Effect of Honey, Aloe vera, and MEBO on Collagen Density in Healing Process of Second Degree Burns in Rats
Abstract
Abstrak
Berbagai penelitian ilmiah telah membuktikan bahwa kolagen merupakan biomaterial ideal dalam aktivitas penyembuhan luka. Senyawa bioaktif pada madu dan Aloe vera dapat membantu meningkatkan pembentukan kolagen. Penelitian ini dilakukan untuk membandingkan pengaruh aplikasi topikal madu, gel Aloe vera, dan Moist Exposed Burn Ointment (MEBO) terhadap kepadatan kolagen pada proses penyembuhan luka bakar derajat dua pada tikus. Luka bakar dibuat pada 28 ekor tikus dan dibagi menjadi empat kelompok yang dipilih secara acak serta dilakukan aplikasi topikal harian dengan NaCl, madu, gel Aloe vera, dan MEBO secara berurutan. Biopsi kulit dilakukan pada hari ke-7, kemudian dilakukan pembuatan sediaan histopatologi kulit dan dihitung kepadatan kolagennya. Uji One Way ANOVA menunjukkan kepadatan kolagen berbeda secara signifikan antar masing-masing kelompok (p= 0,009). Uji Post Hoc Bonferroni menunjukkan terdapat perbedaan yang signifikan antara kelompok NaCl dengan madu (p= 0,024) dan NaCl dengan MEBO (p= 0,024). Penelitian ini menunjukkan bahwa aplikasi madu dan MEBO secara topikal pada luka bakar derajat dua dapat meningkatkan pembentukan kolagen, sehingga dapat mempercepat proses penyembuhan luka. Madu, Aloe vera, dan MEBO dapat dijadikan sebagai terapi alternatif dalam penyembuhan luka bakar.
Abstract
Various scientific studies have proven that collagen is an ideal biomaterial in wound healing activities. The bioactive compounds in honey and Aloe vera can help increase collagen formation. This study was conducted to compare the effect of topical application of honey, Aloe vera gel, and MEBO (Moist Exposed Burn Ointment) on collagen density in the healing process of second-degree burns in rats. Burns were made on 28 rats which were further randomly divided into four groups to receive daily topical application of NaCl, Aloe vera, honey, and MEBO respectively. Skin biopsy was carried out on the seventh day, then histopathological preparation of the skin was made and collagen density was calculated. Result of One Way ANOVA test showed that collagen density differed significantly between groups (p= 0.009). The Post Hoc Bonferroni test resulted in significant difference between NaCl with honey group (p= 0.024) and NaCl with MEBO (p= 0.024). This study found that topical application of honey and MEBO to second-degree burns could increase collagen formation, thus accelerating wound healing process. Honey, Aloe vera, and MEBO can be used as alternative therapies for healing burns.
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Aftria, M. P. (2014). Honey as a topical treatment for diabetic foot ulcers. Medical Journal of Lampung University, 3(7), 81-87.
Akgun, S. G., Aydemir, S., Ozkan, N., Yuksel, M., & Sardas., S. (2017). Evaluation of the wound healing potential of Aloe vera-based extract of Nerium oleander. Northern Clinics of Instanbul, 4(3), 205-212. doi: 10.14744/nci.2017.94914.
Alam, A. J. N. (2019). Pengaruh pemberian gel Aloe vera terhadap kolagen luka. (Skripsi sarjana, Universitas Syiah Kuala, Banda Aceh, Indonesia). Retrieved from https://etd.unsyiah.ac.id/index.php?p=show_detail&id=59192
Ananda, I. P. B. (2018). Pengaruh pemberian madu dibandingkan lidah buaya (Aloe vera) terhadap kecepatan proses penyembuhan luka bakar pada tikus putih (Rattus norvegicus). (Skripsi sarjana, Universitas Wijaya Kusuma Surabaya, Surabaya, Indonesia). Retrieved from https://erepository.uwks.ac.id/3006/
Ashkani-Esfahani, S., Khoshneviszadeh, M., Noorafshan, A., Miri, R., Rafiee, S., Hemyari, K., … Faridi, E. (2019). The healing effect of Plantago major and Aloe vera mixture in excisional full thickness skin wounds: Stereological study. World Journal of Plastic Surgery, 8(1), 51-57. doi: 10.29252/wjps.8.1.51.
Badan Penelitian dan Pengembangan Kesehatan Kementerian Kesehatan RI. (2013). Riset kesehatan dasar tahun 2013. Jakarta: Badan Litbangkes, Depkes RI.
Biondo-Simões, M. L. P, Henning, J. L., Boen, B. R. O., Prado, J. L. D., Costa, L. R. D., Robes, R. R., & Ioshii, S. O. (2019). Comparative analysis of the effects of honey, copaiba oil-resin and a commercial product (fibrinolysin, deoxyribonuclease and chloramphenicol) on second intention healing, in rats. Revista Do Colégio Brasileiro de Cirurgiões, 46(5), e20192245. doi: 10.1590/0100-6991e-20192245.
Dewi, A. D. R., & Susanto, W. H. (2013). Pembuatan lempok pisang (Kajian jenis pisang dan konsentrasi madu). Jurnal Pangan dan Agroindustri, 1(1), 101-114.
El-Hadidy, M. R., El-Hadidy, A. R., Bhaa, A., Asker, S. A., & Mazroa, S. A. (2014). Role of epidermal stem cells in repair of partial-thickness burn injury after using moist exposed burn ointment (mebo®) histological and immunohistochemical study. Tissue and Cell, 46(2), 144-151. doi: 10.1016/j.tice.2014.01.002.
Farzadinia, P., Jofreh, N., Khatamsaz, S., Movahed, A., Akbarzadeh, S., Mohammadi, M., & Bargahi, A. (2016). Anti-inflammatory and wound healing activities of Aloe vera, honey and milk ointment on second-degree burns in rats. International Journal of Lower Extremity Wounds, 15(3), 241-247. doi: 10.1177/1534734616645031.
Felician, F. F., Yu, R-H., Li, M-Z., Li, C-J., Chen, H-Q., Jiang, Y., ... Xu, H.-M. (2019). The wound healing potential of collagen peptides derived from the jellyfish Rhopilema esculentum. Chinese Journal of Traumatology, 22(3), 12-20. doi: 10.1016/j.cjtee.2018.10.004.
Gould, L. J. (2016). Topical collagen-based biomaterials for chronic wounds: Rationale and clinical application. Advanced in Wound Care, 5(1), 19-31. doi: 10.1089/wound.2014.059.
Guo, S., & DiPietro, L. A. (2010). Factors affecting wound healing. Journal of Dental Research, 89(3), 219-229. doi: 10.1177/0022034509359125.
Hashemi, S. A., Madani, S. A., & Abediankenari, S. (2015). The review on properties of Aloe vera in healing of cutaneous wounds. BioMed Research International, 2015, 1-6. doi: 10.1155/2015/714216.
Hekmatpou, D., Mehrab, F., Rahzani, K., & Aminiyan, A. (2019). The effect of Aloe vera clinical trials on prevention and healing of skin wound: A systematic review. Iranian Journal of Medical Sciences, 44(1), 1-9.
Ibrahim, N. ‘I., Wong, S. K., Mohamed, I. N., Mohamed, N., Chin, K. Y., Ima-Nirwana, S., & Shuid, A. N. (2018). Wound healing properties of selected natural products. International Journal of Environmental Research and Public Health, 15(11), 2360. doi: 10.3390/ijerph15112360.
Indrawan., Dachlan, I., & Purnomosari, D. (2016). Perbandingan pengaruh aplikasi Aloe vera, madu, saliva dan putih telur terhadap ekspresi interleukin-6 pada proses penyembuhan luka insisi kulit tikus. (Spesialisasi, Tesis, Universitas Gadjah Mada, Yogyakarta, Indonesia). Retrieved from http://etd.repository.ugm.ac.id/home/detail_pencarian/105764
Isrofah., Sagiran., & Afandi, M. (2015). Efektifitas salep ekstrak daun binahong (Anredera cordifolia (Ten) Steenis) terhadap proses penyembuhan luka bakar derajat 2 termal pada tikus putih (Rattus novergicus). Muhammadiyah Journal of Nursing, 2(1), 28-36.
Jull, A. B., Cullum, N., Dumville, J. C., Westby, M. J., Deshpande, S., & Walker, N. (2015). Honey as a topical treatment for wounds (Review) summary of findings for the main comparison. Cochrane Library, (3), CD005083. doi: 10.1002/14651858.CD005083.pub4.www.cochranelibrary.com.
Kim, Y. A., Gaidin, S. G., & Tarahovsky, Y. S. (2018). The influence of simple phenols on collagen type I fibrillogenesis in vitro. Biophysics (Russian Federation), 63(2), 162-168. doi: 10.1134/S0006350918020148.
Li, W., Ma, Y., Yang, Q., Pan, Y., & Meng, Q. (2017). Moist exposed burn ointment for treating pressure ulcers. Medicine (United States), 96(29), 1-5. doi: 10.1097/MD.0000000000007582.
Linangkung, A., Dachlan, I., & Purnomosari, D. (2017). Perbandingan pengaruh aplikasi Aloe vera, madu, saliva, dan putih telur terhadap ekspresi interleukin 10 pada proses penyembuhan luka insisi kulit tikus. (Spesialisasi, Tesis, Universitas Gadjah Mada, Yogyakarta, Indonesia). Retrieved from http://etd.repository.ugm.ac.id/home/detail_pencarian/109409
Marks, D. B., Marks, A. D., & Smith, C. M. (2014). Biokimia Kedokteran Dasar. Jakarta: Penerbit Buku Kedokteran EGC.
Martinotti, S., & Ranzato, E. (2018). Honey, wound repair and regenerative medicine. Journal of Functional Biomaterials, 9(2), 34. doi: 10.3390/jfb9020034.
Masfufatun, M., Tania, P. O. A., Raharjo, L. H., & Baktir, A. (2018). Kadar IL-6 dan IL-10 serum pada Tahapan inflamasi di Rattus norvegicus yang terinfeksi Candida albicans. Jurnal Kedokteran Brawijaya, 30(1), 19. doi: 10.21776/ub.jkb.2018.030.01.4.
Nimma, V. L., Talla, H. V., Bairi, J. K., Gopaldas, M., Bathula, H., & Vangdoth, S. (2017). Holistic healing through herbs: Effectiveness of Aloe vera on post extraction socket healing. Journal of Clinical and Diagnostic Research, 11(3), ZC83-ZC86. doi: 10.7860/JCDR/2017/21331.9627.
Novitasari, R. K., Setiyajati, A., & Haris, R. A. (2015). Perbedaan efektivitas penggunaan lidah buaya dan madu terhadap penyembuhan luka bakar grade II pada tikus wistar jantan. Jurnal Ilmu Keperawatan Indonesia (JIKI), 8(2).
Oryan, A., Mohammadalipour, A., Moshiri, A., & Tabandeh, M. R. (2016). Topical application of Aloe vera accelerated wound healing, modeling, and remodeling. Annals of Plastic Surgery, 77(1), 37-46. doi: 10.1097/SAP.0000000000000239.
Oryan, A., Alemzadeh, E., & Moshiri, A. (2016). Biological properties and therapeutic activities of honey in wound healing: A narrative review and meta-analysis. Journal of Tissue Viability, 25(2), 98-118. doi: 10.1016/j.jtv.2015.12.002.
Palumpun, E. F., Wiraguna, A. A. G. P., & Pangkahila, W. (2017). Pemberian ekstrak daun sirih (Piper betle) secara topikal meningkatkan ketebalan epidermis, jumlah fibroblas, dan jumlah kolagen dalam proses penyembuhan luka pada tikus jantan galur Wistar (Rattus norvegicus). Jurnal E-Biomedik, 5(1). doi: 10.35790/ebm.5.1.2017.15037.
Pencle, F. J., Mowery, M. L., & Zulfiqar, H. (2019). First degree burn. (2020, December 3). Retrieved from https://www.ncbi.nlm.nih.gov/books/NBK442021/
Prasetyo, A. T., & Heridadi, E. (2013). The application of moist exposed burn ointment (MEBO) and binahong leaves in treating partial thickness burn : A case report. Jurnal Plastik Rekonstruksi, 2(4), 142-146. doi: 10.14228/jpr.v2i4.178.
Priscilla, L. (2017). Perbandingan efektivitas aplikasi topikal lidah buaya (Aloe vera), madu (Apis mellifera), dan kombinasi keduanya terhadap penyembuhan luka sayat pada kulit mencit (Mus musculus). (Skripsi sarjana, Universitas Hasanuddin, Makassar, Indonesia). Retrieved from https://core.ac.uk/download/pdf/89562576.pdf
Purnomo, S. E. C., Dwiningsih, S. U., & Lestari, K. P. (2014). Efektifitas penyembuhan luka menggunakan NaCl 0,9% dan hydrogel pada ulkus diabetes di RSU kota Semarang. Paper presented at Prosiding Konferensi Nasional II PPNI, Jawa Tengah, Indonesia. Retrieved from https://jurnal.unimus.ac.id/index.php/psn12012010/article/view/1136
Rosanto, Y. B., Handajani, J., & Susilowati, H. (2012). Efek pemberian gel getah batang tanaman pisang secara topikal terhadap kepadatan serabut kolagen pada proses penyembuhan luka pasca ekstraksi gigi marmut. Dentika Dental Journal, 17(1), 34-39.
Schencke, C., Vasconcellos, A., Sandoval, C., Torres, P., Acevedo, F., & del Sol, M. (2016). Morphometric evaluation of wound healing in burns treated with Ulmo (Eucryphia cordifolia) honey alone and supplemented with ascorbic acid in guinea pig (Cavia porcellus). Burns & Trauma, 4, 1-9. doi: 10.1186/s41038-016-0050-z.
Syahdrajat, T. (2015). Panduan menulis tugas akhir kedokteran & kesehatan. Jakarta: Kencana.
Takzaree, N., Hassanzadeh, G., Rouini, M. R., Manayi, A., Hadjiakhondi, A., & Majidi Zolbin, M. (2017). Evaluation of the effects of local application of thyme honey in open cutaneous wound healing. Iranian Journal of Public Health, 46(4), 545-551.
Tang, Q. L., Han, S. S., Feng, J., Di, J. Q., Qin, W. X., Fu, J., & Jiang, Q. Y. (2014). Moist exposed burn ointment promotes cutaneous excisional wound healing in rats involving VEGF and bFGF. Molecular Medicine Reports, 9(4), 1277-1282. doi: 10.3892/mmr.2014.1921.
Teplicki, E., Ma, Q., Castillo, D. E., Zarei, M., Hustad, A. P., Chen, J., & Li, J. (2018). The effects of Aloe vera on wound healing in cell proliferation, migration, and viability. Wounds, 30(9), 263-268.
Tomo, W. K., Dachlan, I., & Purnomosari, D. (2015). Perbandingan pengaruh aplikasi Aloe vera, madu, saliva dan putih telur terhadap pembentukan kolagen pada proses penyembuhan luka insisi kulit tikus dengan pewarnaan mallory. (Spesialisasi, Tesis, Universitas Gadjah Mada, Yogyakarta, Indonesia). Retrieved from http://etd.repository.ugm.ac.id/penelitian/detail/88709
World Health Organization. (2018). Burns. (2018, March 6). Retrieved from https://www.who.int/news-room/fact-sheets/detail/burns
Yuza, F., Wahyudi, I. A., & Larnani, S. (2014). Efek pemberian ekstrak lidah buaya (Aloe barbadensis Miller) pada soket gigi terhadap kepadatan serabut kolagen pasca ekstraksi gigi marmut (Cavia porcellus). Majalah Kedokteran Gigi Indonesia, 21(2), 127. doi: 10.22146/majkedgiind.8743.
Zhou, S., Salisbury, J., Preedy, V. R., & Emery, P. W. (2013). Increased collagen synthesis rate during wound healing in muscle. PLoS ONE, 8(3), 8-11. doi: 10.1371/journal.pone.0058324.
DOI: https://doi.org/10.15408/kauniyah.v14i1.16734 Abstract - 0 PDF - 0
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