The Effectiveness Assay of Healing Incutions of Nanostructured Lipid Carriers (NLCS) Ethanol Extract of Moringa Leaf (Moringa oleifera) on White Rats (Rattus norvegicus)
DOI:
https://doi.org/10.15408/kauniyah.v19i2.43051Keywords:
Formulation NLCs, Topical, Wound healingAbstract
Wounds are disruptions of tissue integrity that impair normal physiological function. Nanostructured Lipid Carriers (NLCs) are lipid-based nanosystems that enhance the delivery of bioactive compounds. Moringa oleifera leaves contain bioactive constituents, including isothiocyanates and cytokinins (zeatin), which exhibit antibacterial activity and promote cell proliferation, potentially accelerating wound healing. This study aimed to evaluate the wound-healing effectiveness of NLCs containing Moringa oleifera leaf ethanol extract. An acute open-incision wound model was established in rats, divided into five groups: normal control, betadine ointment (positive control), NLC base (negative control), extract gel, and NLCs containing 5% Moringa oleifera leaf ethanol extract. Treatments were applied topically, and wound healing was monitored by measuring wound area reduction with ImageJ. The percentage of wound closure and area under the curve (AUC) values were calculated and analyzed using one-way ANOVA. Both the extract gel and NLC formulations accelerated wound healing compared with the positive and negative controls. Complete wound closure was achieved on day 8 in the treatment groups, whereas the positive and negative controls reached complete closure on days 10 and 14, respectively. These findings indicate that NLCs containing Moringa oleifera leaf ethanol extract have potential as an effective topical formulation for promoting wound healing.
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