Uji Aktivitas Antibakteri Ekstrak Metanol Daun Paku Resam (Glechenia liearis Burm.) pada Tiga Bakteri Penyebab Akne Vulgaris
Abstract
Abstract: Acne vulgaris or acne is a skin disease caused by a bacterial infection. This study aimed to evaluate the antibacterial activity of methanol extract of paku resam leaves (Gleichenia linearis Burm.) on three different acne-causing bacteria Propionibacterium acnes ATCC 6919, Staphylococcus epidermidis ATCC 12228, and Staphylococcus aureus ATCC 25923. The research consisted of an antibacterial activity test using the disc diffusion method, a minimum inhibitory concentration (MIC) test, a minimum bactericidal concentration (MBC) test, and an equivalence test of antibacterial activity with clindamycin as a positive control. This study found that the methanol extract of paku resam leaves had a significant effect (p<0.05) on inhibiting the growth of the three bacteria at all concentrations tested, namely 2.5; 5; 10; 15; 20; 30%. The MIC value of the extract was lower for P. acnes ATCC 6919 and S. epidermidis ATCC 12228 by 1.63%, compared to S. aureus ATCC 25923 by 3.25%. The KBM value of methanol extract of paku resam leaves was lower for P. acnes ATCC 6919 and S. epidermidis ATCC 12228 (3.25%) compared to S. aureus ATCC 25923 (6.5%). The equivalence test showed that the antibacterial activity of the methanol extract of paku resam did not as strong as clindamycin inhibitory activity to the acne-causing bacteria. In conclusion, the methanolic extract of paku resam leaves has antibacterial activity toward three acne-causing bacteria based on the antibacterial test, MIC, and KBM tests, but its activity was still lessened than clindamycin.
Abstrak: Akne vulgaris atau jerawat merupakan salah satu penyakit kulit yang dapat disebabkan oleh infeksi bakteri. Penelitian ini bertujuan untuk mengevaluasi aktivitas antibakteri ekstrak metanol daun paku resam (Glechenia linearis Burm.) pada Propionibacterium acnes ATCC 6919, Staphylococcus epidermidis ATCC 12228 dan Staphylococcus aureus ATCC 25923 bakteri penyebab jerawat. Pengujian terdiri atas uji aktivitas antibakteri dilakukan dengan metode difusi cakram, uji konsentrasi hambat minimum (KHM), uji konsentrasi bunuh minimum (KBM) dan uji kesetaraan aktivitas antibakteri dengan antibiotik klindamisin sebagai kontrol positif. Hasil penelitian menunjukkan bahwa ekstrak metanol daun paku resam berpengaruh nyata (p<0.05) menekan pertumbuhan ketiga bakteri pada semua konsentrasi yang diujikan yaitu 2.5; 5; 10; 15; 20; 30 %. Nilai KHM ekstrak lebih rendah terhadap P. acnes ATCC 6919 dan S. epidermidis ATCC 12228 sebesar 1.63%, dibandingkan pada S. aureus ATCC 25923 sebesar 3.25%. nilai KBM ekstrak metanol daun paku resam lebih rendah terhadap P. acnes ATCC 6919 dan S. epidermidis ATCC 12228 (3.25%) dibandingkan pada S. aureus ATCC 25923 (6.5%). Hasil uji kesetaraan menunjukkan bahwa aktivitas antibakteri ekstrak metanol paku resam masih lebih rendah dibandingkan klindamisin. Sebagai kesimpulan, ekstrak metanol daun paku resam mampu menghambat pertumbuhan 3 bakteri penyebab jerawat berdasarkan uji antibakteri, KHM dan KBM, namun aktivitasnya masih lebih rendah dibandingkan klindamisin.
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References
Alvarez, L.A., de Sijpe, G.V., Desmet, S., Metsemaker, W-J., Spriet, I., Allegaert, K., Rozenski, J. (2022) ‘Ways to improve insight into clindamycin pharmacology and pharmacokinetics tailored to practice’ Antibiotics, 11, pp. 701. doi: 10.3390/antibiotics11050701.
Balouiri, M., Sadiki, M., Ibnsouda, S.K. (2016) ‘Methods for in vitro evaluating antimicrobial activity: A review’ Journal of Pharmaceutical Analysis, 6, pp. 71-79. doi: 10.1016/j.jpha.2015.11.005.
Bouarab-Chibane, L., Forquet, v., Lanteri, P., Clement, Y., Leonard-Akkari, L., Oulahal, N., Degraeve, P., Bordes, C. (2019) ‘Antibacterial properties of polyphenols: charactirization and QSAR (quantitative structure-activity relationship) models’ Front. Microbiol. 10, pp. 829. Doi: 10.3389/fmicb.2019.00829.
Breijyeh, Z., Jubeh, B., Karaman, R. (2020) ‘Resistance of Gram-negatif bacteria to current antibacterial agents and approaches to resolve it’ Molecules, 25, pp. 1340. doi: 10.3390/molecules25061340.
Claudel, J.P., Auffret, N., Leccia, M.T., Poli, F., Corvec, S., Dreno, B. (2018) ‘Staphylococcus epidermidis: A potential new player in the physiopahology of acne?’ Dermatology, 235, pp. 287-294. doi: 10.1159/000499858.
Das, K., Tiwari, R.K.S., Shrivastava, D.K. (2010) ‘Techniques for evaluation of medicinal plant products as antimicrobial agent: Current methods and future trends’ Journal of Medicinal Plants Research, 4(2), pp. 104-111. doi: 10.5897/JMPR09.030.
Dhamayanti, M., (2009) ‘Overview adolescent health problems and services, Adolescent Health National Symposia: Current Challenges in Management, 2. 2009
Heng, A.H.S., & Chew, F.T. (2020) ‘Systematic review of the epidemiology of acne vulgaris’ Scientific Report, 10, pp. 5754. doi: 10.1038/s41598-020-62715-3.
Jubeh, B., Breijyeh, Z., Karaman, R. (2020) ‘Resistance of Gram-positive bacteria to current antibacterial agents and overcoming approaches’ Molecules, 25, pp. 2888. doi: 10.3390/molecules25122888.
Krochmal, B.K., Wicher, R.D. (2021) ‘The minimum inhibitory concentration of antibiotics methods, interpretation, clinical relevance’ Pathogens, 10, pp. 165. doi: 10.3390/pathogens10020165.
Lai, H.Y., Lim, Y.Y., Tan, S.P. (2014) ‘Antioxidative, tyrosinase inhibiting and antibacteria activities of leaf extracts from medicinal ferns’ Bioscience, 73(6), pp. 1362-1366. doi: 10.1271/bbb.90018
Li, J., Xie S., Ahmed, S., Wang, F., Gu, Y., Zhang, C., Chai, X., Wu, Y., Cai, J., Cheng, G. (2017) ‘Antimicrobial activity and resistance: influencing factors’. Frontier in Pharmacology, 8, pp. 364. doi: 10.3389/fphar.2017.00364.
Liu, X.L., Xu, Y.J., Go, M.L. (2008) ‘Functionalized chalcones with basic functionalities have antibacterial activity againts drug sensitive Staphylococcus aureus’ Eur. J. Med. Chem. 43, pp. 1681-1687. doi: 10.1016/j.ejmech.2007.10.007
Majdanik, M.M., Kepa, M. Wojtyczka, R.D., Idzik, D., Wasik, T.J. (2018) ‘Phenolic compound disminish antibiotic resistence of Staphylococcus aureus clinical strains’ Int. J. Environ. Res. Public Health, 15, pp 2321. doi: 10.3390/ijerph15102321.
Morales, G., Sierra, P., Mancilla, A., Paredes, A., Loyola, L.A., Gallardo, O., Borquez, J. (2003) ’Secondary metabolites from four medicinal plants from Northen Chile: antimicrobial activity and biotoxicity against Artemia salina’ J. Clin. Chem. Soc, 48, pp. N2. doi: 10.4067/S0717-97072003000200002.
Nasution, J., Masitah, P.D., Riyanto. (2016) ‘Kajian etnobotani tumbuhan obat oleh etnis masyarakat di dusun Aras Napal Kiri dan Dusun Aras Napal Kanan Desa Bukit Mas Kecamatan Besitang Kabupaten Langkat’ Jurnal Biosains, 2(2), pp. 91-96. doi: 10.24114/jbio.v2i2.4225.
Nguyen, T.A., Eichenfield, L.F. (2015) ‘Profile of clindamicin phosphate 1.2%/benzoyl peroxide 3.75% aqueous gel for the treatment od acne vulgaris’ Clinical Cosmetic and Investigational Dermatology, 8, pp. 549-554. doi:10.2147/CCID.S79628 .
Othman, R., Ramya, R., Hassan, N.M., Kamoona, S. (2020) ‘Qualitative and quantitative phenolic compounds analysis of Dicranopteris linearis different fractional polarities leaves extract’ Journal of Pharmacy and Nutrition Sciences, 10, pp. 7-12. doi: 10.29169/1927-5951.2020.10.01.2.
Rao, K.N., Venkatachalam, S.R. (2000) ‘Inhibition of dihydrofolate reductase and cell growth activity by the phenanthroindolizine alkaloids pergularinine and tylophorinidine: the in vitro cytotoxicity of these plant alkaloids and their potential as antimicrobial and anticancer agents’ Toxicol in vitro, 14, pp. 53-59. doi: 10.1016/s0887-2333(99)00092-2
Rizal, R. (2017) ‘Uji aktivitas antioksidan daun paku resam (Gleichenia linearis (Burm. F.) S. W. Clarke) dengan metode DPPH’ Borneo Journal of Pharmascientech, 1(2), pp. 1-10.
Sanjay, R. (2011) ‘Acne Vulgaris treatment: The current scenario’ Indian J. Dermatol, 56(1), pp. 7-13. doi: 10.4103/0019-5154.77543.
Sari, L., Jusuf, N.K., Putra, I.B. (2020) ‘Bacterial identification of acne vulgaris’ Bali Med. J, 9(3), pp. 753-756. doi: 10.15562/bmj.v9i3.1737.
Skyes, J.E., Rankin, S.C. (2014) Isolation and identification of aerobic and anaerobic bacteria, in Canine and Feline Infectious Disease. California: Elsevier Inc.
Surjowardojo, P., Susilorini, T.E., Sirait, G.R.B. (2015) ‘Daya hambat dekok kulit apel Manalagi (Malus sylvestrs Mill.) terhadap pertumbuhan Staphylococcus aureus dan Pseudomonas sp. penyebab mastitis pada sapi perah’ J. Ternak Tropika, 16(2), pp. 40-48. doi: 10.21776/ub.jtapro.2015.016.02.6.
Tako, M., Kerekes, E.B., Zambrano, C., Kotogan, A., Papp, T., Krisch, J., Vagvolgyi, C. (2020) ‘Plant phenolics and phenolic-enriched extracts as antimicrobial agents against food-contaminating microorganisms’ Antioxidants, 9, pp. 165. doi: 10.3390/antiox9020165.
Thomas, T., Kurup, G.M., Sadasivan, C. (2007) ‘Antibacterial activity of Dicranopteris linearis under in vitro conditions’ STAR: Int. Journal (Sciences). 1(2), pp. 191-195.
Winda, G.W., Budhi, S., Sisilia, L. (2015) ‘Etnobotani masyaralat desa saham (Studi kasus di Desa Saham, Kecamatan Sengah Temila Kabupaten Landak, Kalimantan Barat’ J. Hutan Lestari, 4(1), pp. 1-8. doi: 10.26418/jhl.v4i1.14206.
DOI: 10.15408/pbsj.v4i2.30474
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