1. Home
  2. Jurnal Kimia VALENSI Volume 9, No. 1, May 2023
  3. Junitasari

Isolation and Identification of Lactic Acid Bacteria form Chinese Cabbage Waste by 16s rRNA Amplification using The Polymerase Chain Reaction (PCR) Method

Assyifa Junitasari, Tina Dewi Rosahdi, Yuni Siti Lestari

Abstract


Waste is material that is disposed of from sources of human and natural activities that do not yet have economic value. Physically, vegetable waste is perishable due to its high-water content, especially Chinese Cabbage waste. The purpose of this study was the isolation and identification of lactic acid bacteria from Chinese Cabbage waste. Isolation was carried out to obtain isolates of lactic acid bacteria from Chinese Cabbage waste. Then it was amplified using a Polymerase Chain Reaction (PCR) instrument. The results of chromosomal DNA amplification of bacterial isolates from mustard 1, mustard 2 and mustard 3 waste showed DNA bands with a size of ± 1200 bp, so it can be concluded that the process of amplification of the 16s rRNA gene fragment in mustard 1, mustard 2 and mustard 3 isolates was successful. The results of the 16s rRNA base sequence analysis showed that the mustard 1 isolate had a similarity index of 84.65% with Bacillus sp, the gene for the mustard 2 isolate had a similarity index of 84.09% with the Uncultured bacterium clone, and the mustard 3 isolate gene had a similarity index of 85.42% with Environmental 16s rDNA sequence.

Keywords


Lactic acid bacteria; 16s rRNA gene fragment; PCR; gram staining; sequencing

References


Arihati, D. B., Nugraheny, D. C., Kusuma, A. P., Vioreza, N., R, N. K. (2019). Pemanfaatan limbah sayuran sebagai bahan baku pembuatan pupuk cair dan pupuk kompos. Penamas Adi Budaya, 2(2).

Bartholomew, J. W., Finkelstein, H. (2014). Relationship of cell wall staining to gram differentiation. Journal of Bacteriology, 75(1): 77-84. doi: 10.1128/jb.75.1.77-84.1958

Bibiana, L. W., Bibiana. W. L. (1994). Analisis Mikroba di Laboratorium. Jakarta: PT Raja Grafindo Persada.

Bungol, I., Momuat, L. I., Kumaunang, M. (2014). Barcode DNA tumbuhan pangi (Pangium edule) berdasarkan gen matK. Jurnal MIPA UNSRAT, 2(3): 113-119.

Daniel, I. R., Azhar, M. (2019). Identifikasi fragmen gen 16s rRNA bakteri asam laktat UBC-DA-08 dari dadih. Chemistry Journal of State University of Padang, 8(1).

Delvia, F., Aditya, F., Ibrahim, A. (2015). Isolasi dan identifikasi bakteri asam laktat (BAL) dari buah mangga (Mangifera indica L). Jurnal Ilmiah Manuntung, 159-163.

Faatih, M. (2009). Isolasi dan digesti DNA kromosom. Jurnal Penelitian Sains dan Teknologi, 10(1): 61-67.

Fajriani, B., Budiharjo, A., Pujianto, S. (2018). Isolasi dan identifikasi molekuler bakteri antagonis terhadap vibro parahaemolyticus patogen pada udang litopenaeus vannamei dari produk probiotik dan sedimen mangrove di Rembang. Jurnal Biologi, 7(1): 52-63.

Francois, G. J. (2010). Agarose gel electrophoresis–aplications in clinical chemistry. Journal of Medical Biochemistry, 1: 29.

Pardosi, A. H., Irianto, Mukhsin. (2015). Respon tanaman sawi terhadap pupuk organik cair limbah sayuran pada lahan kering ultisol. Jurnal Prosiding Seminar Nasional Lahan Suboptima, 9-12.

Happy, N., Awaludin, A. P. (2018). Identifikasi molekular bakteri endofit mangrove Rhizhopora mucronata penghasil gelatinase (MMP2). JPHPI, 21(1): 143-147.

Handoyo, A., Rudiretna, A. (2000). Prinsip umum dan pelaksanaan polymerase chain reaction (PCR). Jurnal Unesa, 9(1): 17-29.

Ningsih, N. P., Sari, R., Apridamayanti, P. (2018). Optimasi aktivitas bakteriosin yang dihasilkan oleh Lactobacillus brevis dari es pisang ijo. Jurnal Pendidikan Informatika dan Sains, 7(2): 233-242.

Napitupulu, H. G., Rumengan, I. F. M., Wullur, S., Ginting, E. L., Rimper, J. R. T. S. L., Toloh, B. H. (2019). Bacillus sp. sebagai agensia pengurai dalam pemeliharaan brachionus rotundiformis yang menggunakan ikan mentah sebagai sumber nutrisi. Jurnal Ilmiah Platax, 7(1): 159-169.

Promega. (2012). Wizard Genomic DNA Purification Kit, USA: Promega Corp.

Rahmiati, Mumpuni, M. (2017). Eksplorasi bakteri asam laktat kandidat probiotik dan potensinya dalam menghambat bakteri patogen. Journal of Islamic Science and Technologi, 3(2): 141-149.

Sasmita, Halim, A., Sapriati, A. N., Kursia, S. (2018). Isolasi dan karakterisasi bakteri asam laktat dari liur basa (limbah sayur bayam dan sawi). Journal Farmasi, 10(2: 141-151.

Suryani, Y., Hernaman, I., Ningsih. (2017). Pengaruh penambahan urea dan sulfur pada limbah padat bioetanol yang difermentasi Em-4 terhadap kandungan protein dan serat kasar. Jurnal Ilmiah Peternakan Terpadu, 5(1): 13-17.

Thoyibatun, N., Surya, P. R. (2012). Identifikasi spesies isolat bakteri S1 dengan metode analisa sekuen fragmen gen 16s rDNA. Jurnal Teknik POMITS, 1(1): 1-6.

Widyastuti, D. A. (2017). Isolasi DNA Kromosom Salmonella sp dan Visualisasinya pada Elektroforesis Gel Agarosa. 311-317.


Full Text: PDF

DOI: 10.15408/jkv.v9i1.29466

Refbacks

  • There are currently no refbacks.


Copyright (c) 2023 Assyifa Junitasari

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.