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  2. Jurnal Kimia VALENSI, Volume 10, No. 2, November 2024
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Synthesis of Antibacterial Coating Film Based on Eugenol-Allyl Eugenol Copolymer with Chitosan-Gelatin

Ngadiwiyana Ngadiwiyana, Hana Putri Dzahabiyyah, Ismiyarto Ismiyarto, Purbowatiningrum Ria Sarjono

Abstract


The development of coating film materials based on biopolymers and active antibacterial compounds has attracted attention in the food industries. Food packaging biopolymers can be increased antibacterial properties by adding compound modification of natural ingredients such as eugenol-allyl eugenol copolymer (PEAE). The aims of this study were to synthesize a coating film based on chitosan-gelatin with PEAE and test its antibacterial properties. PEAE synthesis was carried out by polymelirization reaction with the (BF3O(C2H5)2) as catalyst and characterized by FTIR, molecular weight, and solubility. Synthesis of chitosan-gelatin coating films with variations in PEAE concentration of 1.25%, 2.5%, and 3.75% and characterization includes FTIR, SEM, TS, E%, and contact angle. Antibacterial activity is carried out by the turbidimetry method. PEAE was synthesized with the results in the form of brown solids with 94.91% yield, molecular weight of 9,553.98 da, and the melting point of 95-98 °C. Chitosan-Gelatin films with the addition of PEAE produce a thin yellowish film, with a sequential decreased tensile strength, and the percentage value of extension increases with the increase in PEAE concentration. The best antibacterial activity in the film PEAE 2.5, with the percentage of inhibition of Staphylococcus aureus and Escherichia coli of 99.71% and 98.39% respectively.

Keywords


Antibacterial; chitosan-gelatin; coating film; copolymer; eugenol-allyl eugenol

References


Finina BF, Mersha AK. Nano-enabled antimicrobial thin films: design and mechanism of action. RSC Advances. 2024;14(8):5290-5308. doi:10.1039/d3ra07884a

Gharbi A, Kenné JP, Kaddachi R. Dynamic optimal control and simulation for unreliable manufacturing systems under perishable product and shelflife variability. International Journal of Production Economics. 2022;247: 108417. doi: https://doi.org/10.1016/j.ijpe.2022.108417

Khubiev OM, Egorov AR, Kirichuk AA, Khrustalev VN, Tskhovrebov AG, Kritchenkov AS. Chitosan-Based Antibacterial Films for Biomedical and Food Applications. International Journal of Molecular Sciences. 2023;24(13). doi:10.3390/ijms241310738

Mohamed SAA, El-Sakhawy M, El-Sakhawy MAM. Polysaccharides, Protein and Lipid -Based Natural Edible Films in Food Packaging: A Review. Carbohydrate Polymer. 2020;238: 116178. doi:10.1016/j.carbpol.2020.116178

Priyadarshi R, Rhim JW. Chitosan-based biodegradable functional films for food packaging applications. Innovative Food Science and Emerging Technologies. 2020;62(March):102346. doi:10.1016/j.ifset.2020.102346

Du C, Li S, Fan Y, Lu Y, Sheng J, Song Y. Preparation of gelatin-chitosan bilayer film loaded citral nanoemulsion as pH and enzyme stimuli-responsive antibacterial material for food packaging. International Journal of Biological Macromolecules. 2024;254:127620. doi: https://doi.org/10.1016/j.ijbiomac.2023.127620

Petkova P, Francesko A, Fernandes MM, et al. Sonochemical Coating of Textiles with Hybrid ZnO/Chitosan Antimicrobial Nanoparticles. ACS Applied Materials & Interfaces Journal. 2014;6(2):1164-1172. doi:10.1021/am404852d

Wang H, Li B, Ding F, Ma T. Improvement of properties of smart ink via chitin nanofiber and application as freshness indicator. Progress in Organic Coatings. 2020;149: 105921. doi: https://doi.org/10.1016/j.porgcoat.2020.105921

Wang H, Ding F, Ma L, Zhang Y. Edible films from chitosan-gelatin: Physical properties and food packaging application. Food Biosci. 2021;40: 100871. doi: https://doi.org/10.1016/j.fbio.2020.100871

Hassan M, Hussain D, Kanwal T, Xiao HM, Ghulam Musharraf S. Methods for detection and quantification of gelatin from different sources. Food Chemistry. 2024;438: 137970. doi: https://doi.org/10.1016/j.foodchem.2023.137970

Bertolo MR V, Martins VCA, Horn MM, Brenelli LB, Plepis AMG. Rheological and antioxidant properties of chitosan/gelatin-based materials functionalized by pomegranate peel extract. Carbohydrate Polymer. 2020;228:115386. doi: https://doi.org/10.1016/j.carbpol.2019.115386

Haghighi H, De Leo R, Bedin E, Pfeifer F, Siesler HW, Pulvirenti A. Comparative analysis of blend and bilayer films based on chitosan and gelatin enriched with LAE (lauroyl arginate ethyl) with antimicrobial activity for food packaging applications. Food Packaging and Shelf Life. 2019;19: 31-39. Doi :https://doi.org/10.1016/j.fpsl.2018.11.015

Moeini A, Mallardo S, Cimmino A, et al. Thermoplastic starch and bioactive chitosan sub-microparticle biocomposites: Antifungal and chemico-physical properties of the films. Carbohydrate Polymer. 2020;230:115627. doi: https://doi.org/10.1016/j.carbpol.2019.115627

Nair MS, Saxena A, Kaur C. Effect of chitosan and alginatebased coatings enriched with pomegranate peel extract to extend the postharvest quality of guava (Psidium guajava L.). Food Chemistry. 2018;240: 245-252. doi: https://doi.org/10.1016/j.foodchem.2017.07.122

Perumal AB, Huang L, Nambiar RB, He Y, Li X, Sellamuthu PS. Application of essential oils in packaging films for the preservation of fruits and vegetables: A review. Food Chemistry. 2022;375: 131810. doi: https://doi.org/10.1016/j.foodchem.2021.131810

Navikaite-Snipaitiene V, Ivanauskas L, Jakstas V, et al. Development of antioxidant food packaging materials containing eugenol for extending display life of fresh beef. Meat Science. 2018;145: 9-15. doi: https://doi.org/10.1016/j.meatsci.2018.05.015

Getnet TG, Kayama ME, Rangel EC, Duarte ICS, da Silva GF, Cruz NC. Atmospheric pressure plasma deposition of eugenol-derived film on metallic biomaterial for suppression of Escherichia coli and Staphylococcus aureus bacterial biofilm. Thin Solid Films. 2021;734: 138833. doi: https://doi.org/10.1016/j.tsf.2021.138833

Lan Q, Zhang X, Liang J, et al. Multifunctional polyeugenol-based nanoparticles with antioxidant and antibacterial properties. Particuology. 2024;87: 194-204. doi: https://doi.org/10.1016/j.partic.2023.08.012

Ngadiwiyana, Gunawan, Prasetya NBA, Kusworo TD, Susanto H. Synthesis and characterization of sulfonated poly(eugenol-co-allyleugenol) membranes for proton exchange membrane fuel cells. Heliyon. 2022;8(12): e12401. doi:10.1016/j.heliyon.2022.e12401

Silvianti F, Siswanta D, Aprilita N, Kiswandono A. Adsorption characteristic of iron onto poly[eugenol-co-(divinyl benzene)] from aqueous solution. Jurnal Natural. 2017; 17:108. doi:10.24815/jn.v17i2.8076

Karydis-Messinis A, Moschovas D, Markou M, et al. Development, physicochemical characterization and in vitro evaluation of chitosan-fish gelatin-glycerol hydrogel membranes for wound treatment applications. Carbohydrate Polymer Technologies and Applications. 2023;6. doi:10.1016/j.carpta.2023.100338

Zárate-Triviño DG, Hernández-Martínez SP, Bollain-y-Goytia-de-la-Rosa JJ, et al. Development of a novel scaffold of Chitosan, type IV collagen and integrin α3β1 as alternative scaffold for primary culture of podocytes. Applied Sciences (Switzerland). 2018;8(6). doi:10.3390/app8060930

Bonilla J, Poloni T, Lourenço R V., Sobral PJA. Antioxidant potential of eugenol and ginger essential oils with gelatin/chitosan films. Food Bioscience. 2018;23:107-114. doi:10.1016/j.fbio.2018.03.007

Utomo SB, Fujiyanti M, Lestari WP, Mulyani S. Antibacterial Activity Test of the C-4-methoxyphenylcalix-[4]-resorcinarene Compound Modified by Hexadecyltrimethylammonium-Bromide against Staphylococcus aureus and Escherichia coli Bacteria. JKPK (Jurnal Kimia dan Pendidikan Kimia). 2018;3(3):201. doi:10.20961/jkpk.v3i3.22742

Kosasi C, Lolo WA, Sedewi S. Isolasi dan uji aktivitas antibakteri dari bakteri yang berasosiasi dengan alga Turbinaria ornata (Turner) J. Agardh serta identifikasi secara biokimia. Pharmacon. Vol 8.; 2019. doi: https://doi.org/10.35799/pha.8.2019.29301

Wasitaningrum I. Uji resistensi bakteri Staphylococcus aureus dan Escherichia coli dari isolat susu sapi segar terhadap beberapa antibiotik. Bachelor’s Thesis, Faculty of Pharmacy, Universitas Muhammadiyah Surakarta, 2009.

Riski R, Sami F. Formulasi krim anti jerawat dari nanopartikel kitosan cangkang udang windu (Penaeusmonodon). Jurnal Farmasi. Vol. 3 No. 4, 2015. doi: https://doi.org/10.24252/jfuinam.v3i4.2261




DOI: 10.15408/jkv.v10i2.40944

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