The Effect of Betel Leaf Extract on The Growth of Colletotrichum capsici in Red Chili
Abstract
Abstrak
Tanaman cabai merupakan jenis sayuran penting yang banyak dibutuhkan oleh masyarakat. Namun, berdasarkan data Badan Pusat Statistik menunjukkan bahwa produksi cabai mengalami penurunan yang salah satunya disebabkan oleh Colletotrichum capsici. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak daun sirih terhadap pertumbuhan C. capsici. Penelitian ini dilakukan menggunakan Rancangan Acak Lengkap (RAL) dengan 3 kali pengulangan. Proses ekstraksi daun sirih dilakukan dengan metode maserasi dengan pelarut etanol 96% dan uji antifungi dilakukan dengan metode difusi Kirby-Bauer. Konsentrasi ekstrak daun sirih yang digunakan adalah 5%, 10%, 15%, 20%, dan kontrol negatif menggunakan dimetil sulfoksida (DMSO). Data yang diperoleh dianalisis secara statistik menggunakan uji One-Way Anova dan uji lanjut Least Significant Difference (LSD). Hasil penelitian ini menunjukkan bahwa konsentrasi ekstrak daun sirih 20% menghasilkan zona hambat terbesar, yaitu 0,84 mm. Berdasarkan hasil pengujian disimpulkan bahwa ekstrak daun sirih memiliki aktivitas antifungi dalam menekan pertumbuhan C. capsici.
Abstract
Chili plants are important vegetables normally used by the community. However, based on the data of Badan Pusat Statistik, chili production is decreasing because of many factors, including Colletotrichum capsici pathogen. This study was aimed to determine the effect of betel leaf extract on the growth of C. capsici. This research was conducted using a completely randomized design with 3 replications. The extraction process was carried out by the maceration method with 96% ethanol solvent and the antifungal test was performed by the Kirby-Bauer diffusion method. The concentration of betel leaf extract used was 5%, 10%, 15%, 20%, and dimethyl sulfoxide (DMSO) was used as the negative control. The data obtained were analyzed statistically using the One-Way Anova test and the Least Significant Difference (LSD) posthoc test. The results of this study showed that the concentration of 20% betel leaf extract produced the largest inhibition zone of 0.84 mm. Based on the test results, it is concluded that betel leaf extract had antifungal activity in suppressing the growth of C. capsici.
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DOI: https://doi.org/10.15408/kauniyah.v14i2.21919 Abstract - 0 PDF - 0
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