Variasi Struktur Anatomi Daun Beberapa Jenis Alpinia Roxb. di Malesia

Eka Setiawan, Miftahudin Miftahudin, Marlina Ardiyani, Tatik Chikmawati



Ketidaksesuaian sistem klasifikasi Alpinia berdasarkan ciri morfologi dengan ciri molekuler menyebabkan sistem klasifikasi pada marga ini masih bermasalah. Data tambahan dari anatomi Alpinia diperlukan untuk mendukung sistem klasifikasi pada marga ini. Sayatan paradermal adaksial dan abaksial dari daun tujuh jenis Alpinia diamati. Sampel diambil dari koleksi Kebun Raya Bogor (KRB) yang mewakili jenis A. galanga, A. mutica, A. padacanca, A. macrocrista, A. malaccensis, A. pusilla, dan A. ligulata. Sebelas ciri Alpinia diamati seperti; ukuran stomata, jumlah stomata, indeks stomata, kerapatan stomata, tipe stomata, ukuran sel tetangga, jumlah sel tetangga, bentuk sel tetangga, ukuran epidermis, jumlah epidermis, dan bentuk epidermis. Hasil pengamatan menunjukkan bahwa beberapa ciri dapat digunakan untuk membedakan jenis Alpinia, seperti bentuk dan ukuran epidermis; ukuran, kerapatan dan indeks stomata; serta ukuran sel tetangga. Sementara ciri lainnya, seperti tipe stomata dan bentuk sel tetangga pada semua jenis Alpinia memiliki bentuk yang sama sehingga tidak dapat digunakan untuk membedakan jenis Alpinia.


The incompatibility of Alpinia classification system based on morphological and molecular character causes classification system of this genus is still problematic. Additional data from Alpinia anatomy are needed to support the classification system in this genus. Adaxial and abaxial sides paradermal incisions of seven Alpinia species were observed. The samples were taken from the Bogor Botanical Gardens (KRB) collection representing A. galanga, A. mutica, A. padacanca, A. macrocrista, A. malaccensis, A. pusilla, and A. ligulata. Eleven Alpinia characteristics were observed; stomatal size, number of stomata, stomatal index, stomatal density, stomatal type, neighboring cells size, number of neighboring cells, neighboring cell shape, epidermal cells size, number of epidermal cells, and epidermal cells shape. The results showed that several characteristics can be used to distinguish Alpinia species, such as the shape and size of epidermal cells; the size, density and index of stomata; and the size of neighboring cells. Several other characteristics, such as the type of stomata and shape of neighboring cells in all species of Alpinia have the same shape so the characters cannot be used to distinguish Alpinia species.



Alpinia; Anatomi; Paradermal; Alpinia; Anatomi; Paradermal; Zingiberaceae

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Almeida, E. B. Jr., Arau, ́jo J. S., Santos-Filho, F. S., & Zickel, C. S. (2013). Leaf morphology an anatomy of Manilkara Adans. (Sapotaceae) from northeastern Brazil. Plant Systematics and Evolution, 299, 1-9. doi: 10.1007/s00606-012-0697-2.

Arau, ́jo J. S., Azevedo, A. A., Silva, L. C., & Meira, R. M. S. A. (2010). Leaf anatomy as an additional taxonomy tool for 16 species of Malpighiaceae found in the Cerrado area Brazil. Plant Systematics and Evolution, 286, 117-131. doi: 10.1007/s00606-010-0268-3.

[ASEAN] The Association of Southeast Asian Nations. (2010). ASEAN Herbal and Medicinal Plants. Jakarta: ASEAN Press.

Benedict, J. C. 2015. A new technique to prepare hard fruits and seeds for anatomical studies. Applications in Plant Sciences, 3(10), 1-5. doi: 10.3732/apps.1500075.

Benedict, J. C., Smith, S. Y., Collinson, M. E., Leong–Škorničková, J., Specht, C. D., Marone, F., Xiao, X., & Parkinson, D. Y. (2015a). Seed morphology and anatomy and its utility in recognizing subfamilies and tribes of Zingiberaceae. American Journal of Botany, 102, 1814-1841. doi: 10.3732/ajb.1500300.

Benedict, J. C., Smith, S. Y., Collinson, M. E., Leong–Škorničková, J., Specht, C. D., Fife, J. L., Marone, F., Xiao, X., & Parkinson, D. Y. (2015b). Evolutionary significance of seed structure in Alpinioideae (Zingiberaceae). Botanical Journal of the Linnean Society, 178, 441-466. doi: 10.1111/boj.12257.

Buckley, T. N., & Mott, K. A. (2013). Modelling stomatal conductance in response to environmental factors. Plant, Cell & Environment, 36, 169-1699. doi: 10.1111/pce.12140.

Camargo, M. A. B., & Marenco, R.A. (2011). Density, size and distribution of stomata in 35 rainforest tree species in Central Amazonia. Acta Amazonica, 41(2), 205-212. doi: 10.1590/S0044-59672011000200004.

Carvalho, D. R. A., Torre, S., Kraniotis, D., Almeida, D. P. F., Heuvelink, E., & Carvalho, S. M. P. (2015). Elevated air movement enhances stomatal sensitivity to abscisic acid in leaves developed at high relative air humidity. Frontier in Plant Science, 6, 383. doi: 10.3389/fpls.2015.00383.

Dalvi, V. C., Meira R. M. S. A., Francino D. M. T., Silva L. C., & Azevedo A. A. (2014). Anatomical characteristics as taxonomic tools for the species of Curtia and Hockinia (Saccifolieae–Gentianaceae Juss.). Plant Systematics and Evolution, 300, 99-112. doi: 10.1007/s00606-013-0863-1.

De Boer, H., Newman, M., Poulsen, A. D., Droop, A. J., Fér, T., Hiền, L. T. T., Hlavatá, K., Lamxay, V., Richardson, J. E., Steffen, K., & Leong-Škorničková, J. (2018). Convergent morphology in Alpinieae (Zingiberaceae): Recircumscribing Amomum as a monophyletic genus. Taxon, 67(1), 6-36. doi: 10.12705/671.2.

Delgado, M. N., Azevedo, A. A., Valente, G. E., & Kasuya, M. C. M. (2011). Comparative anatomy of Calolisianthus species (Gentianaceae-Helieae) from Brazil: taxonomic aspects. Edinburgh Journal of Botany, 68(1), 139-155. doi: 10.1017/S0960428610000284.

Inceer, H., & Ozcan, M. (2011). Leaf anatomy as an additional taxonomy tool for 18 taxa of Matricaria L. and Tripleurospermum Sch.Bip. (Anthemideae-Asteraceae) in Turkey. Plant Systematics and Evolution. 296, 205-215. doi: 10.1007/s00606-011-0487-2.

Kajornjit, P., Saensouk, S., & Saensouk, P. (2018). Pollen morphology and leaf anatomy of genus Globba in Thailand. ScienceAsia, 44, 146-161. doi: 10.2306/scienceasia1513-1874.2018.44.146.

Kardiman, R., & Ræbild, A. (2018). Relationship between stomatal density, size and speed of opening in Sumatran rainforest species. Tree Physiology, 38(5), 696-705. doi: 10.1093/treephys/tpx149.

Kress, W. J., Newman, M., Poulsen, A. D., & Specht, C. J. (2007). An Analysis of generic circumscription in tribe Alpinieae (Alpinioideae: Zingiberaceae). Gardens Bulletin Singapore, 59(1&2), 113–124.

Leong-Škorničková, J., & Newman M. (2015). Gingers of Cambodia, Laos, and Vietnam. Singapore: Singapore Botanical Garden’s.

Marom, Z., Shtein, I., & Bar-On, B. (2017). Stomatal Opening: The role of cell-wall mechanical anisotropy and its analytical relations to the bio-composite characteristics. Frontiers in plant science, 8(2061):1-12. doi: 10.3389/fpls.2017.02061.

Matthew, S., Britto, J., & Thomas, S. (2014). Rhizome and leaf anatomical variations in Alpinia calcarata and Alpinia galanga. International Journal of Current Research, 6(5), 6761-6764.

Pompelli, M. F., Martins, S. C. V., Celin, E. F., Ventrella, M. C., & DaMatta, F. M. (2010). What is the influence of ordinary epidermal cells and stomata on the leaf plasticity of coffee plants grown under full-sun and shady conditions? Brazilian Journal of Biology, 70(4), 1083-1088. doi: 10.1590/s1519-69842010000500025.

Rudall, P. J., Chen E. D., & Cullen, E. (2017). Evolution and development of monocot stomata. American Journal of Botany, 104 (8), 1122-1141. doi: 10.3732/ajb.1700086.

Salasiah, M., & Meekiong K. (2018). Preliminary anatomical study on leaf surfaces of Bornean Zingiberaceae (Tribe Alpinieae) from North East Sarawak. Malaysian Applied Biology, 47(5), 289-293.

Sass, J. E. (1951). Botanical Microtechnique. Iowa: Iowa State College Pr.

Setyawan, A. D. (2001). Anatomi sistematik pada anggota familia Zingiberaceae. BioSMART, 3(2), 36-44.

Smith, R. M. (1990). Alpinia (Zingiberaceae): A proposed new infrageneric classification. Edinburgh Journal of Botany, 47, 1-75. doi: 10.1017/S0960428600003140.

Tang, Y. J., Liao, J. P., & Wu, Q. G. (2010). Comparative anatomy of leaves of Amomum (Zingiberaceae). Subtropical Plant Science, 39, 38-43. doi: 10.3969/j.issn.1009-7791.2010.03.012.

Willmer, C., & Fricker M. (2012). Stomata. Second Edition. London: Springer-Science+Business Media, B.V.

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