AKTIVITAS ANTIBAKTERI ACTINOMYCETES ASAL DESA CEMPAKA KAPUAS HULU KALIMANTAN BARAT TERHADAP ENTEROPATOGENIK GASTROENTERITIS

Abdullah Abdullah, Ihsan Almuhardi, Antoni Antoni, Rahmawati Rahmawati

Abstract


Abstrak

Antibiotik yang digunakan secara tidak tepat dapat meningkatkan jumlah bakteri penyebab gastroenteritis yang resisten terhadap antibiotik. Beberapa kelompok mikroorganisme tanah diketahui memiliki potensi menghasilkan senyawa aktif untuk menghambat dan membunuh bakteri patogen, seperti Actinomycetes. Tujuan dari penelitian ini adalah untuk mengetahui potensi antibiotik Actinomycetes  terhadap bakteri penyebab gastroenteritis yang diisolasi dari sawah tandus di Desa Cempaka Baru, Kabupaten Kapuas Hulu, Kalimantan Barat. Proses penelitian dilakukan dari isolasi Actinomycetes dari sawah tandus, karakterisasi, serta pengujian kemampuan antibiotik terhadap bakteri Escherichia coli ATCC 25922, Salmonella enterica ATCC 14028 dan Staphylococcus aureus ATCC 25923 menggunakan metode sumuran pada media Mueller Hinton Agar. Hasil identifikasi berdasarkan karakteristik morfologi koloni, sel, dan biokimia diperoleh 1 isolat bakteri, yaitu genus Nocardia sp. ATS-4.1 yang mampu menghambat pertumbuhan bakteri-bakteri uji yang dibuktikan adanya rata-rata zona hambat yang terbentuk, yakni 14,51 mm; 16,16 mm; dan 11,10 mm. Hasil uji statistik Friedman diperoleh nilai Asymp. Sig 0,10>0,05, sehingga menunjukkan bahwa pemberian cairan kultur isolat Nocardia ATS-4.1 memberikan pengaruh yang tidak berbeda nyata antara hambatan terhadap bakteri E. coli, S. enterica, dan S. aureus. Hal ini dapat disimpulkan bahwa isolat Nocardia sp. ATS-4.1 berpotensi menghasilkan antibakteri yang setara terhadap bakteri E. coli, S. enterica, dan S. aureus penyebab gastroenteritis.

Abstract

Using antibiotics incorrectly increases the number of resistant bacteria to gastroenteritis. Soil microorganisms are known to have the potential to produce active compounds to inhibit and kill pathogenic bacteria,  for example, Actinomycetes. The purpose of this study was to determine the potential of antibiotics Actinomycetes isolated from rice fields in Cempaka Baru, Kapuas Hulu Regency, West Kalimantan, against gastroenteritis bacteria. The research order was carried out by isolating Actinomycetes from barren rice fields, and then characterizing and testing the ability of antibiotics against Escherichia coli ATCC 25922, Salmonella enterica ATCC 14028, and Staphylococcus aureus ATCC 25923 using the well method on Mueller Hinton Agar. The identification results based on morphological characteristics of colonies, cells and biochemistry showed that one of the bacteria was from genus Nocardia sp. ATS-4.1 which was able to inhibit the three test bacteria by the average inhibition zone 14.51 mm; 16.16 mm; and 11.10 mm, respectively. Friedman’s statistical test resulted in values asymp. sig 0.10>0.05 which showed that the isolate gave inhibition differences insignificantly among the bacteria. In conclusion, isolate Nocardia sp. ATS-4.1 able to produce equal antibacterial activity against bacteria E. coli, S. enterica and S. aureus caused gastroenteritis.


Keywords


Actinomycetes; Antibakteri; Gastroenteritis; Nocardia; Actinomycetes; Antibacterial; Gastroenteritis; Nocardia

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References


Adegboye, M., & Babalola, O. O. (2015). Evaluation of antibiotic biosynthetic potential of Actinomycetes isolates to produce antimicrobial agents. British Microbiology Research Journal, 7(5), 244-254.

Agus, S. (2008). Lahan gambut potensi untuk pertanian dan aspek lingkungan. Bogor: Balai Penelitian Tanah dan World Agroforestry Center.

Alimuddin., Widada, J., Asmara, W., & Mustofa. (2011). Antifungal production of a strain of Actinomycetes spp. Isolated from the rhizosphere of cajuput plant: Selection and detection of exhibiting activity against tested fungi. Indonesian Journal of Biotechnologi, 16(1), 1-10.

Bacon, K., Boyer, R., Denbow, C., Keefe, S., Neilson, A., & Williams, R. (2017). Antibacterial activity of jalapeño pepper (Capsicum annuum var. annuum) extract fractions against select foodborne pathogens. Food Science & Nutrition, 5(3), 730-738.

Balakrishna, G., Shanker, A. S., & Pindi, P. K. (2012). Isolation of phosphate solibulizing Actinomycetes from forest soils of Mahabubnagar District. International Organization of Scientific Research Journal of Pharmacy & Biological Sciences, 2(2), 271-275.

Berdy, J. (2005). Bioactive microbial metabolites. The Journal of Antibiotic, 58(1), 1-26.

Brooks, G. F., Carroll, K. C., Butel, J. S., Morse, S. A., & Mietzner, T. A. (2013). Medical microbiology. New York: Mc-Graw Hill Lange.

Cao, G., Xu, Z., Wu, X., Li, Q., & Chen, X. (2014). Capture and identification of the volatile components in crude and processed herbal medicines through on-line purge and trap technique coupled with GC × GC-TOF MS. Natural Product Research, 28(19), 1607-1612.

Cappucino, J. G., & Sherman, N. (2014). Microbiology: A laboratory manual. United States of America: Pearson.

Dalisay, D. S., Williams, D. E., Wang, X. L., Centko, R., Chen, J., & Raymond, J. (2013). Marine sediment-derived streptomyces bacteria from British, Columbia, Canada are a promising microbiota resource for the discovery of antimicrobial natural products, Plos One, 8(10), 1-14.

Dologhazi, J. R., & Metcalf, W. W. (2013). Comparative genomics of actinomycetes with a focus on natural product biosynthetic genes. Biomed Central Genomics, 14(611), 1-13.

Ganesan, P., Reegan, A. D., David, R. H. A., Gandhi, M. R., Paulraj, M. G., Al-dhabi, N. A., & Ignacimuthu, S. (2017). Antimicrobial activity of some Actinomycetes from Western Ghats of Tamil Nadu, India. Alexandria Journal of Medicine, 53(2), 101-110.

Harley, J. P., & Prescott, L. M. (2002). Laboratory exercises in microbiology. New York: Mc-Graw Hill Lange.

Haryani, A., Grandiosa, R., Buwono, I. D., & Santika, A. (2012). Uji efektivitas daun pepaya (Carica papaya) untuk pengobatan infeksi bakteri Aeromonas hydrophila pada ikan mas koki (Carassius auratus). Jurnal Perikanan & Kelautan, 3(3), 213-220.

Hikmatullah., Suharta, N., & Hidayat, A. (2008). Potensi sumberdaya lahan untuk pengembangan komoditas pertanian di provinsi Kalimantan Barat. Jurnal Sumberdaya Lahan, 2(1), 45-58.

Holt, J., Krig, P., Sneath, J., & Staley, S. (1994). Bergey’s manual of determinative bacteriology. United States of America: Springer.

Hoshini, Y., Mukai, A., Yazawa, K., Uno, J., Ishikawa, J., Ando, A., & Mikami, Y. (2004). A new thiazolidine-type antibiotic with zinc in its structure, designated transvalencin A, was isolated from Nocardia sp. IFM 1006 a clinical isolate from a patient with actinomycotic Nocardia transvalensis. The Journal of Antibiotics, 57(12), 797-802.

Kavitha, A., Prabhakar, P., Vijayalakshmi, M., & Venkateswarlu, Y. (2009). Production of bioactive metabolites by Nocardia levis. Letter in Applied Microbiology, 49(4), 484-490.

Kementerian Kesehatan Republik Indonesia. (2014). Profil kesehatan indonesia. Jakarta: Kementerian Kesehatan Republik Indonesia.

Kementerian Kesehatan Republik Indonesia. (2018). Profil kesehatan indonesia. Jakarta: Kementerian Kesehatan Republik Indonesia.

Kumala, T., Jayuska, A., & Ardiningsih, P. (2015). Uji awal aktivitas antimikroorganisme dari Actinomycetes 9ISP1 berasosiasi spons. Jurnal Kimia Khatulistiwa, 4(2), 30-36.

Mulyadi., & Sulistyani, N. (2013). Aktivitas cairan kultur 12 isolat Actinomycetes terhadap bakteri resisten. Kesehatan Masyarakat, 7(2), 89-96.

Murray, R., Bender, D., Botham, K., Kennelly, P., Rodwell, V., & Weil, P. (2009). Harper’s illustrated biochemistry. New York: Mc-Graw Hill Lange.

National Center for Biotechnology Information. (2019). PubChem database of chloramphenicol. (2020, January 19). Retrieved from https://pubchem.ncbi.nlm.nih.gov/compound/Chloramphenicol.

Pelczar, E. M., & Chan, E. (2005). Basic of microbiology. New York: Mc-Graw Hill Lange.

Rai, K., Khadka, S., & Shrestha, B. (2018). Actinomycetes : Isolation, characterization and screening for antimicrobial activity from different sites of Chitwan, Nepal. International Journal of Microbiology & Biotechnology, 3(1), 25-30.

Sharma, C., Agarwal, A., Mehtani, P., & Bhatnagar, P. (2014a). Characterization of Actinomycetes from soil sample of Nahargarh Hill Area, Jaipur, India for production of antimicrobial compounds and enzymes. International Journal of Advanced Biotechnology & Research, 5(3), 304-312.

Sharma, M., Dangi, P., & Choundhary, M. (2014b). Actinomycetes : Source, identification and their applications. International Journal of Current Microbiology & Applied Sciences, 3(2), 801-832.

Waluyo, L. (2008). Teknik dasar dalam mikrobiologi. Malang: Universitas Muhammadiyah Malang Press.

World Health Organization (WHO). (2014). Antimicrobial resistance: Global health report on surveillance. France: Bulletin of the World Health Organization.




DOI: https://doi.org/10.15408/kauniyah.v13i1.11731 Abstract - 0 PDF - 0

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