Methane Gas Production In Buffalo Rumen Fluid Containing Citronella Residue (Cymbopogon nardus L.) by In Vitro Method

Arina Findo Sari, Wibowo Mangunwardoyo, Fery Hadi Setiawan, Irawan Sugoro

Abstract


Methane is one of the greenhouse gases produced by ruminants. One way to reduce methane is by feeding development strategies. Concentrates or forage are two possible forms of animal feed. Forage can be replaced with citronella (Cymbopogon nardus L.) residue, which has not been fully consumed as feed. The purpose of the study was to decide the production of methane gas that has residual citronella to concentrate in buffalo rumen fluid in vitro, for 48 hours. The treatments in this research were 0.4 g citronella residue (A); 0.4 g citronella residue + 0.01 g concentrate (B); 0.4 g citronella residue + 0.02 g concentrate (C); 0.01 g concentrate (D); and 0.02 g concentrate (E). For the 48-hour measurements, the highest methane gas production was treatment 98.2% (D); followed by 92.06% (E); 17.71% (C); 15.33% (A); and 13.54% (B). It can be concluded that methane gas can be reduced by residue citronella. This shows that citronella residue can lower methane gas. The study's findings are anticipated to be among the references for using agricultural product residues, particularly citronella as animal feed to reduce the methane produced by the livestock industry


Keywords


Animal feed; Citronella residue; Cymbopogon nardus L.; In vitro; Pakan ternak; Residu serai wangi

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References


Agricultural Research and Development Agency. (2014). InfoTek perkebunan: Media bahan bakar nabati dan perkebunan. Publikasi Semi Populer, 6(2), 5-8.

Aldrian, E., Puspowardoyo, S., & Haryanto, B. (2019). Emisi gas rumah kaca dari peternakan di Indonesia dengan metode tier 2 ipcc. Jakarta: Lipi Press.

Alwi, M., Suryapratama, W., & Suhartati F. M. (2013). Fermentasi ampas tebu (bagasse) menggunakan Phanerochaete chrysosporium sebagai upaya meningkatkan produk fermentasi rumen secara in vitro. Jurnal Ilmiah Peternakan, 1(2), 479-487.

Astuti, T., Sapoti, Y., Akbar, S. A., Basyirun, F., Surtina, D. (2024). The evaluation of substituting native grass with citronella waste on the digestibility of dry matter, organic matter, and crude protein in ruminants feeding. Bantara Journal of Animal Science, 6(2), 13-16.

Bayne, J. E., & Edmindson. M. A. (2021). Penyakit pada sistem pencernaan. In D. G. Pugh, A. N. Baird, M. A. Edmondson, & T. Passler (Eds.), Sheep, Goat, and Cervid Medicine, (pp. 63-96). Elsevier.

Blummel, M., Aiple, K. P., Steingass, H., & Becker, K. (1999). A note on the stoichiometrical relationship of short-chain fatty acid production and gas formation in vitro in feedstuffs widely differing quality. Journal of Animal Physiology and Animal Nutrition, 81(3), 157-167.

Castillo-Gonzălez, A. R., Burrola,-Barraza, M. E., Domininguez-Viveroz, J., & Chavez-Martinez, A. (2014). Rumen microorganisms and fermentation. Archivos de Medicina Veterinaria, 46(3), 349-361.

Olijhoek, D., & Lund, P. (2017). Methane production by ruminants. Denmark: Department of Animal Science AU-Foulum, Aarhus University.

Edi, D. N., & Haryuni, N. (2023). Estimation of greenhouse gas emission burden of livestock sector in East Java Province, Indonesia. Jurnal Teknologi Lingkungan, 24(2), 157-165.

Elihasridas, E., Zain, M., Ningrat, R. W. S., Erpomen, E., Makmur, M., Putri, E. M., & Halimatuddini, H. (2022). Evaluation of pre-ingestive citronella residue using ruminal in vitro techniques. Trends in Sciences, 19(14), 5096. doi: 10.48048/tis.2022.5096.

Fatawy, R. M. (2016). Produksi gas metana dari substrat Batubara lignit oleh mikroorganisme cairan rumen dengan metode scale up dalam fermentor 1000 l (Undergraduate thesis). UIN Syarif Hidayatullah Jakarta, Indonesia.

Food and Agriculture Organization (FAO). (2022). Livestock and enteric methane. Retrieved from https://www.fao.org/in-action/enteric-methane/background/en

Febriyani, W. (2019). Pengaruh amoniasi dan fermentasi menggunakan Aspergillus niger pada kulit kopi terhadap vfa total dan nh3 cairan rumen sapi secara in vitro (Undergraduate thesis). Universitas Lampung, Bandar Lampung, Indonesia.

Firkins, J. L., Yu, Z., Park, T., Plank, J. E. (2020). Extending Burk dehority's perspectives on the role of ciliate protozoa in the rumen. Front Microbiol, 11, 123.

General Laboratory Procedures. (1966). Department of Dairy Sciences. Madison: University of Wisconsin.

Getabalew, M., Alemneh, T., & Akeberegn, D. (2019). Methane production in ruminant animals: Implication for their impact on climate change. Concepts of Dairy & Veterinary Sciences, 2(4), 204-211.

Gustiar, F., Suwignyo, R. A., Suheryanto., & Munandar. (2014). Reduksi gas metan (ch4) dengan meningkatan komposisi konsentrat dalam pakan ternak sapi. Jurnal Peternakan Sriwijaya, 3(1), 14-24.

Hapsari, N. S., Harjanti, D. W., & Muktiani, A. (2018). Fermentabilitas pakan dengan imbuhan ekstrak daun babadotan (Ageratum conyzoides) dan jahe (Zingiber officinale) pada sapi perah secara in vitro. Jurnal Agripet, 18(1), 1-9.

Hassan, A., Akmal, Z. & Khan, N. (2020). The phytochemical screening and antioxidants potential of Schoenoplectus triqueter L. Palla. Journal of Chemistry, 2020, 3865139. doi: 10.1155/2020/3865139.

Hidayah, N. (2016). Pemanfaatan senyawa metabolit sekunder tanaman (tanin dan saponin) dalam mengurangi emisi metan ternak ruminansia. Jurnal Sain Peternakan Indonesia, 11(2), 89-98.

Jayanegara, A., Tjakradidjaja, A. S., & Sutardi, T. (2006). Fermentabilitas dan kecernaan in vitro ransum limbah agroindustri yang disuplementasi kromium anorganik dan organik. Media Peternakan, 29(2), 54-62.

Jayanegara, A., Makkar, H. P. S., & Becker, K. (2009). Emisi metana dan fermentasi rumen in vitro ransum hay yang mengandung tannin murni pada konsentrasi rendah. Media Peternakan, 32(3), 184-194.

Karsa, M. H. (2016). Kualitas produk fermentasi cairan rumen pada domba yang diberi pakan silase sorghum (Sorghum bicolor L.) yang disuplementasi probiotik bios k2 dengan metode rusitec (Undergraduate thesis). UIN Syarif Hidayatullah Jakarta, Indonesia.

Krishnamoorthy, U. (2001). RCA training workshop on in vitro techniques for feed evaluation. Jakarta: The International Atomic Energy Agency.

Krisnawan, N. (2015). Efek asosiatif senyawa tanin dan saponin dengan pakan berbasis jerami padi amoniasi terhadap emisi gas metana dan fermentasi rumen secara in vitro (Thesis master’s). IPB, Bogor, Indonesia.

Makkar, H.P. S. (2003). Effects and fate of tannins in ruminant animals, adaptation to tannins-rich feed. Small Ruminant Research, 49(3), 241-256.

Mao, S., Huo, W., Liu, J., Zhang R., Zhu, W. (2017). Invitro effects of sodium bicarbonate buffer on rumen fermentation, levels of lipopolysaccharide and biogenic amine, and composition of rumen microbiota. Journal of the Science of Food and Agriculture, 97(4), 1276-1285.

Masruroh, S., Prayitno, C. H., & Suwarno. (2013). Populasi protozoa dan produksi gas total dari rumen kambing perah yang pakannya disuplementasi ekstrak herbal secara in vitro. Jurnal Ilmiah Peternakan, 1(2), 420-429.

Menke, K. H., Raab, L., Salewski, A., Steingass, H., Fritz, D., & Schneider, W. (1979). The estimation of the digestibility and metabolizable energy content of ruminant feeding stuffs from the gas production when they are incubated with rumen liquor in vitro. Journal of Agricultural Science, 93, 217-222.

Nawab, A., Li, G., Nawab, Y., Zhao, Y., Xiao, M., Tang, S., & Sun, C. (2020). The potential effect of dietary tannins on enteric methane emission and ruminant production, as an alternative to antibiotic feed additives-a review. Annals of Animal Science, 20(2), 355-388.

Nuswantara, L. K. (2009, May 20). Parameter fermentasi rumen pada kerbau yang diberi pakan tunggal glirisidia, jerami jagung dan kaliandra. Paper presented at the Kebangkitan Peternakan Conference, Semarang, Indonesia. Retrieved from http://eprints.undip.ac.id/3816/1/aPR27-(75)_Limbang_KN-undip-seting.pdf.

Opio C, Gerber P, Mottet A, Falcucci A, Tempio G, MacLeod M, et al. Greenhouse gas emissions from ruminant supply chains – A global life cycle assessment [document on the Internet]. c2013 [cited 2020 Jan 30]. Available from: http://www.fao.org/3/i3461e/i3461e.pdf

Opio C, Gerber P, Mottet A, Falcucci A, Tempio G, MacLeod M, et al. Greenhouse gas emissions from ruminant supply chains – A global life cycle assessment [document on the Internet]. c2013 [cited 2020 Jan 30]. Available from: http://www.fao.org/3/i3461e/i3461e.pdf

Opio, C., Gerber, P., Mottet, A., Falcucci, A., Tempio, G., MacLeod, M., … Steinfeld, H. 2013. Greenhouse gas emissions from ruminant supply chains – a global life cycle assessment. Rome: Food and Agriculture Organization of the United Nations (FAO).

Plummer, D. T. (1971). An introduction to biochemistry. New York: Tatta McGraw-Hill Publishing company.

Preston, T. R., & Leng, R. A. (1987). Matching ruminant production systems with available resources in the tropic and sub-tropic. Queensland: International color production. Stanthorpe.

Purbowati, E., Rianto, E., Dilaga, W. S., Lestari, C. M. S., & Adiwinarti, R. (2014). Karakteristik cairan rumen, jenis dan jumlah mikrobia dalam rumen sapi Jawa dan peranakan ongole. Buletin Peternakan, 38(1), 21-26.

Ranja, E. P., Sudarma, I, M. A., Hambakodu, M. (2020). Nilai VFA dan NH3 rumput alam padang penggembalaan Kecamatan Haharu Kabupaten Sumba Tidur. Jurnal Ilmu Peternakan Terapan, 5(1), 8-12.

Research Centre for Medicinal and Aromatic Plants. (2011). Limbah serai wangi potensial sebagai pakan ternak. Warta Penelitian dan Pengembangan Pertanian, 33(6), 10-12.

Romli, S. D. (2022). Produksi gas total silase pakan komplit secara in vitro menggunakan tanin chestnut sebagai aditif silase (Undergraduate thesis). UIN Sultan Syarif Kasim, Riau, Indonesia.

Sari, A. F., Manguwardoyo, W., & Sugoro, I. (2017). Degradation of waste and fresh citronella (Cymbopogon nardus L) using in sacco method in rumen fistulated buffalo. Proceedings of the Seminar Nasional Teknologi Peternakan dan Veteriner, West Java, Indonesia. Retrieved from https://repository.pertanian.go.id/server/api/core/bitstreams/f5af7cbb-2fe7-47df-a0b7-f1e782d4ca65/content.

Scholtz, M. M., Neser, F. W. C., Makgahlela, M. L. (2020). A balanced perspective on the importance of extensive ruminant production for human nutrition and livelihoods and its contribution to greenhouse gas emissions. South African Journal of Science, 116(9-10, 1-3.

Sugoro, I., & Yunianto, I. (2006). Pertumbuhan protozoa dalam cairan rumen kerbau yang disuplementasi tannin secara in vitro. Jurnal Ilmiah Aplikasi Isotop dan Radiasi, 2(2), 48-57.

Terranova, M., Kreuzer, M., Braun, U., Schwarm, A. (2018). In vitro screening of temperate climate forages from a variety of woody plants for their potential to mitigate ruminal methane and ammonia formation. The Journal Agricultural Science, 156(7) (2018), 929-941. doi: 10.1017/S0021859618000989.

Utiah, W., Paputungan, U., & Tangkau, L. M. S. (2021). Analisis faktor konsentrat pakan komersil berbeda terhadap sifat-sifat produksi ayam ras petelur, Zootec, 41(2), 525-533.

Wahyono, T. (2015). Evaluasi biologis ransum kerbau yang mengandung sorgum dengan pendekatan in sacco, in vitro dan rusitec (Master’s thesis). IPB, Bogor, Indonesia.




DOI: https://doi.org/10.15408/kauniyah.v18i2.40935

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