Histological Structure of Rat Uterine After Treatment with Nanochitosan Preparation of Ethanolic Neem Leaves Extract

Abstract

Neem leaves (Azadirachta indica A. Juss) contain compounds that have antifertility potential. The constraint in giving medicine orally is the low bioavailability and distribution of active compounds in herbal plants. This problem can be solved by packing herbal plant extracts in nanochitosan. The aim of this study was to analyze the effect of treating with a nanochitosan ethanol extract of neem leaves on the histological structure of the white rat uterine. This study was conducted for 8 months used a randomized design (CRD), which was divided into 3 treatment groups with 4 repetitions. Treatments is given for 21 days, including P0 (an aquades 2 mL/animal/day), P1 (an ethanol extract of neem leaves 2 mL/animal/day, and P2 (a nanochitosan preparation of neem leaves 2 mL/head/day. The variables measured were endometrial thickness, number of uterine glands, uterine diameter, and uterine weight. Numeric datas were analyzed statistically parametrically using the ANOVA test at a confidence level of 95%. The results showed that the endometrial thickness, uterine diameter, number of uterine glands, and uterine weight of P0 were normal, and did not significantly different (P >0.05) from P1 and P2. The conclusion of this study is that the treatment of nanochitosan ethanol extract of neem leaves has the same potential with ethanol extract of neem in affecting the histological structure of white rats uterine.