Keragaman Serbuk Sari Pada Tumbuhan Legum di Kampus IPB, Dramaga, Bogor
Abstract
Abstrak
Morfologi serbuk sari dapat dipakai untuk identifikasi tumbuhan, sumber bukti taksonomi, dan diterapkan dalam banyak disiplin ilmu. Penelitian ini bertujuan untuk menggambarkan ciri morfologi serbuk sari yang berasal dari tumbuhan legum. Sampel serbuk sari diperoleh dari bunga yang dikoleksi di area kampus. Koleksi bunga dilakukan dengan metode jelajah di kawasan kampus IPB, Dramaga. Sampel serbuk sari yang akan diamati dibuat dengan metode asetolisis. Pengukuran morfologi polen dihitung berdasarkan sumbu polar dan ekuatorial dalam skala mikrometer. Ciri morfologi serbuk sari yang diteliti meliputi bentuk, unit serbuk sari, tipe aperture, ukuran, indeks P/E, dan ornamentasi eksin. Serbuk sari diamati dengan mikroskop majemuk Olympus (CX-33) yang dilengkapi kamera indomikro. Hasil penelitian menunjukkan bahwa bentuk serbuk sari pada legum ada dua macam yaitu circular dan circular oval. Ukuran serbuk sari beragam dari sangat kecil sampai sangat besar. Serbuk sari memiliki indeks P/E > 1,00. Tipe aperture meliputi monoporate, triporate, tetraporate, tricolpate, dan tricolporate. Ornamentasi eksin yang dijumpai berupa scabrate, reticulate, dan psilate. Serbuk sari yang diamati pada penelitian ini menggambarkan morfologi yang khas sehingga dapat dijadikan karakter tambahan dalam mengidentifikasi tumbuhan legum.
Abstract
Pollen morphology can be usefull for plant identification, sources of taxonomic evidence, and apply in many disciplines. This study aimed to describe the morphological characteristics of pollen from legume plants. Pollen samples were obtained from flowers collected in the campus area. The collection of flowers was carried out using the roaming method in the area of the IPB campus, Dramaga. The pollen sample to be observed was made by the acetolysis method. Pollen morphology measurements were calculated based on the polar and equatorial axes on the micrometer scale. The morphological characteristics of the pollen studied included pollen shape, pollen unit, aperture type, size, P/E index, and exine ornamentation. Pollen grains were observed with an Olympus compound microscope (CX-33) equipped with an indomicro camera. The results showed that there are two forms of pollen grains, namely circular and circular oval. Pollen sizes vary from very small to very large. Pollen has a P/E index > 1.00. The aperture types include monoporate, triporate, tetraporate, tricolpate, and tricolporate. The exine ornamentation found is scabrate, reticulate, and psilate. The pollen observed in this study shows a distinctive morphology so that it can be used as an additional character in identifying legume plant.
Keywords
Full Text:
PDFReferences
Ahmad, F., Anwar, F., & Hira, S. (2016). Review on medicinal importance of Fabaceae family. Pharmacology online, 3, 151-156.
Ajipe, J. O., & Adebayo, M. B. (2018). The significance of palynology in socio-economic development in Nigeria. Aims Research Journal, 4(1), 51-58.
Alege, G. O., Abu, N. E., & Sunday, C. E. (2014). Seed protein electrophoresis of some members of the family Fabaceae. African Journal of Biotechnology, 13(36), 3730-3735. doi: 10.5897/AJB2014.13715.
Aye, T. T., & Lin, S. S. (2020). Taxonomy and pollen morphology of nine species in Fabaceae. Journal of the Myanmar Academy of Arts and Science, 18(4), 301-313.
Bahadur, S., Taj, S., Long, W., & Hanif, U. (2022). Pollen morphological peculiarities of selected Mimosoideae taxa of Hainan Island and their taxonomic relevance. Agronomy, 12(5), 2-13. doi: 10.3390/agronomy12051122.
Bastl, K., Berger, U., & Kmenta, M. (2017). Evaluation of pollen apps forecasts: The need for quality control in an ehealth service. Journal of Medical Internet Research, 19(5), 1-8. doi: 10.2196%2Fjmir.7426.
Buril, M. T., Alves, M., & Santos, F. A. R. (2011). Tipifi cação polínica em Leguminosae de uma área prioritária para conservação da Caatinga: Caesalpinioideae e Papilionoideae. Acta Botanica Brasilica, 25(3), 699-712. doi: 10.1590/S0102-33062011000300023.
Devi, R. K., Rajesh, N. V., & Kumari, R. G. (2013). Palynological studies on selected eight species of Acacia Willd in South India. Romanian Journal of Biology, 58(1), 69-77.
Domingues, H. A., Correa, A. M. S., Queiroz, R. T., & Bitar N. A. B. (2018). Pollen morphology of some Fabaceae from Patos de Minas, Minas Gerais State, Brazil. Hoehnea, 45(1), 103-114. doi: 10.1590/2236-8906-54/2017.
Erdtman, G. (1952). Pollen morphology and plant taxonomy Angiospermae (an introduction to palinology I). Stockholm: Chronica Botanica.
Erdtman, G. (1954). An introduction to pollen analysis. Waltham: Chronica Botanica.
Erdtman, G. (1969). Handbook of palynology, morphology, taxonomy, ecology. An introduction to study of pollen grains and spores. New York: Hafner Publishing Co.
Faegri, K., & Iversen, J. (1989). Textbook of pollen analysis. New York: Hafner Press.
Fitri, R., Des, M., & Handayani, D. (2016). Morfologi serbuk sari pada beberapa variasi warna mahkota bunga Caesalpinia pulcherrima (L.) Swartz. Eksakta, 2, 38-43.
Ghosh, S., & Mandal, S. (2016). Pollen atlas of Santiniketan, West Bengal, with reference to aeropalynology. International Journal of Current Microbiology and Applied Sciences, 5(6), 983-1000. doi: 10.20546/ijcmas.2016.505.104.
Gosling, W. D., Miller, C. S., & Livingstone, D. A. (2013). Atlas of the tropical West African pollen flora. Review of Palaeobotany and Palynology, 199, 1-135. doi: 10.1016/j.revpalbo.2013.01.003.
Halbritter, H., Ulrich, S., Grimsson, F., Weber, M., Zetter, R., Hesse, M., … Frosch-Radivo, A. (2018). Pollen morphology and ultrastructure. Vienna: Springer.
Jayadi, L. Z., & Susandarini, R. (2020). Melissopalynological analysis of honey produced by two species of stingless bees in Lombok Island, Indonesia. Nusantara Bioscience, 12(2), 97-108. doi: 10.13057/nusbiosci/n120203.
Jones, G. D., & Jones, S. D. (2001). The uses of pollen and its implication for entomology. Neotropical Entomology, 30(3), 341-350. doi: 10.1590/S1519-566X2001000300001.
Jumah, A. (1996). Studies on the morphology of pollen grains of the Leguminosae-the Mimosoideae. doi: 10.4314/gjs.v36i1.47978.
Kailas, J. G., Ramakrishna, H., & Prabhakar, R. (2014). Palynodiversity of arborescent plants of Caesalpiniaceae family of Karimnagar district, Telangana State, India. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 5(6), 349-353.
Kapp, R. O. (1969). How to know pollen and spores. Dubuque (US): W.C. Brown Co. Publisher.
Khan, I., Akhtar, N., & Khan, S. A. (2019). Palynological investigation of some selected species of family Fabaceae from Pakistan, using light and scanning electron microscopy techniques. Microscopy Research and Technique, 83(3), 1-9. doi: 10.1002/jemt.23563.
Kim, S. S., & Douglas, C. J. (2013). Sporopollenin monomer biosynthesis in arabidopsis. Journal of Plant Biology, 56, 1-6. doi: 10.1007/s12374-012-0385-3.
Kumari, M., Sankhla, M. S., Nandan, M., Sharma, K., & Kumar, R. (2017). Role of forensic palynology in crime investigation. International Journal of Social Relevance and Concern, 5(3), 1-13.
Lavia, G. I., Ortiz, A. M., Robledo, G., Fernandez, A., & Seijo, G. (2011). Origin of triploid Arachis pintoi (Leguminosae) by autopolyploidy evidenced by fish and meiotic behavior. Annals of Botany, 108(1), 103-111. doi: 10.1093/aob/mcr108.
Moore, P. D., & Webb, J. A. (1978). An illustrated guide to pollen analysis. London: John Wiley & Sons
Mustari, A. H. (2020). Biodiversitas di Kampus IPB University: mamalia, burung, amfibi, reptil, kupu-kupu, dan tumbuhan. Bogor: IPB Press.
Raffiudin, R., Ariyanti, N. S., Aprilianingrum, I., Anwar, H., Shullia, N.I., Bening, S., … Purnobasuki, H. (2022). Flight activity and pollen resources of Apis nigrocincta and Apis cerana in Central Sulawesi, Indonesia. Agriculture and Natural Resources, 56, 463-472. doi: 10.34044/j.anres.2022.56.3.03.
Raj, P. R., & Reddy, A. V. B. (2019). Pollen morphology study of Leguminosae family from Manchippa Reserve Forest, Nizamabad district, Telangana. Journal of Tree Sciences, 38(2), 1-18. doi: 10.5958/2455-7129.2019.00019.0.
Redden, K. M., Herendeen, P. S., Kenneth, J., Wurdack, K. J., & Bruneau, A. (2010). Phylogenetic relationships of the Northeastern South American brownea clade of tribe Detarieae (Leguminosae: Caesalpinioideae) based on morphology and molecular data. Systematic Botany, 35(3), 524-533. doi: 10.1600/036364410792495863.
Rugayah, A., Retnowati, F. I., Windadri, A., & Hidayat. (2004). Pengumpulan data taksonomi. In Rugayah, E. A. Widjaja, Praptiwi (Eds.). Pedoman pengumpulan data keanekaragaman flora. Bogor: Pusat Penelitian Biologi-Lembaga Ilmu Pengetahuan Indonesia.
Simpson, M. G. (2010). Diversity and classification of flowering plants: Eudicots. Amsterdam: Elsevier Science.
Sopaladawan, P. N., Namwong, L., & Wongnaikod, S. (2019). Food plant species from pollen load of honey bee (Apis mellifera) in Nong Khai Province, Thailand. International Journal of Sciences, 16(1), 36-45.
Sopaladawan, P. N. (2020). Pollen resources for the honey bee, Apis mellifera in Roi-Et Province, Thailand. Journal of Food Health and Bioenvironmental Science, 13(2), 10-16.
Taia, W. K., & Zayed, A. A. H. (2021). Road tree pollen grain contents and effect on the immune system. Quantum Journal of Medical and Health Sciences, 1(4), 34-50.
Tantawy, M. E., Khalifa, S. F., Hamed, K. A., & Elazab, H. M. (2005). Palynological study on some taxa of Mimosoideae (Leguminosae). International Journal of Agriculture and Biology, 7(6), 858-868.
Tschudy, R. H., & Scott, R. A. (1969). Aspect of palynology. New Jersey (US): John Willey.
Umdale, S. D., Aitawade, M. M., Gaikwad, N. B., Madhavana, L., Yadav, S. R., Raos, S. R., & Bhati, K. V. (2017). Pollen morphology of Asian Vigna species (genus Vigna; subgenus Ceratotropis) from India and its taxonomic implications. Turkish Journal of Botany, 41, 75-81. doi: 10.3906/bot-1603-31.
Wu, M. J., & Huang, T. C. (1995). A palynological study of the genus Indigofera (Leguminosae) in Taiwan. Grana, 34, 160-181. doi: 10.1080/00173139509429040.
DOI: https://doi.org/10.15408/kauniyah.v17i2.29846 Abstract - 0 PDF - 0
Refbacks
- There are currently no refbacks.
This work is licensed under a CC-BY- SA.
Indexed By:
   