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Optimization of Ultrasonic Assisted Extraction Process on Antioxidant Activity of Honje Fruit Extract (Etlingera elatior) Using Surface Response Method

Artta Gracia Malau, Asri Widyasanti, Selly Harnesa Putri

Abstract


Honje fruit (Etlingera elatior) contains bioactive compounds as antioxidants. An antioxidant can be obtained by extraction. This research aimed to determine the best combination of solvent amount and extraction time to produce the optimal yield and antioxidant activity. The extraction method was UAE (Ultrasound Assisted Extraction) using ethanol 96% solvent amount 125 mL to 250 mL and time level of 30 to 60 minutes. The RSM (Response Surface Methodology) in the Design Expert 11 application was used to arrange the extraction combination treatment, which resulted in 13 runs. Parameters analyzed were total yield, antioxidant activity, pH, specific gravity, and color. The results showed that total yield was revealed quadratic, Y1 = 19.05 – 1.76A + 0.32B – 0.002AB + 0.023A2 – 0.0005B2 and the antioxidant activity was revealed linear, Y2 = 408.147 – 6.424A + 0.326B. The optimum treatment was achieved in the amount of solvent 174.815 ml and extraction time of 60 minutes resulted in a total yield of 17.125% and antioxidant activity of 77.55 ppm that could be classified as strong.

Keywords


Antioxidant; honje fruit extract; response surface methodology; ultrasound assisted extraction

References


Bermudez-Aguirre, Daniela & Barbosa-Cánovas, G. (2011). Recent Advances in Emerging Nonthermal Technologies. 285-323. https://doi.org/10.1007/978-1-4419-7475-4_13.

Chemat. F.. Zill-E-Huma. Khan. M. K. (2011). Applications of ultrasound in food technology: Processing. preservation and extraction. Ultrasonics Sonochemistry. 18(4). 813–835. https://doi.org/10.1016/j.ultsonch.2010.11.023

Guenther. E. 1987. Minyak Atsiri Jilid 1 (Penerjemah S. Ketaren). Jakarta: Penerbit Universitas Indonesia.

Jabbar. A.. Wahyuni. W.. Malaka. M. H.. & Apriliani. A. (2019). Aktivitas Antioksidan Ekstrak Etanol Buah. Daun. Batang Dan Rimpang Pada Tanaman Wualae (Etlingera Elatior (Jack) R.M Smith). Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal). 5(2). 189–197. https://doi.org/10.22487/j24428744.2019.v5.i2.13671

Ksouri. R.. Megdiche. W.. Boulaaba. M.. Smaoui. A.. & Abdelly. C. (2008). Influence of biological. environmental and technical factors on phenolic content and antioxidant activities of Tunisian halophytes. Comptes Rendus Biologies. 331(11). 865–873. https://doi.org/https://doi.org/10.1016/j.crvi.2008.07.024

Lapornik B.. Prošek M.. Golc Wondra. (2005). Comparison of extracts prepared from plant by-products using different solvents and extraction time. Journal of Food Engineering. 71(2). 214–222. https://doi.org/https://doi.org/10.1016/j.jfoodeng.2004.10.036

Mariana.W. Simon. B. W. Endrika. W. (2017). Optimasi Formulasi dan Karakterisasi Fisikokimia dalam Pembuatan Daging Restrukturisasi Menggunakan Response Surface Methodology (Konsentrasi Jamur Tiram serta Gel Porang dan Karagenan). Jurnal Pangan dan Agroindustri Vol.5 No.4:83-91.Molyneux. 2004. The use of the stable free radical diphenylpicryl-hydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin Journal of Science. Technology.

Moharram, H. A., & Youssef, M. M. (2014). Methods for Determining the Antioxidant Activity : A Review. Alexandria Journal of Food Science and Technology, 11(1), 31–41. https://doi.org/10.12816/0025348

Molyneux. (2004). The use of the stable free radical diphenylpicryl-hydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin Journal of Science. Technology.

Montgomery, DC. (1991). Design and Analysis of Experiments 5th edition. New York: John Wiley & Sons, Inc.

Naufalin, R., Jenie, B. S. L., Kusnandar, F., Sudarwamto, M., & Rukmini, H. (2010). Antibacterial Activity of Kecombrang Flower Extract Toward Pathogenic and Food Spoilage Bacteria. Jurnal Teknologi Dan Industri Pangan, 16(2), 119–119. https://doi.org/10.6066/481

Oramahi. H.A. (2008). Teori dan Aplikasi Penggunaan RSM (Response Surface Methodology). Yogyakarta: Penerbit Ardana Media.

Radi, M. & Ramli, Youssef & Karbane, M. & Elalami, Aziz & Karrouchi, Khalid & Bekkali, A. & Benaji, Brahim & Issmaili, S. & Bakhous, K. (2014). Optimization and validation of a method for determination of ibuprofen by HPLC in different pharmaceutical forms: Tablet, Syrup, Gel and suppository. Journal of Chemical and Pharmaceutical Research, 6, 301–304. https://doi.org/10.1016/0891-5849(94)00240-K

SNI 06-2385-2006 Minyak Nilam. (2006). Jakarta. Retrieved from www.bsn.go.id

Tristantini. D.. Ismawati. A.. Pradana. B. T.. & Gabriel. J. (2016). Pengujian Aktivitas Antioksidan Menggunakan Metode DPPH pada Daun Tanjung (Mimusops elengi L). Universitas Indonesia. 2.

Wardiyati. S. (2004). Pemanfaatan Ultrasonik Dalam Bidang Kimia. Tangerang: Puslitbang IPTEK Bahan BATAN.

Wingate. D. (1989). Martindale: The extra pharmacopeia (29th ed). Gut. 30(12). 1804–1804.

Živković. L.. Bajić. V.. Dekanski. D.. Čabarkapa-Pirković. A.. Giampieri. F.. Gasparrini. M.. Mazzoni. L.. Potparević. B. S. (2018). Manuka honey attenuates oxidative damage induced by H2O2 in human whole blood in vitro. Food and Chemical Toxicology : An International Journal Published for the British Industrial Biological Research Association. 119. 61–65.https://doi.org/10.1016/j.fct.2018.05.034.


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DOI: 10.15408/jkv.v7i2.21396

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