Microencapsulation of Lime Peel Essential Oils (Citrus aurantifolia) with Complex Coacervation Methods using Gelatin/Sodium Alginate Coating
Abstract
Lime (Citrus aurantifolia) peel essential oils are complex metabolites mainly used as natural preservatives. The principal constituents include d-limonene, β-pinene, and terpineol. Moreover, essential oils (EOs) are potentially known volatile and unstable compounds, hence, they require proper packaging techniques, e.g., microencapsulation. The purpose of this study is to evaluate the binary methods of lime peel essential oils microencapsulation using complex coacervation. This involves the application of gelatin and sodium alginate coatings under two specific conditions, termed core/coating ratio 1:1.2, with modified stirring and core/coating ratio 1:2.0, with stirring speed of 600 rpm, respectively. The results showed the EOs preparation generated 0.70% yield characterized by good quality, fresh aroma, and pale-yellow coloration. Also, microcapsules delivered 37.06% yield, 39.18% oil content, 31.53% encapsulation efficiency (EE), and less smooth-edged SEM surface under condition I. Meanwhile, condition II demonstrated 51.46% yield, 58.66% oil content, 92.04% encapsulation efficiency (EE), and flat-curved, SEM surface. Furthermore, it was carefully observed condition II produced more superior microcapsules
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References
Al-Aamri M.S., Al-Abousi, N.M., Al-Jabri, S.S., Alam, T., dan Khan, S.A. 2018. Chemical composition and in-vitro antioxidant and antimicrobial activity of the essential oil of Citrus aurantifolia L. leaves grown in eastern Oman. Journal of Taibah University Medical Sciences.13(2), 108-112.
Bastos LPH, Vicente J, Correa dos Santos CH, Geraldo de Carvalho M, Garcia-Rojas EE. 2020. Encapsulation of black pepper (Piper nigrum L.) essential oil with gelatin and sodium alginate by complex coacervation. Food Hydrocolloids. 102: 1-8.
Costa R, Bisignanoa C, Filocamoa A, Grassoa E, Occhiutoa F, & Spadaro F. 2014. Antimicrobial activity and chemical composition of Citrus aurantifolia (Christm.) Swingle essential oil from Italian organic crops. Journal of Essential Oil Research. 26 (6): 400-408.
Darjazi BB. 2014. The Effect of Geographical Location on Mexican Lime (Citrus aurantifolia) Peel Components. International Journal of Agriculture Innovations and Research. 3 (1): 2319-1473.
Devi N, Hazarika D, Deka C, & Kakati DK. 2012. Study of Complex Coacervation of Gelatin A and Sodium Alginate for Microencapsulation of Olive Oil. Journal of Macromolecular Science, Part A: Pure and Applied Chemistry. 49: 936–945.
Devi N, & Kakati DK. 2013. Smart porous microparticles based on gelatin/sodium alginate polyelectrolyte complex. Journal of Food Engineering. 117: 193–204.
Enejoh OS, Ogunyemi IO, Bala MS, Oruene IS, Suleiman MM, & Ambali SF. 2015. Ethnomedical Importance of Citrus aurantifolia (Christm) Swingle. The Pharma Innovation Journal. 4 (9): 01-06.
Gamarra F, Sakanaka L, Tambourgi E, & Cabral F. 2006. Influence on the quality of essential lemon (Citrus aurantifolia) oil by distillation process. Brazilian Journal of Chemical Engineering. 23 (01): 147–151.
Jafari S, Esfahani S, Fazeli MS, Jamalifar H, Samadi M, Samadi N, Najarian Toosi A, Shams Ardekani MR, & Khanavi M. 2011. Antimicrobial Activity of Lime Essential Oil Against Food-borne Pathogens Isolated from Cream-filled Cakes and Pastries. International Journal of Biological Chemistry. 5 (4): 1-8.
Julaeha E, Nugeraha R, Nurzaman M, Kurnia D, Wahyudi T, & Rosandi Y. 2018. Characterization of Ethyl Cellulose (EC) Microcapsules for Lime Oil Encapsulation. Journal of Physics: Conference Series. 1080: 1-5.
Lemos YP, Marfil PHM, & Nicoletti VR. 2017. Particle size characteristics of buriti oil microcapsules produced by gelatin-sodium alginate complex coacervation: Effect of stirring speed. International Journal of Food Properties. 20 (S2) S1438–S1447
Li Y, Jia H, Cheng Q, Pan F, & Jiang Z. 2011. Sodium Alginate–Gelatin Polyelectrolyte Complex Membranes with Both High Water Vapor Permeance and High Permselectivity. Journal of Membrane Science. 375(1-2): 304-312.
Lin Y, Chuang C, Chen H, & Yang K. 2019. Lime (Citrus aurantifolia (Christm.) Swingle) Essential Oils: Volatile Compounds, Antioxidant Capacity, and Hypolipidemic Effect. Foods. 8 (398): 1-11.
Mahato N, Sharma K, Koteswararao R, Sinha M, EkRaj Baral E, & Cho MH. 2017. Citrus Essential Oils: Extraction, Authentication and Application in Food Preservation. Critical Reviews in Food Science and Nutrition. Taylor & Francis. 59 (4): 611-625.
Preedy VR. 2016. Essential Oils in Food Preservation, Flavor and Safety. Elsevier. London.
Razak A, Djamal A, & Revilla G. 2013. Uji Daya Hambat Air Perasan Buah Jeruk Nipis (Citrus aurantifolia S.) terhadap Pertumbuhan Bakteri Staphylococcus aureus Secara In Vitro. Jurnal Kesehatan Andalas. 2 (1): 5-8.
Rojas, J. 2015. Improved Polymer Functionality by Cross-linking with Glutaraldehyde to Achieve Controlled Drug Release. In Excipient Application in Formulation Design and Drug Delivery. Springerlink. New York.
Rozenblat J, Magdassi S, & Garti N. 1989. Effect of Electrolytes, Stirring and Surfactants in The Coacervation and Microencapsulation Processes in Presence of Gelatin. Journal Microencapsulation. 6 (4): 515-526.
Shaddel R, Hesari J, Azadmard-Damirchi S, Hamishehkar H, Fathi-Achachlouei B, & Huang Q. 2017. Double Emulsion Followed by Complex Coacervation as A Promising Method for Protection of Black Raspberry Anthocyanins. Food Hydrocolloids. 77: 803-816.
Silva PT, Fries LLM, Menezes CR, Holkem AT, Schwan CL, Wigmann ÉF, Bastos JO, Silva CB. 2014. Microencapsulation: concepts, mechanisms, methods and some applications in food technology. Ciência Rural. 44 (7): 1304-1311.
Suryawanshi JAS. 2011. An overview of Citrus aurantium used in treatment of various diseases. J. Plant Sci. 5: 390–395.
Tang Y, Scher HB, & Jeoh T. 2020. Industrially Scalable Complex Coacervation Process to Microencapsulate Food Ingredients. Innovative Food Science and Emerging Technologies. 59: 1-8. Al-Aamri
Timilsena YP, Akanbi TO, Khalid N, Adhikari B, & Barrow CJ. 2018. Complex Coacervation: Principles, Mechanisms and Applications in Microencapsulation. International journal of biological macromolecules. 121: 1276-1286.
Wahyudi T, Mulyawan AS, Kasipah C, Prayudie U, & Julaeha E. 2017a. Pembuatan Mikrokapsul Minyak Jeruk (Citrus aurantifolia) untuk Aplikasi pada Penyempurnaan Tekstil. Arena Tekstil. 32 (1): 1-8.
Wahyudi T, Mulyawan AS, Sugiyana D, & Julaeha E. 2017b. Imobilisasi Mikrokapsul Minyak Jeruk Nipis (Citrus aurantifolia) pada Kain Kapas. Arena Tekstil. 32 (2): 103-110.
Wang L, Yang S, Cao J, Zhao S, & Wang W. 2016. Microencapsulation of Ginger Volatile Oil Based on Gelatin/Sodium Alginate Polyelectrolyte Complex. Chemical and Pharmaceutical Bulletin. 64: 21–26.
Xiao Z, Liu W, Zhu G, Zhou R, & Niu Y. 2014. A Review of The Preparation and Application of Flavour and Essential Oils Microcapsules Based on Complex Coacervation Technology. Journal of the Science of Food and Agriculture. 94: 1482–1494.
Yan C & Zhang W. 2014. Coacervation Processes. In Gaonkar A, Vasisht N, Khare A, & Sobel R. Microencapsulation in the Food Industry. 1st Edition. Academic Press. United States.
DOI: 10.15408/jkv.v6i1.14618
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