Aplikasi Natural Deep Eutectic Solvent (NADES) sebagai Green Solvent pada Ekstraksi Kafein dan Katekin dari Limbah Kulit Kopi

Fitria Puspita, Isna Nurhidayati, Bella Mellisani, Fajar Amelia Rachmawati Putri, Anton Restu Prihadi

Abstract


Various studies have been conducted to support green industry by developing green solvents to replace organic solvents that are generally toxic, volatile, and harmful to human health and the environment. Environmentally friendly solvents that have been successfully developed in the 21st century are eutectic solvents and natural deep eutectic solvents (NADES). NADES can be formed from primary metabolites such as amino acids, organic acids, sugars, or choline derivatives, and has a high ability to extract polar, non-polar compounds, and several other secondary metabolites. The amount of waste generated from the process of separating coffee from its seeds is abundant but has not been used optimally. Coffee husk waste has the potential to be used as a food product because it contains many active substances, especially phenolic compounds such as caffeine and catechins. In this study, an NADES solvent based on choline chloride-proline (1:1) was successfully synthesized and used to extract phenolic compounds from coffee husk waste in the form of caffeine and catechins. The results of the HPLC analysis showed that the levels of caffeine and catechins extracted were 14.5 ppm and 61 ppm, respectively. Based on these results, caffeine and catechins in coffee skin waste were successfully extracted using NADES based on choline chloride – proline (1:1).

 


Keywords


green solvent, kafein, katekin, NADES, HPLC

References


Abbott, A.P., Capper, G., Davies, D.L., Rasheed, R.K., Tambyrajah, (2003), Novel solvent properties of choline chloride/urea mixture, Chemical Communication, 70-71. https://doi.org/10.1039/B210714G

Alanon, M.E., Ivanovic, M, Go´mez-Caravaca, A.M., Arraez-Roman, D., Carretero AS, Choline chloride derivative-bases deep eutectic liquids as a novel green alternative solvent for extraction of phenolic compounds from olive leaf, Arabian Journal of Chemistry, 13(1), 1685-1701. https://doi.org/10.1016/j.arabjc.2018.01.003

Ali, M.C., Chen, J., Zhang, H., Li, Z., Zhao, L., Qiu, H., (2019), Effective extraction of flavonoids from Lyceum barbarum L. fruits by deep eutectic solvents based ultra sound-assisted extraction, Talanta, 203, 16-22. https://doi.org/10.1016/j.talanta.2019.05.012

Andrade, K.S., Goncalves, R.T., Maraschin, M., Ribeiro-do-Valle, R.M., Martinez, J., Ferreira, S.R.S., (2012), Supercritical fluida extraction from spent coffee ground and coffee husk: antioxidant and effect of operational variables on extract composition, Talanta, 88, 544-522. https://doi.org/10.1016/j.talanta.2011.11.031

Dai, Y., Ho Row, K., (2019), Application of natural deep eutectic solvent in the extraction of quercetin from vegetables, Molecules, 24, 1-11. https://doi.org/10.3390/molecules24122300

Duan, Li., Dou, L.L., Guo, L., Li, P., Liu, E.H., (2016), Comprehensive evaluation of deep eutectic solvents in extraction of bioactive natural products, ACS Sustainable Chemistry & Engineering, 4(4), 2405-2411. https://doi.org/10.1021/acssuschemeng.6b00091

Francisco, M., van den Bruinhorst, A., Kroon, M.C, (2012), New natural and renewable low transition temperature mixture (LLTMs) sreening as solvent for lognocelluloic bimass processing, Green Chemistry, 14, 2153-2157. https://doi.org/10.1039/C2GC35660K

Janissen, B., & Huynh, T., (2018), Chemical composition and value-adding application of coffee industry by product A review, Resources, Conservation & Recycling, 128, 110-117. https://doi.org/10.1016/j.resconrec.2017.10.001

Maimulyanti, A., Nurhidayati, I., Mellisani, B., Putri, F.A.R., Puspita, F., Prihadi, A.R., (2023), Development of natural deep eutectic solvent (NADES) based on choline chloride as a green solvent to extract phenolic compound from coffee husk waste, Arabian Journal of Chemistry, 16. https://doi.org/10.1016/j.arabjc.2023.104634

Machado, E.M.S., Rodriquez-Jasso, R.M., Teixeira, J.A., Mussatto, S.I., (2012), Growth of fungal strains on coffee industry residues with removal of pholyphenolic compounds, Biochemical Engineering Journal, 60, 87-90. https://doi.org/10.1016/j.bej.2011.10.007

Murthy, P.S., Naidu, M.M., (2012), Sustainable management of coffee industry by product and value addition A review, resources conservation and recycling, 66, 45-58. https://doi.org/10.1016/j.resconrec.2012.06.005

Narita, Y., Inouye, K., 2012, High Antioxidant Activity of coffee Silverskin Extracts Obtained by the Treatment of Coffee Silverskin Extracts Obtained by the Treatment of Coffee Silverskin with Subcritical Water, Food Chemistry, 135, 943-949.

Owczarek, K., Szczepanska, N., Plotka-Wasylka, J., Rutkowska, M., Shyshchak, O., Bratychak, M., & Namiesnik, J., (2016), Natural deep eutectic solvents in extraction process, Chemistry & Chemical Technology, 10(4), 601-606. https://doi.org/10.23939/chcht10.04si.601

Paiva, A., Craveiro, R., Aroso, I., Martins, M., Reis, R.L., & Duarte, A.R.C., (2014), Natural deep eutectic solvent-solvent for the 21 st century, Sustainable Chemistry & Engineering, 2(5), 1063-1071. https://doi.org/10.1021/sc500096j

Padmapriya, R., Tharian, J.A., Thirunalasundari, T., (2013), Coffee waste management an overview, International Journal of Current Research, 33(1), 9-16.t: https://www.researchgate.net/publication/330825000

Prihadi, A.P., Maimulyanti, A., Mellisani, B., Nurhasanah, (2020), Antioxidant fiber from coffee husk extrack and potential for nutraceutical, Rasayan Journal of Chemistry, 13(2), 955-959. http://dx.doi.org/10.31788/RJC.2020.1325613

Raudah & Ernawati, (2012) Pemanfaatan kulit kopi Arabika dari proses pulping untuk pembuatan bioethanol, Journal of Science and technology, 10 (21), 12-22. http://jurnal.pnl.ac.id/?p=916

Silva, M.O., Honfoga, J.N.B., de Medeiros, L.L., Madruga, M., Bezerra, T.K.A., (2021), Obtaining bioactive compounds from the coffee husk (Coffea arabica L) using different extraction methods, Molecules, 26(46), 1-13. https://doi.org/10.3390/molecules26010046

Wei, Z., Qi, X., Li, T., Luo, M., Wang, W., Zu, Y., Fu, Y., (2015), Application of natural deep eutectic solvent for extraction and determinantion of phenolic in Cajanus cajan leaves by ultra performance liquid chromatography, Separation and Purification Technology, 149, 237-244. https://doi.org/10.1016/j.seppur.2015.05.015

Yuniarti, E., Saputri, F.C., Mun’im, E., (2019), Application of the natural deep eutectic solvent choline chloridesorbitol to extract chlorogenic acid and caffeine from green coffee beans (Coffea canephora), Journal of Applied Pharmaceutical Science, 9, 82-90. https://dx.doi.org/10.7324/JAPS.2019.90312

Zhang, H., Thang, B., Row, K.H., (2014), Extraction of Catechin Compounds from Green Tea with a New Green Solvent, Chemical Research in Chinese Universities, 30, 37–41. http://dx.doi.org/10.1007/s40242-014-3339-0


Full Text: PDF

DOI: 10.15408/pbsj.v5i1.32256

Refbacks

  • There are currently no refbacks.