TLC Fingerprint Analysis and Evaluation of α-Glucosidase Inhibitory and Free Radical Scavenging Activity of Justicia gendarussa from Different Growth Locations
Abstract
Justicia gendarussa is known to have antioxidant and antidiabetic properties, which are related to the composition and concentration of its metabolites and influenced by differences in growth location. This study aims to evaluate changes in metabolite profiles and biological activities, such as the inhibition of α-glucosidase and free radical scavenging in J. gendarussa genotypes from Bogor, Cianjur, and Sukabumi. Changes in metabolite profiles were assessed using thin-layer chromatography (TLC) fingerprint analysis combined with principal component analysis (PCA). Bioautography TLC using DPPH was performed to confirm the presence of antioxidant compounds. In the TLC fingerprint analysis of J. gendarussa, we obtained 14 bands with good resolution. PCA successfully grouped J. gendarussa extracts based on growth location. The percentage of α-glucosidase inhibitory and free radical scavenging activity was significantly different, with the highest percentage of inhibition of α-glucosidase shown by the IIIS genotype (99.23%) and the highest free radical scavenging (91.35%) demonstrated by the IIB genotype. The results of TLC bioautography confirmed the presence of antioxidant compounds in J. gendarussa represented by bands with Rf 0.04, 0.32, and 0.80. This study concludes that growth location differences influence the variations in metabolite profiles, α-glucosidase inhibitory activity, and free radical scavenging activity of J. gendarussa genotypes.
Keywords
References
Fisol NHS, Cheong NDH, Mohamed E, et al. Analyses of phytochemical constituents in Justicia gendarussa extract: a mini review. Healthscope. 2022;5(2):56-63.
Vasantharaj S, Shivakumar P, Sathiyavimal S, et al. Antibacterial activity and photocatalytic dye degradation of copper oxide nanoparticles (CuONPs) using Justicia gendarussa. Applied nanoscience. 2021;4–8.doi: 10.1007/s1three204–021–01939–9.
Wahyuni DK, Huda A, Faizah S, et al. Effects of light, sucrose concentration and repetitive subculture on callus growth and medically important production in Justicia gendarussa Burm.f. Biotechnol. 2020a;27:1-9. doi:10.1016/j.btre.2020.e00473.
Ramees MK, Hamath K, Nileena F, et al. Phytochemical screening of Justicia gendarussa. IPCM. 2019;3(1): 1–6.
Mnatsakanyan MM, Queiroz EF, Marcourt L, et al. Quantitative evaluation of various preparations and extracts of the male contraceptive Justicia gendarussa and identification of a new aminobenzyl derivative. Planta Med Int Open. 2018;5(01):30–38. doi.org/10.1055/a-0584-032.
Nurcholis W, Safithri M, Marliani N, et al. Response surface modeling to optimize sonication extraction with the maceration method for the phenolic contentand antioxidant activity of Justicia gendarussa Burm f. J Appl Pharm Sci. 2023;13(10):181-187.
Zhang H, Xia Z, Xu T, et al. New compounds from the aerial parts of Justicia gendarussa Burm.f and their antioxidant and antiinflammatory activities. Nat Prod Res. 2020;35(20):3478-3486. doi: 10.1080/14786419.2019.1710708.
Pushparani V, Deepak Kumar K, Parimalam M, et al.. Screening of medicinal plant Justicia gendarussa Burm. f for its antibacterial and antioxidant activity from different localities. Int. J. Curr. Res. 2017;9(4):49063–49066.
Amalia EA , Rollando , Afthoni MH, et al. Uji aktivitas anti bakteri daun gandarusa (Justicia gendarusa burm.f) terhadap bakteri Escherichia coli dan Staphylococcus aureus. Sainsbertek Jurnal Ilmiah Sains & Teknologi. 2022;3(1):1–10.
Hikmawanti PE, Widiyanti P, Prajogo EW. In vitro–HIV activity of ethanol extract from J. gendarussa (Justicia gendarussa Burm. f) leaves. Infectious Disease Reports. 2020;12(1):51-55. doi: 10.408/idr.2020.8730.
Sinansari R, Prajogo EWB, Widiyanti P. In silico screening and biological evaluation of the compounds of Justicia gendarussa leaves extract as interferon gamma inducer: a study of anti human immunodeficiency virus (HIV) development. Afr., J. Infect. Dis. 2018;12:140-147. doi.org/10.2101/Ajid.12v1S.21.
Ummah R, Wardoyo Eko BP, Widyowati R. Mini Review: Quality Control Study of Crude Drug of Justicia gendarussa Burm. f. Leaves as Male Contraceptive. MPI. 2022;4(1):94–104
Rosa A. Simulasi docking molekuler senyawa potensial tanaman Justicia gendarussa Burm.f. sebagai antidiabetes. Bul Penelit Kesehat. 2020;48(2):117 – 122.
Suresh BN, Satish PV, Oswal RJ. Effects of Justicia gendarussa leaves in streptozotocin-induced diabetic rats. Int J Pharm Sci Res. 2020;11(7): 3300-3305. doi:10.13040/IJPSR.0975-8232.11(7).3300-05.
Kartini K, Putri RE, Budiono R. Quantification of sinensetin in Orthosiphon stamineus from various phytogeographical zones in Indonesia. J Appl Pharm. 2023;13(03):183-191.
Wahyuni WT, Sahara M, Arifa Z, et al. Thin layer chromatographic fingerprint and chemometrics analysis for identification of Phyllanthus niruri from its related species. J Indones Chem Soc. 2020b;3(1):47-52. doi:10.34311/jics.2020.03.1.47
Rafi M, Yoland SR, Septaningsih DA, et al. Identification of Sida rhombifolia from its related plants using thin-layer chromatographic analysis. Indones J Chem. 2023;23(1):21 – 32.
Rafi M, Rohaeti E, Miftahudin A, et al. Differentiation of Curcuma longa, Curcuma xanthorrhiza and Zingiber cassumunar by thin layer chromatography fingerprint analysis. Indones J Chem. 2011;11(1):71-74.
Kartini K, Dewi ER, Achmad F, et al. Thin layer chromatography fingerprinting and clustering of Orthosiphon stamineus Benth. from different origins. Pharmacogn J. 2020;12(1):168three-1691.
Syafi’i M, Rohaeti E, Wahyuni WT, et al. Analisis Sidik Jari Kromatografi Lapis Tipis Rimpang Temu Mangga (Curcuma manga). JJI. 2018;3(3):109–115.
Astuti M, Darusman LK, Rafi M. High performance thin layer chromatography fingerprint analysis of guava (Psidium guajava) leaves. J Phys Conf Ser. 2017;835:1-6. doi :10.1088/1742-6596/835/1/012018.
Shamili G, Santhi G. Identification and characterization of bioactive compounds of leaves of Justicia gendarussa Burm. F. Int J Sci Res in Biological Sciences. 2019; 6(1):145-153. doi.org/10.26438/ijsrbs/v6i1.145153.
Reich E, Schibli A. 2006. High-Performace Thin-Layer Chromatography for the Analysis of Medicinal Plants. New York (US): Thieme Medical Publishers.
Yuliana ND, Arifn AS, Rafi M. Multiple spectroscopic fngerprinting platforms for rapid characterization of α‑glucosidase inhibitors and antioxidants from some commonly consumed indonesian vegetables and spices. J Food Meas Charact. 2002;14(3). doi:10.1007/s11694-020-00418-z.
Batubara I , Trimulia R , Rohaeti E, et al. Hubungan lama distilasi, kandungan senyawa, dan bioautografi antioksidan minyak atsiri bangle (Zingiber purpureum). Indones J Essent Oil. 2018;3(1): 37-44.
Sholehah DN, Amrullah A, Badami K. Identifikasi kadar dan pengaruh sifat kimia tanah terhadap metabolit sekunder (Curcuma domestika Val.) di Bangkalan. J Ilmiah Rekayasa. 2016;9(1): 60-66.
Li Y, Konga D, Fub Y, et al. The effect of developmental and environmental factors on secondary metabolites in medicinal plants. Plant Physiology and Biochemistry. 2020;148:80–8. doi.org/10.1016/j.plaphy.2020.01.006.
Qi Zhao, Song Z, Fang X, et.al. 2016. Effect of Genotype and Environment on Salvia miltiorrhiza Roots Using LC/MS-Based Metabolomics. Molecules. 2016;21(414):1-17. doi:10.3390/molecules21040414.
Hawrył A, Hawrył M, Litwińczuk W, Bogucka-Kocka A. Thin-layer chromatographic fingerprint of selected Paulownia species with chemometrics and antioxidant activity, Journal of Liquid Chromatography & Related Technologies. 2020; 43(11-12): 367-374. doi: 10.1080/10826076.2020.1725552
Kartini K, Andriani YA, Priambodo W, et al. Validating and developing TLC-based fingerprinting for Curcuma longa L. J Pharm Pharmacogn. 2021;9(5):704-715.
Rohman A, Wijayanti T , Windarsih A, et al.. The Authentication of java turmeric (Curcuma xanthorrhiza) using thin layer chromatography and 1h-nmr based-metabolite fingerprinting coupled with multivariate analysis. Molecules. 2020;25:1-13. doi:10.3390/molecules25173928.
El Aatik A, Navarro JM, Martínez R, et.al. Estimation of global water quality in four municipal wastewater treatment plants over time based on statistical methods. Water. 2023;15(8):1-16. 1520. doi.org/10.3390/w15081520.
Lallo S, Lewerissa AC, Rafi’I A, et al. Pengaruh ketinggian tempat tumbuh terhadap aktivitas antioksidan dan sitotoksik ekstrak rimpang lengkuas (Alpinia galanga l.). MFF 2019; 23(3):118-123.
Andriansyah I, Gumilar HF, Yuliantini HF, et al. Analisis sidik jari herba pegagan (Centella asiatica (L.) Urb) di daerah Jawa Barat menggunakan metode spektrofotometri FTIR kombinasi dengan PCA. Jurnal Agrotek Ummat. 2022;9(4):287-297.
Boulila A, Sanaa A, Salem IB, et al. Antioxidant properties and phenolic variation in wide populations of Marrubium vulgare L. (Lamiaceae). Ind. Crops Prod. 2015;76:616–622.
Mahajana M, Kuirya R, Pal PK. Understanding the consequence of environmental stress for accumulation of secondary metabolites in medicinal and aromatic plants. J. Appl. Res. Med. Aromat. Plants. 2020;18:1-10. doi.org/10.1016/j.jarmap.2020.100255.
Suryani, Al Anshory AC, Marlin, et al. Variability total phenolic content and antioxidant activity of curcuma zanthorrhiza and c. aeruginosa cultivated in three different locations in west java, indonesia. Biodiversitas. 2022;23(4): 1998-2003. doi: 10.13057/biodiv/d230434.
Istiqlal MRA, Syukur M , Wahyu Y. Keragaman Genetik Karakter Kuantitatif pada Tanaman Cabai (Capsicum annuum L.) Comm. Hort. J, Februari 2019;3(1):6-12. doi.org/10.29244/chj.3.1.6-12.
Bovani RP, Liwanda N , Batubara I , et al. Phytochemical content and antioxidant capacity of ethyl acetate extracts from fifteen Orthosiphon aristatus leaves genotypes. Biodiversitas. 2024;25(2):763-769. doi.10.13057/biodiv/d250236.
Syukur M, Maharijaya A, Nurcholis, et al. Biochemical and Yield Component of Hybrid Chili (Capsicum annuum L.) Resulting from Full Diallel Crosses. Horticulturae 2023;9:620. doi.org/10.3390/horticulturae9060620.
Yu F, Wang Q, Wei S, et al.. Effect of Genotype and Environment on Five Bioactive Components of Cultivated Licorice (Glycyrrhiza uralensis) Populations in Northern China. Biol Pharm Bull. 2015;38(1):75–81.
Suryani , Al Anshory AC, Marlin , et al. Variability total phenolic content and antioxidant activity of Curcuma zanthorrhiza and C. aeruginosa cultivated in three different locations in West Java, Indonesia. Biodiversitas. 2022;23(4): 1998-2003.
Hattaya H, Hemon AF, Kisman. Pengaruh komposisi media tanam arang sekam terhadap karakter pertumbuhan dan hasil beberapa genotip tanaman okra. Crop Agro. 2022;12(2):171-180.
DOI: 10.15408/jkv.v11i1.43662
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