Aktivitas Antimikroba Bakteri Endofit Daun Pegagan (Centella asiatica L.) Terhadap Propionibacterium acnes
Abstract
Abstrak
Tingginya angka kejadian jerawat dan meningkatnya resistensi terhadap antimikroba memerlukan alternatif pengobatan yang berasal dari bahan alam. Beberapa studi menunjukkan bahwa bakteri endofit tertentu memproduksi senyawa bioaktif yang memiliki efek bagi kesehatan, terutama bakteri endofit yang diisolasi dari tumbuhan obat. Pegagan (Centella asiatica L.) merupakan tumbuhan obat yang mempunyai banyak manfaat seperti untuk mengobati masalah kulit, menyembuhkan luka, serta menjadi agen antimikroba. Penelitian ini bertujuan untuk mengetahui aktivitas antimikroba isolat bakteri endofit daun pegagan terhadap Propionibacterium acnes. Pengujian aktivitas antimikroba dilakukan dengan metode difusi cakram melalui pengukuran diameter zona hambat. Bakteri endofit yang didapat dari penelitian sebelumnya dikarakterisasi berdasarkan ciri-ciri dari morfologi koloni, morfologi sel, dan aktivitas biokimia. Sembilan belas isolat bakteri endofit memiliki aktivitas antimikroba terhadap Propionibacterium acnes, ditandai dengan terbentuknya zona hambat berdiameter antara 11,46–25,34 mm. Dua isolat yang memiliki kemampuan aktivitas antimikroba yang paling besar, yaitu isolat nomor 3 dan 17 dengan hasil identifikasi termasuk ke dalam genus Aeromicrobium. Berdasarkan hasil penelitian dapat disimpulkan bahwa bakteri endofit dari daun pegagan (Centella asiatica (L.) berpotensi sebagai antimikroba terhadap bakteri P. acnes.
Abstract
The high prevalence of acne cases and the increasing resistance to antimicrobials requires alternative treatments that originate from natural ingredients. Several studies have shown that certain endophytic bacteria produce bioactive compounds that benefits human health, especially those whom isolated from medicinal plants. Gotu kola or pegagan (Centella asiatica L.) is one of medicinal plant that has many benefits such as treating skin problems, healing wounds, and also known for an antibacterial agent. The aim of the study was to determine the antimicrobial activity of the endophytic bacterial isolates of pegagan against Propionibacterium acnes. The antimicrobial activity test were carried out using the disc diffusion method by measuring the diameter of the inhibition zone within isolate. Endophytic bacteria obtained from previous studies were characterized based on the characteristics of colony morphology, cell morphology and biochemical activity. 19 isolates of endophytic bacteria showed antimicrobial activity against P. acnes, indicated by the formation of inhibition zones ranging from 11.46 to 25.34 mm. Two isolates that had the greatest antibacterial activity, namely isolates number 3 and 17 were identified and belongs to the genus Aeromicrobium. Based on the results of this study, it can be concluded that endophytic bacteria from pegagan (Centella asiatica L.) leaves has potential as an antibacterial agent against Propionibacterium acnes bacteria.
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DOI: https://doi.org/10.15408/kauniyah.v16i1.20276 Abstract - 0 PDF - 0
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