Detection and Identification of Pathogenic Bacteria in Monomorium sp. Ant in The Environment of Kendari City Hospital, Southeast Sulawesi

Amirullah Amirullah; Nur Arfa Yanti; Wahyuni Nurul Surachma; Suriana Suriana

doi:10.15408/kauniyah.v18i2.41032

Detection and Identification of Pathogenic Bacteria in Monomorium sp. Ant in The Environment of Kendari City Hospital, Southeast Sulawesi

Amirullah Amirullah, Nur Arfa Yanti, Wahyuni Nurul Surachma, Suriana Suriana

Abstract

Monomorium sp. ant is one of the insects that are considered pests in the hospital environment and potential to be mechanical vectors that can carry and spread pathogenic bacteria and cause nosocomial infections. This study aims to detect and identify pathogenic bacteria found in Monomorium sp. ants originating from the environment of Kendari City Hospital, Southeast Sulawesi. Sampling of Monomorium sp. was carried out by the bait/sugar trap method in 3 locations of Kendari City Hospital, namely internal inpatient rooms, nutrition installations, and laboratory installations. Detection of pathogenic bacteria was carried out by the pour plate method on selective media, namely MacConkey Agar and Mannitol Salt Agar. The identification of bacteria was carried out by numerical-phenetic analysis based on phenotypic characters using the MVSP 3.1 application. The results of the study identified 5 species of pathogenic bacteria found in Monomorium sp. ants from the Kendari City Hospital environment. Three species of bacteria were found in ants from the internal inpatient room, namely Pseudomonas aeruginosa, Stenotrophomonas maltophilia, and Brevundimonas vesicularis, and two species found in ants from nutrition installations, namely Pseudomonas luteola and Staphylococcus auricularis. No pathogenic bacteria were found in ants from the laboratory installation.

Keywords

Mechanical vector; Monomorium ant; Nosocomial infection; Pathogenic bacteria; Bakteri patogen; Infeksi nosokomial; Semut Monomorium; Vektor mekanik

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References

Al-Saffar, M. F., & Jarallah, E. M. (2019). Isolation and characterization of Pseudomonas aeruginosa from Babylon province. Biochemical & Cellular Archives, 19(1), 203-209.

Alharbi, J. S., Alawadhi, Q., & Leather, S. R. (2019). Monomorium ant is a carrier for pathogenic and potentially pathogenic bacteria. BMC Research Notes, 12, 1-5. doi: 10.1186/s13104-019-4266-4.

Ali, M. A., & Aljanaby, A. A. J. (2023). First case report of Pseudomonas luteola isolated from urinary tract infection in Babylon City, Iraq. E3S Web of Conferences, 381, 1102. doi: 10.1051/e3sconf/202338101102.

Biomerieux. (2015). Api & Id 32 identification databases booklet (Lyon (ed.)). BioMerieux SA. Retrieved from https://www.biomerieux-diagnostics.com/sites/clinic/files/9308960-002-gb-b-apiweb-booklet.pdf.

Carrasquilla, M. C., Ortiz, M. I., Amórtegui-Hernández, D., García-Restrepo, S., León, C., Méndez-Cardona, S., & González, C. (2023). Pathogens, reservoirs, and vectors involved in the transmission of vector-borne and zoonotic diseases in a Colombian region. Brazilian Journal of Microbiology, 54(2), 1145-1156. doi: 10.1007/s42770-023-00903-9.

Do Nascimento, L. E., Amaral, R. R., Ferreira, R. M. dos A., Trindade, D. V. S., Do Nascimento, R. E., Da Costa, T. S., & Souto, R. N. P. (2020). Ants (Hymenoptera: Formicidae) as potential mechanical vectors of pathogenic bacteria in a public hospital in the Eastern Amazon, Brazil. Journal of Medical Entomology, 57(5), 1619-1626.

Ha, E. T., & Heitner, J. F. (2021). Staphylococcus auricularis endocarditis: A rare cause of subacute prosthetic valve endocarditis with severe aortic stenosis. Cureus, 13(1).

Hafiz, T. A., Aldawood, E., Albloshi, A., Alghamdi, S. S., Mubaraki, M. A., Alyami, A. S., & Aldriwesh, M. G. (2022). Stenotrophomonas maltophilia epidemiology, resistance characteristics, and clinical outcomes: understanding of the recent three years’ trends. Microorganisms, 10(12), 2506.

Holt, J. G., Krieg, N. R., Sneath, P. H. A., Staley, J. T., & Williams, S. T. (2000). Bergey's manual of determinative bacteriology. Philadelphia: Lippincott Williams & Wilkins.

Hugh, R., & Leifson, E. (1963). A description of the type strain of Pseudomonas maltophilia. International Journal of Systematic and Evolutionary Microbiology, 13(3), 133-138.

Jung, B., & Hoilat, G. J. (2022). MacConkey medium. StatPearls (internet): StatPearls Publishing.

Lestari, D. N., Ginandjar, P., & Hestiningsih, R. (2019). Kontaminasi bakteri pada semut Monomorium sp. (Hymenoptera: Formicidae) yang ditemukan di ruang rawat inap kelas III rumah sakit “X” kabupaten Kendal. Jurnal Kesehatan Masyarakat, 7(1), 246-251.

Mamay, M. (2022). Penggunaan ekstrak kayu secang dan kol ungu pada media manitol salt agar untuk menumbuhkan Staphylococcus. Klinikal Sains: Jurnal Analis Kesehatan, 10(1), 62-72. doi: 10.36341/klinikal_sains.v10i1.2528.

Palleroni, N. J., & Bradbury, J. F. (1993). Stenotrophomonas, a new bacterial genus for Xanthomonas maltophilia (Hugh 1980) Swings et al. 1983. International Journal of Systematic and Evolutionary Microbiology, 43(3), 606-609.

Paramasivam, V., Paez, A., Verma, A., Landry, D., & Braden, G. L. (2022). Brevundimonas vesicularis peritonitis in a chronic peritoneal dialysis patient. Case Reports in Nephrology and Dialysis, 11(3), 314-320.

Picanco, M. C., Costa, T. L., Soares, J. R. S., de Freitas, D. R., Ramos, R. S., Júnior, P. A. S., … Rodrigues-Silva, N. (2023). Management of ant pests in urban environments. Research, Society and Development, 12(5), e23912541658-e23912541658.

Qin, S., Xiao, W., Zhou, C., Pu, Q., Deng, X., Lan, L., … Wu, M. (2022). Pseudomonas aeruginosa: Pathogenesis, virulence factors, antibiotic resistance, interaction with host, technology advances and emerging therapeutics. Signal Transduction and Targeted Therapy, 7(1), 199.

Raad, M., Haidar, M. A., Ibrahim, R., Rahal, R., Jaoude, J. A., Harmouche, C., … Riachy M. (2023). Stenotrophomonas maltophilia pneumonia in critical COVID-19 patients. Scientific Reports, 13(1), 3392.

Raoofi, S., Kan, F. P., Rafiei, S., Hosseinipalangi, Z., Mejareh, Z. N., Khani, S., … Ghashghaee A. (2023). Global prevalence of nosocomial infection: A systematic review and meta-analysis. PLoS One, 18(1), e0274248.

Ryan, M. P., & Pembroke, J. T. (2018). Brevundimonas spp: Emerging global opportunistic pathogens. Virulence, 9(1), 480–493.

Said, M. S., Tirthani, E., & Lesho, E. (2021). Stenotrophomonas maltophilia. StatPearls (Internet). Treasure Island (FL): StatPearls Publishing; 2024 Jan-. Retrieved from: https://www.ncbi.nlm.nih.gov/books/NBK572123/

Sardi, A. (2021). Infeksi nosokomial: Jenis infeksi dan patogen penyebabnya. Seminar Nasional Riset Kedokteran (SENSORIK), 2(1), 117-125.

Segers, P., Vancanneyt, M., Pot, B., Torck, U., Hoste, B., Dewettinck, D., … De Vos, P. (1994). Classification of Pseudomonas diminuta Leifson and Hugh 1954 and Pseudomonas vesicularis Büsing, Döll, and Freytag 1953 in Brevundimonas gen. nov. as Brevundimonas diminuta comb. nov. and Brevundimonas vesicularis comb, nov., Respectively. International Journal of Systematic and Evolutionary Microbiology, 44(3), 499-510.

Sekhi, R. J. (2022). Pseudomonas aeruginosa: A review article. European Scholar Journal, 3(3), 78-84.

Setianingsih, I., Ridha, M. R., Hidayat, S., & Andiarsa, D. (2017). Semut sebagai vektor mekanik bakteri di dalam Gedung Balai Litbang P2B2 Tanah Bumbu, Kalimantan Selatan: Studi pendahuluan. Journal of Health Epidemiology and Communicable Diseases, 3(2), 42-49.

Sharaf, M. R., Mohamed, A. A., Boudinot, B. E., Wetterer, J. K., Garcia, F. H., Al Dhafer, H. M., & Aldawood, A. S. (2021). Monomorium (Hymenoptera: Formicidae) of the Arabian Peninsula with a description of two new species, M. heggyi sp. n. and M. Khalidi sp. n. PeerJ, 9, e10726.

Shi, Y., Cao, Q., Sun, J., Hu, X., Su, Z., Xu, Y., … Feng, Y. (2023). The opportunistic pathogen Pseudomonas aeruginosa exploits the bacterial biotin synthesis pathway to benefit its infectivity. PLoS Pathogens, 19(1), e1011110.

Stabler, S. N., Mack, B., McCormack, G., & Cheng, M. P. (2018). Brevundimonas vesicularis causing bilateral pneumosepsis in an immunocompetent adult: A case report and literature review. The Canadian Journal of Hospital Pharmacy, 71(3), 208.

Stobnicka-Kupiec, A., Gołofit-Szymczak, M., Cyprowski, M., & Górny, R. L. (2024). Monitoring of enteropathogenic Gram-negative bacteria in wastewater treatment plants: A multimethod approach. Environmental Science and Pollution Research, 1-16.

Swings, J., De Vos, P., Van den Mooter, M., & De Ley, J. (1983). Transfer of Pseudomonas maltophilia Hugh 1981 to the genus Xanthomonas as Xanthomonas maltophilia (Hugh 1981) comb. Nov. International Journal of Systematic and Evolutionary Microbiology, 33(2), 409-413.

Tian, S., Li, K., Tang, H., Peng, Y., Xia, L., Wang, X., … Zhou, F. (2022). Clinical characteristics of Gram-negative and Gram-positive bacterial infection in acute cholangitis: A retrospective observational study. BMC Infectious Diseases, 22(1), 269. doi: 10.1186/s12879-021-06964-1.

Widianingrum, D. C., & Salasia, S. I. O. (2021). Characterization of Staphylococcus aureus isolated from subclinical mastitis of Peranakan Ettawa goat in Pekanbaru. IOP Conference Series: Earth and Environmental Science, 759(1), 12068. doi:10.1088/1755-1315/759/1/012068.

Williford, S., Heavner, M., Lambing, T., Wian, B., Ma, S., & Gonzales, J. (2018). 704: When “contaminants” become pathogens: Staphylococcus auricularis bacteremia in the critically ill. Critical Care Medicine, 46(1), 338. doi: 10.1097/01.ccm.0000528719.31637.80.

Yanti, N. A., Sembiring, L., Margino, S., Muhiddin, N. H., & Ahmad, S. W. (2019). Polyphasic identification of amylolytic bacteria producing bioplastic poly-β-hydroxybutyrate (PHB). Sains Malaysiana 48(12), 2663-2673. doi: 10.17576/Jsm-2019-4812-07.

DOI: https://doi.org/10.15408/kauniyah.v18i2.41032

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