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Screening and Optimization Consortium Technique of the Bacillus megatherium and Saccharomyces cerevisiae Microbial Consortium for Ethanol Detection

Nurdiani Nurdiani, Dyah Iswantini, Novik Nurhidayat, Wulan Tri Wahyuni, Agus Kartono

Abstract


Determination of alcohol content is very important in the food and beverage industry. Biosensor is an alternative to measuring ethanol content. Alcohol biosensors with a single microbe still have a narrow measurement area at ethanol concentrations, so a microbial consortium is needed to widen the range of measured ethanol concentrations. Therefore, it is necessary to screen the microbes from Bacillus sp and S. cerevisiae which have the potential to produce alcohol dehydrogenase (ADH) enzymes and optimize the consortium technique that can provide the best response to oxidation currents. In the yeast microbial screening of 14 S. cerevisiae isolates and 5 Bacillus sp isolates, it can be concluded that for the 14 yeast S. cerevisiae isolates that have the potential to produce ADH enzymes, there is one isolate with the SCRF code. For the 5 bacterial isolates of Bacillus sp that have the potential to produce ADH enzymes, all Bacillus isolates with the code Bacillus megatherium 29/9/14, Bacillus megatherium 23/6/22, Bacillus 6, Bacillus 53, and Bacillus 55. Based on the oxidation current data, Bacillus megatherium 23 /6/22 produces the highest current compared to other Bacillus isolates. The consortium technique that provides the highest current is the method of mixing 1:1 (µL) microbial suspension in an Eppendorf container. Optimization of the consortium's biofilms using the Response Surface Method was produced at 10 days of age, pH 7.5, and 75 µL of microbial suspension dripping volume.

Keywords


Alcohol biosensor; S. cerevisiae; Bacillus; consortium; biofilm

References


Bilgi, M., Ayranci, E. (2016). Biosensor application of screen–printed carbon electrodes modified with nanomaterials and conducting polymer ethanol biosensor based on alcohol dehydrogenase. Sensor and Actuators B: Chemical. 20490 (16): 31026−7.

Chakraborty, K., Saha, S. K., Raychaudhuri, U., Chakraborty, R. (2017). Vinegar production from vegetable waste : optimization of physical condition and kinetic modeling of fermentation process. Indian J Chem Technol. 24: 508–16.

Ferid, H. (2015). Biosensor Alkohol Menggunakan Bakteri Bacillus Penghasil Alkohol Dehidrogenase Dengan Metode Elektrokimia. [skripsi]. Bogor (ID): Institut Pertanian Bogor.

Hermansyah. (2018). Transcription of cell wall mannoproteins-1 gene in

saccharomyces cerevisiae mutant. Jurnal Kimia VALENSI: Jurnal Penelitian dan Pengembangan Ilmu Kimia. 4(2) :174-181

Houdt, R. V., Michiels, C. W. (2017). Role of bacterial cell surface structures in Escherichia coli biofilm formation. Res Microbiol.156:626–33 21.

Isvani, N. K., Mulyasuryani, A., Prasetyawan, S. (2015). Kinerja biosensor konduktometri berbasis (screen printed carbon electrode) SPCE-kitosan untuk deteksi diazinon, malation, klorpirifos dan profenofos. Jurnal Kimia VALENSI: Jurnal Penelitian dan Pengembangan Ilmu Kimia. 1(2): 83-90

Iswantini, D., Yulian, M., Mulijani, S., Trivadila. (2014). Inhibition kinetics of Sida rhombifolia L. extract toward xanthine oxidase by electrochemical method. Indonesian Journal of Chemistry. 14 (1): 71 – 77.

Iswantini, D., Nurhidayat, N., Trivadila, Widyatmoko, O. (2014). Activity and stability of uricase from Lactobacillus plantarum was Immobilizated on Natural Zeolite for Uric Acid Biosensor. Pakistan Journal of Biological Sciences. 17 (2):277-281.

Iswantini, D., Nurhidayat, N., Ferit, H. (2017). The alcohol dehydrogenase of Bacillus strain for measuring alcohol electrochemically. IOP Conf. Series: Earth and Environmental Science. 58:012010 https://doi.org/:10.1088/1755-1315/58/1/012010

Kuswandi, B., Irmawati, T., Hidayat, M. A., Jayus, Ahmad, M. (2014). A simple visual ethanol biosensor based on alcohol oxidase immobilized onto polyaniline film for halal verification of fermented beverage samples, Sensor. 14: 2135-2149

Madigan, M. T., Martinko, J. M., Brock, T. D. (2006). Brock biology of microorganisms. Upper Saddle River, N : Pearson Prentice Hall.

Mulyawan, R., Iswantini, D., Nurhidayat, N., Saprudin, D., Purwaningsih, H. (2022). Biosensor Performance of Phenol Analysis Using Microbial Consortium of Bacillus sp. and Pseudomonas sp. AIP Conference Proceedings this link is disabled. 2638. 050009

Nurhakim, M. A., Kusdiyantini, E., Raharjo, B. (2016). Penggunaan substrat glukosa berbagai konsentrasi sebagai sumber karbon microbial fuel cell Saccharomyces cerevisiae untuk menghasilkan energi listrik. Bioma: Berkala Ilmiah Biologi. 18(2): 131-136. ISSN: 1410-8801

Nurhidayat, N., Iswantini, D., Bestari, P. (2020). The accuracy of ethanol biosensor based with Acetobacter aceti biofilm in certifying halal food products. AIP Conference Proceedings 2243:03001. https://doi.org/10.1063/5.0004769

Othman, M. A., Sharifudin, S. A., Mansor, A., Kahar, A. A., Long, K. (2014). Coconut water vinegar: new alternative with improved procesing technique. Journal of Engineering Science and Technology. 293 – 302.

Putri, Y. W., Putra, A. E., Utama, B. I. (2018). Identifikasi dan karakteristik bakteri asam laktat yang diisolasi dari vagina wanita usia subur. Jurnal Kesehatan Andalas. 7.

Salvado, Z., Arroyo-Lopez, F. N., Guillamo, J. M., Salazar, G., Querol, A., Barrio, E. (2011). Temperature adaptation markedly determines evolution within the genus saccharomyces. Appl Environ Microbiol. 77(7): 2292–2302.

Somasekhar, R., Chinnadayyala, Kakoti, A., Santhosh, M., Goswami, P. (2014). A novel amperometric alcohol biosensor developed in a 3rd generation bioelectrode platform using peroxidase coupled ferrocene activated alcohol oxidase as biorecognition system. Biosensors and Bioelectronics. 55:120–126.

Susparini, N. T. A., Abidin, Z., Iswantini, D., Nurhidayat, N. (2019). Sensitive and stable ethanol biosensor development based on Acetobacter aceti biofilm for halal detection of food and beverages. Journal of Applied Biology & Biotechnology .7(06): 40-47.

Trinh, N. T. N., Masniyom, P., Maneesri, J. (2016). Optimization of culture conditions for Acetobacter aceti TISTR 102 in coconut water with supplementary banana juice. Int Food Res J. 23(3): 1300–1307

Zusfahair, Ningsih, D. R., Fatoni, A., Puspitarini, V. A. (2019). Urease application of black-eyed pea (Vigna unguiculata ssp unguiculata l.) for urea biosensor. Jurnal Kimia Valensi. 5(1): 115-123


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DOI: 10.15408/jkv.v9i1.31506

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