UJI AKTIVITAS ANTIOKSIDAN EKSTRAK BAWANG HITAM (BLACK GARLIC) DENGAN VARIASI LAMA PEMANASAN
Abstract
Abstrak
Senyawa radikal dalam tubuh sangat reaktif terhadap sel-sel, sehingga dapat menyebabkan berbagai penyakit. Senyawa yang mampu menangkal radikal bebas disebut antioksidan. Bawang hitam diketahui mengandung senyawa aktif fenolik dan turunannya sehingga dapat digunakan sebagai antioksidan. Penelitian tentang penggunaan bawang hitam sebagai antioksidan ini bertujuan untuk mengetahui perbedaan waktu pemanasan (15, 25, dan 35 hari) terhadap aktivitas antioksidan pada ekstrak bawang hitam. Bawang hitam diperoleh dengan cara memanaskan bawang putih selama 15, 25, dan 35 hari. Bawang hitam selanjutnya diekstraksi dengan pelarut metanol dengan metode maserasi. Filtrat hasil ekstraksi dipekatkan dengan rotary evaporator untuk mendapatkan ekstrak bawang hitam. Ekstrak bawang hitam diuji fitokimia untuk mengetahui kandungan senyawa aktif. Aktivitas antioksidan ekstrak bawang hitam ditentukan dengan metode penangkalan radikal 2,2-diphenyl-1-picrylhydrazyl (DPPH). Hasilnya menunjukkan ekstrak bawang hitam memiliki aktivitas antioksidan yang sangat kuat dengan nilai IC50 masing-masing 15 hari yaitu 2,41 µg/mL; 25 hari yaitu 2,93 µg/mL; 35 hari yaitu 2,27 µg/mL. Nilai IC50 <10 μg/mL menunjukkan bahwa ekstrak bawang hitam dengan waktu pemanasan 15, 25, dan 35 hari memiliki potensi antioksidan yang sangat kuat. Waktu pemanasan yang paling optimum dalam penangkalan radikal bebas adalah ekstrak bawang hitam dalam waktu pemanasan 35 hari, karena banyak terdapat kandungan senyawa flavonoid, tanin, sterol, dan saponin.
Abstract
Radical compounds in the body are very reactive to cells that can cause various diseases. Compounds that are able to ward off free radicals are called antioxidants. Black garlic are known to contain phenolic active compounds and their derivatives so that they can be used as antioxidants. Research on the use of black garlic as an antioxidant aims to determine the difference in heating time (15, 25, and 35 days) on the antioxidant activity of black garlic extract. Black garlic are obtained by heating the garlic for 15, 25, and 35 days. The black garlic is then extracted with methanol solvent by maceration method. The extracted filtrate was concentrated with a rotary evaporator to obtain the black garlic extract. Black garlic extract was tested for phytochemistry to determine the content of active compounds. The antioxidant activity of black garlic extract was determined by the radical deterrence method 2,2-diphenyl-1-picrylhydrazyl (DPPH). The results showed that the onion extract had a very strong antioxidant activity with an IC50 value of 15 days each of 2.41 µg/mL; 25 days which is 2.93 μg/mL; 35 days which is 2.27 µg/mL. IC50 values <10 μg/mL indicate that the black garlic extract with a heating time of 15, 25, and 35 days has a very strong antioxidant potential. The most optimum heating time in deterring free radicals is black garlic extract within 35 days of heating because there are many compounds containing flavonoids, tannins, sterols, and saponins.
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Amagase, H. (2006). Clarifying the real bioactive constituents of garlic. The Journal of Nutrition, 136(3), 716S-725S. doi: 10.1093/jn/136.3.716S.
Angeles, T. M. M., Jesus, P. A., Rafael, M. R., & Tania, M. A. (2016). Evolution of some physicochemical and antioxidant properties of black garlic whole bulbs and peeled cloves. Food Chemistry, 199, 135-139. doi: 10.1016/j.foodchem.2015.11.128.
Bae, S. E., Cho, S. Y., Won, Y. D., Lee, S. H., & Park, H. J. (2014). Changes in S-allyl cysteine contents and physicochemical properties of black garlic during heat treatment. LWT-Food Science and Technology, 55(1), 397-402. doi: 10.1016/j.lwt.2013.05.006.
Chen, Y.-C., Kao, T.-H., Tseng, C.-Y., Chang, W.-T., & Hsu, C.-L. (2014). Methanolic extract of black garlic ameliorates diet-induced obesity via regulating adipogenesis, adipokine biosynthesis, and lipolysis. Journal of Functional Foods, 9, 98-108. doi: 10.1016/j.jff.2014.04.014.
Choi, J.-G., Mun, S.-H., Chahar, H. S., Bharaj, P., Kang, O.-H., Kim, S.-G., … Kwon, D.-Y. (2014). Methyl gallate from Galla rhois successfully controls clinical isolates of Salmonella infection in both in vitro and in vivo systems. PLOS ONE, 9(7), e102697. doi: 10.1371/journal.pone.0102697.
Ghiselli, A., Nardini, M., Baldi, A., & Scaccini, C. (1998). Antioxidant activity of different phenolic fractions separated from an Italian red wine. Journal of Agricultural and Food Chemistry, 46(2), 361-367. doi: 10.1021/jf970486b.
Halliwell, B., & Gutteridge, J. M. C. (2015). Free radicals in biology and medicine (fifth edition). United Kingdom: Oxford University Press.
Hernawan, U. E., & Setyawan, A. D. (2003). Review: Senyawa organosulfur bawang putih (Allium sativum L.) dan aktivitas biologinya. Biofarmasi, 1(2), 65-76.
Irianti, T., Puspitasari, A., Machwiyyah, L., & Rabbani, H. R. (2016). The activity of radical scavenging of 2,2-diphenyl-1-pycrilhydrazil (DPPH) by ethanolic extracts of mengkudu leaves (Morinda citrifolia L.), brotowali stem (Tinospora crispa L.), its water fraction and its hydrolized fraction. Majalah Obat Tradisional, 20(3), 140-148. doi: 10.22146/tradmedj.8850.
Jang, J.-E., Kim, S.-W., Kim, S.-Y., Kim, J.-M., Park, M.-H., Yoon, J.-H., … Yoon, J.-S. (2013). Religiosity, depression, and quality of life in Korean patients with breast cancer: A 1-year prospective longitudinal study. Psycho-Oncology, 22(4), 922-929. doi: 10.1002/pon.3083.
Kementerian Kesehatan RI. (2015). Situasi penyakit kanker. Jakarta: Pusat Data Dan Informasi Keementerian Kesehatan Republik Indonesia.
Kimura, S., Tung, Y.-C., Pan, M.-H., Su, N.-W., Lai, Y.-J., & Cheng, K.-C. (2017). Black garlic: A critical review of its production, bioactivity, and application. Journal of Food and Drug Analysis, 25(1), 62-70. doi: 10.1016/j.jfda.2016.11.003.
Liang, N., & Kitts, D. D. (2014). Antioxidant property of coffee components: Assessment of methods that define mechanisms of action. Molecules, 19(11), 19180-19208. doi: 10.3390/molecules191119180.
Lu, X., Li, N., Qiao, X., Qiu, Z., & Liu, P. (2017). Composition analysis and antioxidant properties of black garlic extract. Journal of Food and Drug Analysis, 25(2), 340-349. doi: 10.1016/j.jfda.2016.05.011.
Marliana, S. D., Suryanti, V., & Suyono. (2005). Skrining Fitokimia dan analisis kromatografi lapis tipis komponen kimia buah labu siam (Sechium edule Jacq. Swartz.) dalam ekstrak etanol. Biofarmasi, 3(1), 26-31.
Mukhriani, M., Nonci, F. Y., & Mumang, M. (2017). Penetapan kadar tanin total ekstrak biji jintan hitam (Nigella sativa) secara spektrofotometri uv-vis. Jurnal Farmasi UIN Alauddin Makassar, 2(4), 154-158.
Nijveldt, R. J., van Nood, E., van Hoorn, D. E., Boelens, P. G., van Norren, K., & van Leeuwen, P. A. (2001). Flavonoids: A review of probable mechanisms of action and potential applications. The American Journal of Clinical Nutrition, 74(4), 418-425. doi: 10.1093/ajcn/74.4.418.
Prakash, A., Rigelhoft, F., & Miller, E. (2001). Antioxidant activity. Medallion Laboratories-Analytical Progress, 19(2), 1-4.
Prasonto, D., Riyanti, E., & Gartika, M. (2017). Uji aktivitas antioksidan ekstrak bawang putih (Allium sativum). Odonto: Dental Journal, 4(2), 122-128. doi: 10.30659/odj.4.2.122-128.
Redha, A. (2010). Flavonoid: Struktur, sifat antioksidatif dan peranannya dalam sistem biologis. Jurnal Belian, 9(2), 196-202.
Nb, A. (2019, October 10). Anton nb: Antioksidan kunci agar selalu awet muda dan hidup sehat lebih lama. Anton Nb. https://www.anton-nb.com/2019/10/antioksidan-kunci-agar-selalu-awet-muda.html
Ryu, J. H., & Kang, D. (2017). Physicochemical properties, biological activity, health benefits, and general limitations of aged black garlic: A review. Molecules, 22(6), 919-932. doi: 10.3390/molecules22060919.
Sasaki, J., Lu, C., Machiya, E., Tanahashi, M., & Hamada, K. (2007). Processed black garlic (Allium sativum) extracts enhance anti-tumor potency against mouse tumors. Medical and Aromatic Journal of Plant Science and Biotechnology, 1, 278-281.
Sengin, E. L. (1992). Perbanyakan mikro pada tanaman bawang putih (Allium sativum L.) (Disertasi doktoral, Institute Pertanian Bogor, Bogor, Indonesia). Retrieved from http://repository.ipb.ac.id/handle/123456789/23136
Song, K., & Milner, J. A. (2001). The influence of heating on the anticancer properties of garlic. The Journal of Nutrition, 131(3), 1054S-1057S. doi: 10.1093/jn/131.3.1054S.
Teow, C. C., Truong, V.-D., McFeeters, R. F., Thompson, R. L., Pecota, K. V., & Yencho, G. C. (2007). Antioxidant activities, phenolic and β-carotene contents of sweet potato genotypes with varying flesh colours. Food Chemistry, 103(3), 829-838. doi: 10.1016/j.foodchem.2006.09.033.
Ulfa, E. U., & Hidayat, M. A. (2007). Uji aktivitas antioksidan ekstrak buah naga (Hylocereus undatus (Haw.) Britt. & Rose). Jurnal Ilmu Dasar, 8(1), 83-90.
Velázquez, E., Tournier, H. A., Mordujovich de Buschiazzo, P., Saavedra, G., & Schinella, G. R. (2003). Antioxidant activity of Paraguayan plant extracts. Fitoterapia, 74(1-2), 91-97. doi: 10.1016/s0367-326x(02)00293-9.
Wang, D., Feng, Y., Liu, J., Yan, J., Wang, M., Sasaki, J., & Lu, C. (2010). Black garlic (Allium sativum) extracts enhance the immune system. Medical and Aromatic Journal of Plant Science and Biotechnology, 4(1), 37-40.
Zhang, X., Li, N., Lu, X., Liu, P., & Qiao, X. (2016). Effects of temperature on the quality of black garlic. Journal of the Science of Food and Agriculture, 96(7), 2366-2372. doi: 10.1002/jsfa.7351.
DOI: https://doi.org/10.15408/kauniyah.v13i1.12114 Abstract - 0 PDF - 0
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