized media supports better microbial growth than autoclaved media. Research in Biotechnology, 2(5): 63-72.

Krishnamoorthy, G., Sadulla, S., Sehgal, P. K., Mandal, A. B. (2013). Greener approach to leather tanning process: d-Lysine aldehyde as novel tanning agent for chrome-free tanning. Journal of Cleaner Production, 42, 277–286. doi: https://10.1016/j.jclepro.2012.11.004

Leung, H. M., Yue, P. Y. K., Sze, S. C. W., Au, C. K., Cheung, K. C., Chan, K. L., … Li, W. C. (2019). The potential of Mikania micrantha (Chinese creeper) to hyperaccumulate heavy metals in soil contaminated by electronic waste. Environmental Science and Pollution Research. doi: https://doi.org/10.1007/s11356-019-06771-x

Meitiniarti, V. I., Nugroho, R. A., & Krave, A. S. (2014). Ragam Bakteri Pereduksi Krom dari Air Limbah Penyamakan Kulit dan Rhizosfer Acalypha indica. Prosiding Seminar Nasional Mikrobiologi. 59-63.

Munawaroh, H. S. H., Gumilar, G. G., Kartikasari, S., Kusumawaty, D. (2017). Microbial Reduction of Cr (VI) into Cr (III) by Locally Isolated Pseudomonas Aeruginosa. IOP Conference Series: Materials Science and Engineering, 180, 012279. doi: https://doi.org/10.1088/1757-899x/180/1/012279

Oliveira, H. (2012). Chromium as an Environmental Pollutant: Insights on Induced Plant Toxicity. Journal of Botany, 2012, 1–8. doi: https://doi.org/10.1155/2012/375843

Peng, Q., Chen, L., Zhou, S., Li, H., Long, J., Yao, F., Zhuang, Y., Zhang, Z., Huang, Y., & Duan, K. (2020). Co-existence of Citrobacter freundii exacerbated Pseudomonas aeruginosa infection in vivo. International journal of medical microbiology: IJMM, 310(1), 151379. doi: https://doi.org/10.1016/j.ijmm.2019.151379

Prabhakaran, P., Ashraf, M. A., Aqma, W. S. (2016). Microbial stress response to heavy metals in the environment. RSC Advances, 6(111), 109862–109877. doi: https://doi.org/10.1039/c6ra10966g

Prasad, S., Yadav, K. K., Kumar, S., Gupta, N., Cabral-Pinto, M. M. S., Rezania, S., … Alam, J. (2021). Chromium contamination and effect on environmental health and its remediation: A sustainable approaches. Journal of Environmental Management, 285, 112174. doi: https://doi.org/10.1016/j.jenvman.2021.112174

Proboningrum, A., Hadiwiyono, Widono, S., Sholahuddin. (2019). Effectivity and compatibility of Azotobacter and Bacillus for biological control agents of fusarium wilt on banana seedlings. IOP Conference Series: Earth and Environmental Science, 250, 012003. doi: https://doi.org/10.1088/1755-1315/250/1/012003

Rahayu, N. M. (2017). Uji Kemampuan Bakteri Azotobacter Dalam Proses Penyisihan Logam Kromium Pada Tanah Tercemar Kromium. (Skripsi). Fakultas Teknik Sipil dan Perencanaan Institut Teknologi Sepuluh Nopember, Surabaya.

Ramanlal, D. B., Kumar, R. N., Kumar, N., Thakkar, R. (2020). Assessing potential of weeds (Acalypha indica and Amaranthus viridis) in phytoremediating soil contaminated with heavy metals-rich effluent. SN Applied Sciences, 2(6). doi: https://doi.org/10.1007/s42452-020-2859-0

Rolfe, M. D., Rice, C. J., Lucchini, S., Pin, C., Thompson, A., Cameron, A. D., Alston, M., Stringer, M. F., Betts, R. P., Baranyi, J., Peck, M. W., Hinton, J. C. (2012). Lag phase is a distinct growth phase that prepares bacteria for exponential growth and involves transient metal accumulation. Journal of bacteriology, 194(3), 686–701. doi: https://doi.org/10.1128/JB.06112-11

Sari, D. P. (2022). Potensi Kultur Isolat Bakteri dan Fungi sebagai Agen Bioremediasi Logam Krom Secara In Vitro. (Skripsi). Fakultas Pendidikan Matematika dan Ilmu Pengetahuan Alam, Universitas Pendidikan Indonesia, Bandung.

Su, C., Li, L., Yang, Z., Chai, L., Liao, Q., Shi, Y., Li, J. (2019). Cr(VI) reduction in chromium-contaminated soil by indigenous microorganisms under aerobic condition. Transactions of Nonferrous Metals Society of China, 29(6), 1304–1311. doi: https://doi.org/10.1016/s1003-6326(19)65037-5

Subhalaksmi, A., Rajagopal, K., Nithiyanandhan, G., Devi, J., Karthikeyan, S. (2013). Chromium Phytoaccumulation by Althernanthera sessilis in vitro - A First Report. Int. J. Pharm. Bio. Sci, 4(3), 618-624.

Suparno, O., Covington, A., Evans, C. S. (2008). Teknologi Baru Penyamakan Kulit Ramah Lingkungan: Penyamakan Kombinasi Menggunakan Penyamak Nabati, Naftol, dan Oksazolidin. J. Tek. Ind. Pert., 18(2), 79-84.

Tumolo, M., Ancona, V., De Paola, D., Losacco, D., Campanale, C., Massarelli, C., Uricchio, V. F. (2020). Chromium Pollution in European Water, Sources, Health Risk, and Remediation Strategies: An Overview. Int. J. Environ. Res. Public Health, 17, 5438.

Yoshinaga, M., Ninomiya, H., Al Hossain, M. M. A., Sudo, M., Akhand, A. A., Ahsan, N., … Kato, M. (2018). A comprehensive study including monitoring, assessment of health effects and development of a remediation method for chromium pollution. Chemosphere, 201, 667–675. doi: https://10.1016/j.chemosphere.2018.03.026

Zhang, T., & Zhang, H. (2022). Microbial Consortia Are Needed to Degrade Soil Pollutants. Microorganisms, 10(2), 261. doi: http://dx.doi.org/10.3390/microorganisms10020261