Characteristic of Allergen Protein of Nila Baby Fish (Oreochromis niloticus) and Its Processed Product
Abstract
Allergen protein is a protein that could triggers allergy reaction. This study purposes to examine the characteristic of allergen protein of unripe, boiled, and fried nila baby fish. Characteristic of allergen protein were observed by electrophoresis and immunoblotting method, while the determination of protein concentration was observed by Bradford method. Identification of the used nila baby fish was accomplished in Faculty of Fishery and Marine Science of IPB. The result of identification showed that the used nila baby fish was Oreochromis niloticus. The proximate analysis of unripe, boiled, and fried nila baby fish resulted water content ranged from 19.16%-23.68%, fat content ranged from 1.03%-20.44% and carbohydrate content ranged from 0.16%-20.27%. Protein concentration of unripe, boiled, and fried nila baby fish extract respectively were 1963.45 mg/L; 699.82 mg/L; 607.79 mg/L. The band amount of allergen protein of unripe, boiled, and fried nila baby fish which was detected by electrophoresis, respectively were 16 of protein band, 26 of protein band and 16 of protein band. The immunoblotting showed that the sum of respondent who contained specific IgE that can bind with allergen protein of boiled and fried nila baby fish were more than allergen protein of unripe nila baby fish. It indicated that the processing process by boiling and frying would increase allergenicity toward nila baby fish.
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References
AOAC. 1990. AOAC: Official Methods of Analysis (Volume 1). (K. Helrich, Ed.) (15th ed., Vol. 1). USA: Association of Official Analytical Chemists.
Asia N, Suparmi, Sumarto. 2015. Studi Penerimaan Konsumen Terhadap fried Baby Nila (Oreochromis niloticus) dengan ukuran berbeda. Universitas Riau.
Bollag D, Edelstein S. 1991. Protein Method. New York: Willey-Liss.
Boye JI, Godefroy SB. 2010. Allergen Management in the Food Industry. John Wiley & Sons, Inc., Hoboken, NJ.
Cianferoni A, Spergel JM. 2009. Food allergy: review, classification and diagnosis. Allergology International. 58(4): 457–466.
Darwisito S, Junior MZ, Sjafei DS, Manalu W, Sudrajat AO. 2008. Pemberian pakan mengandung vitamin e dan minyak ikan pada induk memperbaiki kualitas telur dan larva ikan nila. Jurnal Akuakultural Indonesia. 7(1): 1-10.
Ebo DG, Kuehn A, Bridts CH, Hilger C, Hentges F, Stevens WJ. 2010. Monosensitivity to pangasius and tilapia caused by allergens other than parvalbumin. Journal of Investigational Allergology & Clinical Immunology. 20(1): 84–88.
[EMEA] European Medicines Agency (EMEA/CHMP)BWP/304831/2007). The Guideline on Allergen Products: Production and Quality Issues.
Gupta RS, Dyer AA, Jain N, Greenhawt MJ. 2013. Childhood for allergies : current diagnosis, treatment and management strategies. Mayo Clinic Proceedings. 88(5): 512-526
Hashimoto K, Watabe S, Kono M, Shiro K. 1979. Muscle protein composition of Sardine and Mackerel. Bulletin of the Japanese Society of Scientific Fisheries. 45(11): 1435-1441.
Justi K, Hayashi C, Visentainer J, de Souza N, Matsushita M. 2003. The influence of feed supply time on the fatty acid profile of Nile tilapia (Oreochromis niloticus) fed on a diet enriched with n-3 fatty acids. Food Chemistry. 80(4): 489–493.
Laemmli SDS PAGE Fanglian He Carnegie Institution at Stanford. 1970. 1: 3-6.
Liu R, Holck AL, Yang E, Liu C, Xue W. 2013. Tropomyosin from tilapia (Oreochromis mossambicus) as an allergen. Clinical & Experimental Allergy. 43(3): 365–377.
Liu R, Yang E, Liu C, Xue W. 2011. Tilapia (Oreochromis mossambicus) allergens characterized by ELISA, SDS-PAGE, 2D gels, Western blotting and MALDI-TOF mass spectrometry. International Journal of Food Sciences and Nutrition. 63(3): 259–266.
Misnan R, Murad S, Arip M, Mohamed J. 2005. Characterization of Immunoglobulin E-Binding Proteins (IgE) of Scomberomorus commerson Lacepede. Jurnal Sains Kesihatan Malaysia. 3(2); 79-87.
Misnan R, Murad S, Jones M, Taylor G, Rahman D, Arip M, Mohamed J. 2008. Identification of the major allergens of Indian scad (Decapterus russelli). Asian Pacific Journal of Allergy and Immunology. 26(4): 191-198.
Nowak-wegrzyn A. 2003. Future approaches to food allergy. Pediatrics. 111: 1672-1680.
Nowak-Wegrzyn A, Fiocchi A. 2009. Rare, medium, or well done? The effect of heating and food matrix on food protein allergenicity. Current Opinion in Allergy and Clinical Immunology. 9(3): 234–237.
Ogunji JO, Wirth M, Rennert B. 2005. Assessing the dietary amino acid requirements of tilapia. Oreochromis niloticus fingerlings. 1988: 1–9.
Saguy IS, Pinthus EJ. 1995. Oil uptake during deep-fat fring: factors and mechanism. Food Tech. 4: 142-145.
Shreffler WG, Beyer K, Chu TT. 2004. Microarray immunoassay : association of clinical history , in vitro IgE function, and heterogenity of allergenic peanut epitopes. J Allergt Clin Immunology. 113: 776–782.
Soekendarsi E, Hasyim Z, Hasan S. 2016. Perbandingan Kandungan Gizi Ikan Nila Oreochromis niloticus asal Danau Mawang Kabupaten Gowa dan Danau Universitas Hasanuddin Kota Makassar. 1: 39–46.
Sundari D, Almasyhuri, Lamid A. 2015. Pengaruh proses pemasakan terhadap protein. Media Litbangkes. 25(4): 235–242.
Suseno R, Palupi NS, Prangdimurti E. 2016. Alergenisitas sistem glikasi isolat protein kedelai-fruktooligosakarida. Agritech. 36(4): 450–458.
Toda M, Heilmann M, Ilchmann A, Vieths S. 2014. The maillard reaction and food allergies: is there a link?. Clinical Chemistry and Laboratory Medicine. 52(1): 61-67.
Zakaria FAK, Ispurwanto, Rahman. 1998. Telaah sifat alergenisitas protein udang putih (Penaeus merguensis) untuk produksi isolat alergen. Bulletin Teknologi dan Industri Pangan. 2(IX): 54–59.
DOI: 10.15408/jkv.v6i1.12885
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