Potensi Bakteri Endofit Asal Tanaman Pisang Klutuk (Musa balbisiana Colla) Sebagai Pendukung Pertumbuhan Tanaman

Triastuti Rahayu, Yekti Asih Purwestri, Siti Subandiyah, Donny Widianto



Bakteri endofit yang terdapat di tanaman pisang Klutuk dan keterkaitannya dengan sifat ketahanan tanaman pisang Klutuk pada cekaman biotik dan abiotik belum dilaporkan dalam publikasi ilmiah. Sebanyak 93 isolat bakteri endofit telah diperoleh dari pisang Klutuk, tetapi belum diketahui kemampuannya sebagai pendukung pertumbuhan tanaman (PPT). Penelitian ini bertujuan untuk mengetahui karakter isolat-isolat bakteri endofit dari pisang Klutuk sebagai pendukung pertumbuhan tanaman. Kelompok bakteri Gram positif dan negatif ditentukan dengan metode pewarnaan Gram. Kemampuan memfiksasi nitrogen (N2), memproduksi asam indol asetat (AIA), dan antagonisme terhadap Fusarium oxysporum f. sp. cubense (Foc) diuji untuk mengetahui kemampuan isolat bakteri endofit sebagai pendukung pertumbuhan tanaman. Hasil penelitian menunjukkan bahwa 87,10% isolat bakteri endofit dari tanaman pisang Klutuk merupakan kelompok bakteri Gram negatif dan 82,80% (77 isolat bakteri) menunjukkan karakter tunggal atau ganda sebagai PPT. Di dalam kelompok isolat tersebut, terdapat berturut-turut 60, 38, dan 20 bakteri yang mampu memfiksasi N2, menghasilkan AIA, dan antagonisme terhadap Foc. Hasil pengujian ini menunjukkan bahwa bakteri endofit dari pisang Klutuk didominasi oleh bakteri kelompok Gram negatif yang memiliki kemampuan sebagai pendukung pertumbuhan tanaman.


The role of endophytic bacteria on the biotic and abiotic resistance of Klutuk banana plants has never been reported. A total of 93 endophytic bacterial isolates were obtained from Klutuk banana plants in a previous study, but their potency as Plant Growth Promoting Bacteria (PGPB) is not elucidated. This study aims to characterize those 93 endophytic bacterial isolates. Gram staining was performed to differentiate between Gram-positive and negative bacteria among the isolates. The ability to fix nitrogen (N2), produce indole acetic acid (IAA) and antagonize Fusarium oxysporum f. sp. cubense (Foc) were also examined to determine their potency as PGPB. The results showed that 87.1% of the endophytic bacterial isolates were Gram-negative bacteria and 83.87% (78 bacterial isolates) had single or multiple traits of PGPB. Among the isolates, 60, 38, and 20 bacteria were able to fix N2, produce IAA, and antagonize Foc, respectively. The results indicated that the endophytic bacteria inhabiting Klutuk banana plant are dominated by Gram-negative PGPB.


Bakteri endofit; Pendukung pertumbuhan tanaman (PPT); Pisang Klutuk; Endophytic bacteria; Klutuk banana; Plant Growth Promoting (PGP)

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