Ethyl Acetate Fraction of Moringa oleifera Leaves Induces Cell Cycle Arrest on T47D Breast Cancer Cell via G0/G1 through Cyclin D1 Expression

Riza Apriani, Tati Herlina, Shabarni Gaffar


Cancer is a disease that is the leading cause of death worlwide. Exploration of a natural product containing anticancer agent provides a promising line for research on cancer. One of plant that used in traditional medicine for the cancer treatment is Moringa oleifera. The previous study has reported that the n-hexane fraction of M. oleifera leaves induce apoptosis and cell cycle arrest in T47D cells. Based on the preliminary result, ethyl acetate fraction of M. oleifera (EMO) leaves showed medium cytotoxic activity on T47D cells with IC50 values of 243.58 μg/mL and induced apoptosis cell death. This study was carried out to investigate the anticancer activity mechanism on the cellular and molecular basis of EMO against T47D breast cancer cell line by observing cell cycle progression and Cyclin D1 expression level. Analysis of the cell cycle was performed using flow cytometry and cyclin D1 expression was analyzed using the immunocytochemistry method. The result shows that EMO induced cell cycle arrest in G0/G1 phases. Immunocytochemistry assay showed that the EMO decreased expression of  cyclin D1. This result indicates that EMO has a potential compound to be explored as chemo preventive agent for breast cancer.


Breast cancer, cell cycle, cyclin D1, Moringa oleifera, T47D cell


Alao, J.P. (2007). The regulation of cyclin D1 degradation: roles in cancer development and the potential for therapeutic invention. Molecular Cancer, 6(24), 1-16.

Al-Asmari, A. K., Albalawi, S. M., Athar, M. T., Khan, A. Q., Al-Shahrani, H. & Islam, M. (2015). Moringa oleifera as an anti-cancer agent against breast and colorectal cancer cell lines. Plos ONE, 10 (8), 1-14.

Anwar, F., Latif, S., Ashraf, M. & Gilani, A.H. (2007). Moringa oleifera: a food plant with multiple medicinal uses. Phytother. Res., 21, 17–25.

Apriani, R., Gaffar, S. & Herlina, T. (2019). Cytotoxic Activity of Ethyl Acetate Fraction Moringa oleifera Leaves and Its Effect on Apoptosis Induction Against T47D Breast Cancer Cell Line. Jurnal Farmakobahari, 10(1), 9 – 16.

Cheenpracha, S., Park, E.J., Yoshida, W.Y., Barit, C., Wall, M., Pezzuto, J.M. & Chang, L.C. (2010). Potential anti-inflammatory phenolic glycosides from the medicinal plant Moringa oleifera fruits. Bioorg Med Chem, 18, 6598–6602

Coles, C., Condie, A., Chetty, U., Steel, M., Evans, H.J. & Prosser, J. (1992). p53 Mutation in Breast Cancer, Cancer Research. 52.

Cooper, G.M. & Hausman, R.E. (2007). The Cell A Molecular Approach (4th ed). Washington, D.C., USA.

Fahey, J. (2005). Moringa oleifera: A review of the medical evidencefor its nutritional, therapeutic, and prophylactic properties. part 1, Trees for Life Journal, 1(5).

Gaffar, S., Apriani, R. & Herlina, T. (2019). n-Hexane fraction of Moringa oleifera Lam. leaves induces apoptosis and cell cycle arrest on T47D breast cancer cell line. Journal of Pharmacy and Pharmacognosy Research, 7(3), 173-183.

Ganatra, T.H., Joshi, U.H., Bhalodia, P.N., Desai, T.R. & Trigar, P.R. (2012). A panoramic view on pharmacognostic, pharmacological, nutitional, therapeutic and prophylactic values of Moringa oleifera Lam. IRJP, 3(6).

Goyal, B.R., Agrawal, B.B., Goyal, R.K. & Mehta, A.A. (2007). Phyto-pharmacology of Moringa oleifera Lam. An overview. Nat. Prod. Rad., 6, 347–353.

Guevara, A.P., Vargas, C., Sakurai, H., Fujiwara, Y., Hashimoto, K., Maoka, T., Kozuka, M., Ito, Y., Tokuda, H. & Nishino, H. (1999). An antitumor promoter from Moringa oleifera Lam. Mutat Res, 440, 181–188

Guevara, A.P., Vargas, C., Sakurai, H., Fujiwara, Y., Hashimoto, K., Maoka, T., Kozuka, M., Ito, Y., Tokuda, H. & Nishino, H., (1999). An antitumor promoter from Moringa oleifera Lam., Mutat Res., 440, 181–188.

Gupta, A., Gautam, M.K., Singh, R.K., Kumar, M.V., Rao, Ch.V., & Goel, R.K. (2010). Immunomodulatory effect of Moringa oleifera Lam. extract on cyclophosphamide induced toxicity in mice. Indian J Exp Biol, 48, 1157–1160.

Inbathamizh, L. & Padmini, E. (2011). Insilico studies on the enhancing effect of anticancer phytochemicals of Moringa oleifera on cellular prostatic acid phosphatase activity. Drug Invent Today, 3, 186–192.

J. Poorolajal, N. Nafissi, M. E. Akbari, H. Mahjub, N. Esmailnasab, E. Babaee. (2016). Arch Iran Med. 19

Jung I.L., Lee, J.H. & Kang, S.C. (2015). A potential oral anticancer drug candidate, Moringa oleifera leaf extract, induces the apoptosis of human hepatocellular carcinoma cells. Oncology Letters, 10(3), 1597-1604.

Jung, I. L. (2014). Soluble extract from Moringa oleifera leaves with a new anticancer activity. Plos One, 9(4).

Kamuhabwa A., Nshimo C. & Witte P. (2000). Cytotoxicity of Some Medicinal Plant Extracts Used in Tanzanian Traditional Medicine. J. Ethnopharmacol.

Leone, A., Spada, A., Battezzati, A., Schiraldi, A., Aristil, J. & Bertoli, S. (2015). Cultivation, genetic, ethnopharmacology, phytochemistry and pharmacology of Moringa oleifera leaves: an overview. International Journal Of Molecular Sciences, 16(6), 12791-12835.

Machana, S., Weerapreeyakul, N., Barusrux, S., Nonpunya, A., Sripanidkulchai, B. & Thitimetharoch, T. (2011). Cytotoxic and Apoptotic Effects of Six Herbal Plants Against The Human Hepatocarcinoma (HepG2) Cell Line. Chinese Medicine. 6(-), 39.

Madi, N., Dani, M., Abdoun, S. & Usta, J. (2016). Moringa oleifera's Nutritious Aqueous Leaf Extract Has Anticancerous Effects by Compromising Mitochondrial Viability in an ROS-Dependent Manner. Journal of the American College of Nutrition, 0(0).

Mahajan, S.G. & Mehta, A.A. (2007). Inhibitory action of ethanolic extract of seeds of Moringa oleifera Lam. on systemic and local anaphylaxis. J Immunotoxicol, 4, 287–294.

Malki, A., Elbaya, R.Y., Ali, O., Sultan, A., & Youssef, A.M. (2017). Novel Quinuclidinone Derivatives Induced Apoptosis in Human Breast Cancer via Targeting P53. Bio-organic Chemistry.

Mbikay, M. (2012). Therapeutic potential of Moringa oleifera Leaves in chronic hyperglycemia and dyslipidemia: A review, Front Pharmacol, 3.

Mehta, K., Balaraman, R., Amin, A.H., Bafna, P.A., & Gulati, O.D. (2003). Effect of fruits of Moringa oleifera on the lipid profile of normal and hypercholesterolaemic rabbits. J Ethnopharmacol, 86, 191–195.

Mishra, G. (2011). Traditional uses, phytochemistry and pharmacological properties of Moringa oleifera plant: an overview, Scholars Research Library. Der Pharmacia Lettre, 3(2), 141-164.

Panda, S., Kar, A., Sharma, P., & Sharma, A. (2013). Cardioprotective potential of N,alpha-l-rhamnopyranosyl vincosamide, an indole alkaloid, isolated from the leaves of Moringa oleifera in isoproterenol induced cardiotoxic rats: in vivo and in vitro studies. Bioorg Med Chem Lett, 23, 959–962.

Purwal,. L, Pathak, A.K. & Jain, U.K. (2010). In vivo anticancer activity of the leaves and fruits of Moringa oleifera on mouse melanoma. Pharmacologyonline, 1, 655–665.

Shunmuganm, L. (2016). Moringa oleifera crude aqueous leaf extract induces apoptosis in humas hepatocellular carcinoma cells via the upregulation of NF-kB and IL-6/STAT3 pathway, University of Kwazulu-Natal, Durban.

Sreelatha, S., Jeyachitra, A. & Padma, P.R. (2011). Antiproliferation and induction of apoptosis by Moringa oleifera leaf extract on human cancer cells. Food and Chemical Toxicology, 49, 1270-1275.

Sun, Y., Zhao, Z., Yang, Z., Xu, F., Lu, H., Zhu, Z., Shi, W., Jiang, J., Yao, P., & Zhu, H. (2017). Risk factors and preventions of breast cancer. Int J Biol Sci, 13(11), 1387-1397.

Tiloke, C., Phulukdaree, A. & Chuturgoon, A.A. (2013). The antiproliferative effect of Moringa oleifera crude aqueous leaf extract on cancerous human alveolar ephitelial cells., BMC. Comp & Altern. Med., 13, 226.

Verma, A.R., Vijayakumar, M., Mathela, C.S., & Rao, C.V. (2009). In vitro and in vivo antioxidant properties of different fractions of Moringa oleifera leaves. Food Chem. Toxicol, 47, 2196–2201.

Welch, R.H. & Tietje, A.H. (2017). Inverstigation of Moringa oleifera leaf extract and its cancer-selective antiproliferative properties, Journal of the South Carolina Academy of Science, 15(2).

WHO, Geneva, Switzerland Breast Cancer. (2018).

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DOI: 10.15408/jkv.v6i1.15431


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