Respon IAA dan BAP Terhadap Multiplikasi In Vitro Tunas Tanaman Krisan Varietas Suciono (Chrysanthemum indicum L)
Abstract
Abstrak
Tanaman krisan varietas Suciono merupakan tanaman hias yang potensial dikembangkan dan diminati oleh penduduk dunia, yang ditunjukkan dengan permintaan pasar terhadap tanaman krisan meningkat sepanjang tahun. Hal ini menyebabkan perbanyakan krisan secara in vitro sangat penting untuk dilakukan. Penelitian ini bertujuan untuk mengetahui pengaruh Indole Acetic Acid (IAA) dan Benzly Amino Purine (BAP) terhadap multiplikasi tunas krisan varietas Suciono. Desain penelitian ini menggunakan Rancangan Acak Lengkap dengan satu faktor yakni kombinasi hormon IAA dan BAP yang 9 kombinasi, yakni 0 mg/L + 1,5 mg/L; 0 mg/L + 2 mg/L; 0 mg/L + 2,5 mg/L; 0,5 mg/L + 1,5 mg/L; 0,5 mg/L + 2 mg/L; 0,5 mg/L + 2,5 mg/L; 1 mg/L + 1,5 mg/L; 1 mg/L + 2 mg/L; dan 1 mg/L + 2,5 mg/L dimana setiap kombinasi diulang sebanyak 3 kali. Variabel yang diamati meliputi analisis histologis kalus, waktu muncul tunas, jumlah tunas, tinggi tunas, dan jumlah daun. Hasil analisis ragam atau ANOVA menunjukkan bahwa kombinasi IAA 0 mg/L dan BAP 2 mg/L merupakan kombinasi konsentrasi terbaik untuk multiplikasi tunas. Penambahan BAP 2 mg/L mampu menghasilkan persentase muncul tunas. 100%, jumlah tunas 3 per-eksplan, tinggi tunas 7 cm, dan jumlah daun sebanyak 16 helai.
Abstract
The variety Suciono of chrysanthemum is a potential ornamental plant that is in demand by the world's population, as indicated by the increasing market demand for chrysanthemum plants throughout the year. This has led to the importance of in vitro propagation of chrysanthemum. The aim of the this study is to determine the effect of Indole Acetic Acid (IAA) and Benzly Amino Purine (BAP) on the multiplication of chrysanthemum shoots of the Suciono variety. The design of this study used nodus explants, treated with a combination of IAA hormones and BAP. It is make 9 combinations, and then the combinations of hormones is 0 mg/L + 1,5 mg/L; 0 mg/L + 2 mg/L; 0 mg/L + 2,5 mg/L; 0,5 mg/L + 1,5 mg/L; 0,5 mg/L + 2 mg/L; 0,5 mg/L + 2,5 mg/L; 1 mg/L + 1,5 mg/L; 1 mg/L + 2 mg/L; and 1 mg/L + 2,5 mg/L. The observed variables included histological analysis of callus, time of shoot emergence, number of shoots, shoots height, and number of leaves. The results of the analysis showed that the treatment with 0 mg/L IAA and 2 mg/L BAP was the best concentration for shoots multiplication. The addition of 2 mg/L BAP was able to produce a percentage of shoots emergence of 100%, 3 shoots per explant, shoots height of 7 cm and 16 leaves.
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DOI: https://doi.org/10.15408/kauniyah.v16i2.1.27417 Abstract - 0 PDF - 0
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