Decolorization of Reactive Black 5 by Myceliophthora thermophila KLUM1
Abstract
This research aimed to ascertain the impact of alkaline lignin of teak sawdust on the synthesis of ligninase and decolorization of Reactive Black 5 (RB5) by M. thermophilia KLUM1, also to elucidate the mechanism of mycoremediation. This research was conducted within the cultivation of the spore suspension in a co-culture setting. The RB5 dye percentage decolorization and ligninase activity were produced by varying the addition of alkaline lignin. The results show that the addition of 0.5% alkaline lignin accelerates the production of ligninase in the Kirk medium. However, adding more than 0.5% alkaline lignin does not affect the highest amount of ligninase produced. The increase in RB5 dye decolorization by M. thermophila KLUM1 is proportional to the increase in alkaline lignin in the medium. The percentage of wood-rot fungi decolorization in Kirk medium with and without lignin with the addition of 0.5%, 1%, and 2% lignin on the 10th day was 26.29%, 29.51%, 34.74%, and 40.43%, respectively. The decolorization process of RB5 by M. thermophilia KLUM1 in Kirk medium with alkaline lignin occurs through several mechanisms, i.e., enzymatic degradation, adsorption by the growing mycelia, and alkaline lignin present in the medium.
Keywords
References
Zafar S, Bukhari DA, Rehman A. Azo dyes degradation by microorganisms – An efficient and sustainable approach. Saudi J Biol Sci. 2022;29(12):103437. doi:10.1016/j.sjbs.2022.103437
Astiyandani I, Susanti E. Pemanfaatan Kapang Pelapuk Kayu ( KPK ) Indigenous Mycelioptora thermophilia KLUM1 Untuk Pengolahan Limbah Rumput Taman Sebagai Pakan Ternak. J Ilmu Hayat. 2021;5(2):58-70.
Shi Y, Yang Z, Xing L, Zhang X, Li X, Zhang D. Recent advances in the biodegradation of azo dyes. World J Microbiol Biotechnol. 2021;37(8):1-18. doi:10.1007/s11274-021-03110-6
Dimawarnita F, Syarif AM, Faramitha Y, et al. Dekolorisasi pewarna tekstil menggunakan teknik batch dan rotary biological contactor dengan tiga jenis agen hayati decolorization of textile dyes using batch and rotary biological contactor techniques with three types of biological agents. J Agroindustrial Technol. 2022;32(3):295-304.
Delila L, Susanti E, Sanjaya EH. Isolation and Screening of Indigenous Fungus Producing Lignin Peroxidase from the Cocoa Plantation in Sepawon Kediri Regency Indonesia. KnE Life Sci. 2017;3(5):131. doi:10.18502/kls.v3i5.985
Sánchez-Corzo LD, Álvarez-Gutiérrez PE, Meza-Gordillo R, Villalobos-Maldonado JJ, Enciso-Pinto S, Enciso-Sáenz S. Lignocellulolytic enzyme production from wood rot fungi collected in chiapas, mexico, and their growth on lignocellulosic material. J Fungi. 2021;7(6):1-15. doi:10.3390/jof7060450
Nihayah U. Skrining Pewarna Sintetis dan Pengaruh Aerasi Terhadap dekolorisasi pewarna oleh Kapang pelapuk Kayu isolat KLUM2. Skripsi. Published online 2018.
Eichlerová I, Baldrian P. Ligninolytic enzyme production and decolorization capacity of synthetic dyes by saprotrophic white rot, brown rot, and litter decomposing basidiomycetes. J Fungi. 2020;6(4):1-22. doi:10.3390/jof6040301
Mutmainah S, Susanti E. Ligninase Profiling and Optimization of Laccase Production from Indigenous Wood Rot Fungus (WRF) KLUM2 in Kirk Medium-Alkali Lignin Kayu Jati (MK-ALKJ). J Kim Val. 2021;7(1):75-82. doi:10.15408/jkv.v7i1.20895
Suryadi H, Judono JJ, Putri MR, et al. Biodelignification of lignocellulose using ligninolytic enzymes from white-rot fungi. Heliyon. 2022;8(2):e08865. doi:10.1016/j.heliyon.2022.e08865
Rahmawati T. Perbedaan Jenis Kayu Terhadap Produksi Ekstrak Kasar Lakase dari Thermothelomyces Guttulata KLUM2 dan Kecepatan Dekolorisasinya Terhadap Reactive Black 5. Skripsi. Published online 2020.
Oktavia J. Optimasi Jumlah Sel Inokulum dan waktu Inkubasi Kapang Pelapuk Kayu Indigenous isolat KLUM2 untuk dekolorisasi Reactive Black 5. skripsi. Published online 2018.
Rai N, Yadav M, Singh Yadav H. Enzymatic Characterisation of Lignin Peroxidase from Luffa aegyptiaca Fruit Juice. Am J Plant Sci. 2016;07(03):649-656. doi:10.4236/ajps.2016.73057
Arinta A. Penentuan Profil Ligninase dari Kapang Pelapuk Kayu Isolat Indigenus KLUM1, KLUM2, dan PnUM. Skripsi. Published online 2018.
Susanti E, Ardyati T, Suharjono, Aulani’am. Optimizing of lignin peroxidase production by the suspected novel strain of Phanerochaete chrysosporium ITB isolate. Int J ChemTech Res. 2016;9(11):24-33.
Subagyo TAW, Susanti E, Suharti. Uji Potensi dan Optimasi Konsentrasi Lignoselulosa Beberapa Limbah Pertanian Sebagai Sumber Karbon Dalam Produksi Enzim Lignin Peroksidase Oleh Phanerochaete chrysosporium. Pros Semin Nas Kim dan Pembelajarannya. Published online 2014:275-281.
Sun S, Liu P, Ullah M. Efficient Azo Dye Biodecolorization System Using Lignin-Co-Cultured White-Rot Fungus. J Fungi. 2023;9(1). doi:10.3390/jof9010091
Han Y, Shi L, Meng J, Yu H, Zhang X. Azo dye biodecolorization enhanced by Echinodontium taxodii cultured with lignin. PLoS One. 2014;9(10). doi:10.1371/journal.pone.0109786
Rybczyńska-Tkaczyk K, Korniłłowicz-Kowalska T. Biosorption optimization and equilibrium isotherm of industrial dye compounds in novel strains of microscopic fungi. Int J Environ Sci Technol. 2016;13(12):2837-2846. doi:10.1007/s13762-016-1111-3
Anita SH, Ardiati FC, Oktaviani M, et al. Immobilization of laccase from Trametes hirsuta EDN 082 in light expanded clay aggregate for decolorization of Remazol Brilliant Blue R dye. Bioresour Technol Reports. 2020;12:100602. doi:10.1016/j.biteb.2020.100602
Nurhayat OD, Ardiati FC, Ramadhan KP, et al. Bioprospecting three newly isolated white-rot fungi from Berbak-Sembilang National Park, Indonesia for biodecolorization of anthraquinone and azo dyes. Biodiversitas. 2022;23(2):613-623. doi:10.13057/biodiv/d230201
Jonstrup M, Kumar N, Guieysse B, Murto M, Mattiasson B. Decolorization of textile dyes by Bjerkandera sp. BOL 13 using waste biomass as carbon source. J Chem Technol Biotechnol. 2013;88(3):388-394. doi:10.1002/jctb.3852
Bayramoǧlu G, Yakup Arica M. Biosorption of benzidine based textile dyes “Direct Blue 1 and Direct Red 128” using native and heat-treated biomass of Trametes versicolor. J Hazard Mater. 2007;143(1-2):135-143. doi:10.1016/j.jhazmat.2006.09.002
Kusumo P, S Biyono, Tegar S. Isolasi Lignin dari Serbuk Grajen Kayu Jati (Tectona Grandis) dengan Metode Klasson. J Tek Media Pengemb Ilmu dan Apl Tek. 2020;19(2):130-139. doi:10.26874/jt.vol19no02.158
Batzias FA, Sidiras DK. Dye adsorption by prehydrolysed beech sawdust in batch and fixed-bed systems. Bioresour Technol. 2007;98(6):1208-1217. doi:10.1016/j.biortech.2006.05.020
Rajhans G, Sen SK, Barik A, Raut S. Ligninolytic enzyme system of white-rot fungi: A natural approach to bioremediation and detoxification of azo dyes in textile wastewater. Environ Eng Manag J. 2021;19(11):1983-2002. doi:10.30638/eemj.2020.188
Adnan LA, Sathishkumar P, Mohd Yusoff AR, Hadibarata T. Metabolites characterisation of laccase mediated Reactive Black 5 biodegradation by fast growing ascomycete fungus Trichoderma atroviride F03. Int Biodeterior Biodegrad. 2015;104:274-282. doi:10.1016/j.ibiod.2015.05.019
DOI: 10.15408/jkv.v11i1.45573
Refbacks
- There are currently no refbacks.
Copyright (c) 2025 Evi Susanti, Rizky Febriani Jati, Dwi Apriani, Suharti Suharti, Mieke Alvionita
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
