Pengaruh Pemberian Jus Pare (Momordica charantia L.) Terhadap Kualitas Sperma dan Histologi Testis Tikus Wistar
Abstract
Abstrak
Buah pare merupakan salah satu kandidat agen kontrasepsi karena memiliki beberapa senyawa seperti flavonoid, triterpenoid, steroid, charantin, dan momordicin yang memiliki peran sebagai agen antispermatogenik. Penelitian bertujuan untuk mengetahui pengaruh pemberian jus pare (Momordica charantia L.) terhadap kualitas sperma dan histologi testis tikus Wistar (Rattus norvegicus Berkenhout, 1769). Penelitian menggunakan 20 ekor tikus Wistar jantan, dibagi menjadi 4 perlakuan yang terdiri dari kelompok pemberian akuades (K), kelompok pemberian jus pare konsentrasi 25% (P1), 50% (P2), dan 75% (P3) yang dilakukan selama 49 hari menggunakan sonde lambung 1 mL. Pada hari ke-50 tikus dibedah untuk diambil cauda epididymis dan testis. Cauda epididymis dilarutkan ke dalam Phospate Buffer Saline (PBS) untuk pengamatan kualitas sperma yang terdiri dari motilitas, jumlah, viabilitas, dan morfologi sperma. Testis dibuat sediaan histologi dengan metode parafin (pewarnaan Hematoxylin-Eosin). Seluruh parameter dianalisis menggunakan uji One-Way Anova dan dilanjutkan Duncan test (P <0,05). Hasil menunjukkan terdapat penurunan motilitas, viabilitas, dan jumlah sperma pada konsentrasi 50% dibandingkan dengan kontrol (P <0,05), namun tidak terdapat perbedaan pada morfologi sperma (P >0,05). Terdapat penurunan jumlah sel spermatogonium, spermatosit, dan spermatozoa pada konsentrasi 25% dibandikan dengan kontrol (P <0,05), namun tidak terlihat penurunan pada sel spermatid dan index spermatogenesis (P >0,05). Jus pare dapat menurunkan sebagian besar parameter kualitas sperma sehingga berpotensi sebagai antispermatogenik.
Abstract
Bitter melon fruit is one of the candidates for contraceptive agents because it contains several compounds such as flavonoids, triterpenoids, steroids, charantin, and momordicin which have a role as antispermatogenic agents. The research aims to determine the effect of giving bitter melon juice (Momordica charantia L.) on sperm quality and testicular histology of Wistar rats (Rattus norvegicus Berkenhout, 1769). The study used 20 male Wistar rats, divided into 4 treatments consisting of groups given distilled water (K), groups given bitter melon juice with concentrations of 25% (P1), 50% (P2), and 75% (P3) which were carried out for 49 days. using a 1 mL gastric probe. On the 50th day, mice were dissected to remove the cauda epididymis and testes. The cauda epididymis is dissolved in Phosphate Buffer Saline (PBS) to measure sperm quality consisting of sperm motility, number, viability and morphology. Testes were made into histological preparations using the paraffin method (Hematoxylin-Eosin staining). All parameters were analyzed using the One-Way Anova test and continued with the Duncan test (P<0.05). The results showed that there was a decrease in sperm motility, viability and number at a concentration of 50% compared to the control (P<0.05), but there was no difference in sperm morphology (P>0.05). There was a decrease in the number of spermatogonium cells, spermatocytes and spermatozoa at a concentration of 25% compared to the control (P<0.05), but there was no visible decrease in spermatid cells and spermatogenesis index (P>0.05). Bitter melon juice can reduce most sperm quality parameters so it has the potential to be antispermatogenic.
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Badan Pusat Statistik (BPS). (2018). Inilah proyeksi jumlah penduduk Indonesia 2020. (2020, September 26) Retrieved from http://databoks.katadata.co.id/datapublish/2020/01/02/inilah-proyeksi-jumlah-penduduk-indonesia-2020.
Badan Kependudukan dan Keluarga Berencana Nasional (BKKBN). (2013). Profil kependudukan dan pembangunan di Indonesia tahun 2013. Jakarta: BKKBN.
Cholifah, S., Arsyad., & Salni. (2014). Pengaruh pemberian ekstrak pare (Momordica charantia, L.) terhadap struktur histologi testis dan epididimis tikus jantan (Rattus norvegicus) Sparaque Dawley. Majalah Kedokteran Sriwijaya, 46(2), 149-157.
Delfita, R. (2014). Potensi antifertilitas ekstrak teh hitam pada mencit (Mus musculus L.) Jantan. Jurnal Sainstek, 6(2), 181-188. doi: 10.31958/js.v6i2.117.
Dewanti, E., Viviandhari, D., Lonica, N., & Isnarningtyas, S. M. (2020). Efek antifertilitas dari ekstrak daun pepaya (Carica papaya L.) pada tikus putih jantan galur Sprague Dawley. Jurnal Jamu Indonesia, 5(1), 09-15.
Fifendy, M., & Indriati, G. (2018). Test of fruit extract pare (Momordica charanthia L.) to quality of ejaculated spermatozoa mice (Mus musculus L.). IOP Conference Series: Materials Science and Engineering, 335, 012136.
Harlis, W. O., Malik, N., & Nelpiani. (2015). Efek pemberian sari buah paria (Momordica charantia, L.) terhadap morfologi spermatozoa epididimis mencit (Mus musculus, L.).Biowallacea, 2(1), 196-203. doi: 10.33772/biowallacea.v2i1.538.
Hendra, R. (2014). Pemantapan partisipasi pria dalam program keluarga berencana. Jurnal Parallela, (1), 71-78.
Januarti, I. B., Latifah, F., & Fatmawati, N. F. (2021). Efek ekstrak bawang putih tunggal (Allium sativum var. Solo) terhadap sel leydig dan sel sertoli tikus. Jurnal Farmasi Sains dan Praktis, 7(1), 1-6.
Jerald, E., Pandey, A., Bigoniya, P., & Singh, S. B. (2012). Antifertility activity of Momordica charantia descourt pulp and seed hydroalcoholic extract. Journal of Applied Pharmacy, 03(04), 682-696.
Joshi, S. C., Sharma, A., & Chaturvedi, M. (2011). Antifertility potential of some medicinal plants in males: An overview. International Journal of Pharmacy and Pharmaceutical Sciences, 3(5), 204-217.
Kaspul. (2016). Pengaruh buah terong pipit (Solanom torvum Sw) terhadap spermatogenesis tikus putih (Rattus norvegicus L.). Sains dan Terapan Kimia, 10(1), 99-104. doi: 10.20527/jstk.v10i1.3158.
Mada, S. B., Garba A., Mohammed, H. A. A., Muhammad, A., & Abbas, O. (2013). Antimicrobial activity and phytochemical screening of aqueous and ethanol extracts of Momordica charantia L. leaves. Journal of Medicinal Plants Research, 7(10), 579-586, doi: 10.5897/JMPR12.1161.
Nita, S., Setiawan, A., Inggarsih, R., Tantri, U., & Hardiana, M. (2020). Papaya (Carica papaya L.) seed extract as male contraception via decreasing the quality of rat’s (Rattus norvegicus) sperm. Bioscientia Medicine : Journal of Biomedicine and Translational Research, 4(1), 19-28.
Obiandu, C., & Obiandu, A. C. (2020). Fertility index and percentage fertility of Momordica charantia treated male wistar rats. Saudi Journal of Biomedical Research, 5(2), 14-16. doi: 10.36348/sjbr.2020.v05i02.001.
Oguejiofor, C. F., Eke, I. G., & Anya, K. O.(2020). Antifertility effects of Azadirachta indica methanol seed extract on canine spermatozoa in vitro. Asian Pacific Journal of Reproduction, 9(3), 135-141. doi: 10.4103/2305-0500.284271.
Oliveira, M. S., Costa, W. A., Bezerra, F. W. W., Araújo, M. E., Ferreira, G. C., & Junior, R. N. C. (2018). Phytochemical profile and biological activities of Momordica charantia L. (Cucurbitaceae): A review. African Journal of Biotechnology, 17(27), 829-846. doi: 10.5897/AJB2017.16374.
Parhizkar, S., Maryam, J. Y., & Mohammad, A. D. (2013). Effect of Phaleria macrocarpa on sperm characteristics in adult rats. Advanced Pharmaceutical Bulletin, 3(2), 345-352. doi: 10.5681/apb.2013.056.
Rinendyaputri, R., & Nikmah, U. A. (2020, October 28). Potensi growth factor (gf) dari sel punca mesenkim untuk pematangan sel telur secara in vitro. Paper presented at the Prosiding Seminar Nasional SIMBIOSIS V, Madiun, Indonesia. Retrieved from http://prosiding.unipma.ac.id/index.php/simbiosis/article/viewFile/1768/1509.
Setiawan, H., & Yunianto, I. (2015). Aktivitas antispermatogenik ekstrak etanol daun jambu mete (Anacardium Occidentale L.) terhadap mencit (Mus musculus, L.) sebagai materi pembelajaran siswa sma kelas xi ipa untuk mencapai kd 3.12 kurikulum 2013. JUPEMASI-PBIO, 1(2), 2407-1269.
Setiawan H., & Yunianto, I. (2016, August 27). Pengaruh ekstrak etanol daun jambu mete (Anacardium Occidentale L.) terhadap perkembangan sel spermatogenik mencit (Mus musculus L.). Paper presented at the Prosiding Symbion (Symposium on Biology Education), Prodi Pendidikan Biologi, FKIP, Universitas Ahmad Dahlan, Indonesia. Retrieved from https://www.researchgate.net/profile/Haris-Setiawan-6/publication/337388214_PENGARUH_EKSTRAK_ETANOL_DAUN_JAMBU_METE_Anacardium_occidentale_L_TERHADAP_PERKEMBANGAN_SEL_SPERMATOGENIK_MENCIT_Mus_musculus_L/links/5dd4ef3c458515cd48ac1b82/PENGARUH-EKSTRAK-ETANOL-DAUN-JAMBU-METE-Anacardium-occidentale-L-TERHADAP-PERKEMBANGAN-SEL-SPERMATOGENIK-MENCIT-Mus-musculus-L.pdf.
Setiawan, H., Maliza, R., Maulana, S. A., & Hisbullah, M. I. (2020). The effect of coffee fruit extract on sperm characteristics and testicular of mice with ethanol-induced. Jurnal Biodjati, 5(2), 259-270. doi: 10.15575/biodjati.v5i2.9280.
Setiawan, H., Wulandari, S. W., & Agustina, E. D. (2021). Antispermatogenic activity of ethanolic leaves extract of calina papaya on seminiferous tubules wistar rats. Jurnal Kedokteran Hewan, 15(1), 21-26. doi: 10.21157/j.ked.hewan.v15i1.18435.
Setiawan, H., Wulandari, S. W., & Fachmi, M.N. (2022). Efek antispermatogenik ekstrak etanol daun pepaya calina terhadap kualitas sperma dan morfologi epididimis tikus wistar. Berita biologi, 20(3), 19-27. doi: 10.14203/beritabiologi.v21i1.4175.
Sharma, R. K., Goyal, A. K., & Bhat, R. A. (2013a). Antifertility activity of plants extracts on female reproduction: A review. International Journal of Pharmacy and Biological Sciences, 3(3), 493-514.
Sharma, P., Sharma, A., Agarwal, M., & Joshi, M. C. (2013b). A review on antifertility efficacy of plants in males. International Journal of Pharmacy and Biological Sciences, 4(4), 413-428. doi: 10.22376/ijpbs.
Singh A., & Teotia, U. V. S. (2017). Evaluation of effect of Momordica dioica extract on reproductive system of male and female rats. Biomedical & Pharmacology Journal, 10(3), 1419-1425. doi: 10.13005/bpj/1248.
Soliman, G. A., Rahman, R. A., Ogaly, H. A., Althurwi, H. N., Elsalam, R. M. A., Albaqami, F. F., & Kader, M. S. A. (2020). Momordica charantia extract protects against diabetes-related spermatogenic dysfunction in male rats: Molecular and biochemical study. Molecules, 25(22), 5255. doi: 10.3390/molecules25225255.
Susetyarini, E. (2012, December 18). Jumlah spermatogonia tikus putih yang diberi tanin daun beluntas (Pluchea indica) dengan berbagai waktu pengamatan. Paper presented at the Prosiding Seminar Nasional: Menuju Masyarakat Madani dan Lestari, Universitas Islam Indonesia, Yogyakarta, Indonesia. Retrieved from http://repository.polman-bandung.ac.id/file_publikasi/527650588_siti%20aminah_Pembangunan%20Perangkat%20Lunak%20Kurikulum.pdf.
Susilo, A. B., & Pratinaningsih, K. (2018). Pengaruh ekstrak etanol daun sambiloto terhadap jumlah dan motilitas spermatozoa mencit jantan. Jurnal Biodjati, 3(2), 2541-4208. doi: 10.15575/biodjati.v3i2.3505.
Tumkiratiwong, P., Ploypattarapinyo, R., Pongchairerk, U., & Thong-asa, W. (2014). Reproductive toxicity of Momordica charantia ethanol seed extracts in male rats. Iranian Journal of Reproductive Medicine, 12(10), 695-704.
Wulandari. (2016). Uji efektivitas antihiperglikemia kombinasi jus pare (Momordica charantia L) dan jus tomat (Solanum lycopersicum L) pada tikus wistar jantan dengan metode toleransi glukosa. Pharmaceutical Sciences and Research, 3(3), 2407-2354. doi: 10.7454/psr.v3i3.3269.
Wyrobek, A. J., & Bruce, W. R. (1975). Chemical induction of sperm abnormalities in mice. Proceedings of the National Academy of Sciences of the United States of America, 72(11), 4425-4429. doi: 10.1073/pnas.72.11.4425.
Yama, O. E., Duru, F. I., Oremosu, A. A., Norohna, C. C., & Okanlawon A. (2011) Suppressive effects of Momordica charantia on pituitary-testicular axis and sperm production in male Sprague-Dawley rats. International Journal of Medicine and Medical Sciences 3(12), 353-359. doi: 10.5897/IJMMS.9000230.
DOI: https://doi.org/10.15408/kauniyah.v16i2.1.23216 Abstract - 0 PDF - 0
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