KARAKTERISASI DAN IDENTIFIKASI BAKTERI RIZOSFER TANAMAN SAWIT JAMBI

Khairani Khairani, Fitratul Aini, Hesti Riany

Abstract


Abstrak

Bakteri rizosfer adalah bakteri yang terdapat pada daerah perakaran tanaman yang diketahui memiliki keanekaragaman tinggi. Bakteri rizosfer memiliki berbagai peran seperti menyediakan nutrisi bagi tanaman, melindungi tanaman dari infeksi bakteri patogen, menghasilkan hormon pertumbuhan seperti indol acetic acid, pelarut fosfat, pengikat nitrogen, dan lain-lain. Dengan berbagai kemampuan dan peran tersebut, maka perlu eksplorasi bakteri rizosfer tanaman sawit khususnya di daerah perkebunan agar diketahui kelompok bakteri rizosfer, sehingga dapat dimanfaatkan secara optimal. Penelitian ini bertujuan untuk mengetahui kelompok dan keragaman bakteri rizosfer pada tanaman sawit yang berumur 8, 11, dan 14 tahun. Penelitian dilakukan dengan pendekatan konvensional dengan karakterisasi morfologi dan uji biokimia. Dari umur-umur tersebut, didapatkan 18 isolat yang terdiri dari 11 genus, yaitu Bacillus, Azospirillum, Actinobacillus, Xanthobacter, Enterococcus, Paenibacillus, Klebsiella, Pseudomonas, Arthrobacter, Micrococcus, dan Streptococcus. Bakteri rizosfer tanaman sawit memiliki keragaman genus yang sama pada umur 8, 11, dan 14 tahun. Genus bakteri yang didapatkan dapat dimanfaatkan sebagai PGPR (Plant Promoting Rhizobacteria).

Abstract

Rhizosphere bacteria are bacteria found in root areas. Rhizosphere bacteria have various roles such as providing nutrients for plants, protecting plants from infection pathogenic bacteria, producing growth hormones such as indol acetic acid, phosphate solvents, nitrogen binders, and others. By having these various abilities and roles, it is necessary to explore the bacteria of oil palm plants, especially in the plantation areas so that the group of rhizosphere bacteria can be identified and then utilized optimally. This study aims to determine the group and diversity of rhizosphere bacteria in oil palm plants that are 8, 11, and 14 years old. The research was conducted conventionally by observing morphological and biochemical characterization. At those ages, 18 isolates were consisting of 11 genera, namely Bacillus, Azospirillum, Actinobacillus, Xanthobacter, Enterococcus, Paenibacillus, Klebsiella, Pseudomonas, Arthrobacter, Micrococcus, and Streptococcus through conventional methods. Rhizosphere bacteria in oil palm plants have the same genus diversity at the ages of 8, 11, and 14 years. The bacterial genus obtained can be used as PGPR (Plant Promoting Rhizobacteria).


Keywords


Bacillus; Klebsiella pneumoniae; Paenibacillus; Rhizosphere; Sawit; Palm

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References


Brenner, D. J., Krieg, N. R., & Staley, J. T. (2005). Bergey’s manual of systemic bacteriology second edition, volume three: the Firmicutes. USA: Department of Microbiology and Moleculer Genetics, Michigan State University.

Bhardwaj, G., Shah, R., Joshi, B., & Patel, P. (2017). Klebsiella pneumoniae VRE36 as a PGPR isolated from Saccharum officinarum cultivar Co99004. Journal of Applied Biology and Biotechnology, 5(01), 047-052. doi: 10.7324/jabb.2017.50108

De Vos, P., Garrity, G. M., Jones, D., Krieg, N. R., Ludwig, W., Rainey, F. A., Schleifer, K. H., & Whitman, W. B. (2009). Bergey’s manual of systemic bacteriology second edition, volume three: the Firmicutes. USA: Department of Microbiology, University of Georgia Athens.

Dewi, N. (2015). Uji antagonis bakteri rizosfer pisang terhadap cendawan Rhyzoctonia solani (Skripsi). Fakultas Sains dan Teknologi, Universitas Alauddin Makassar, Makassar.

Flores-Núñez, V. M., Amora-Lazcano, E., Rodríguez-Dorantes, A., Cruz-Maya, J. A., & Jan-Roblero, J. (2018). Comparison of plant growth-promoting rhizobacteria in a pine forest soil and an agricultural soil. Soil Research, 56(4), 346. doi: 10.1071/sr17227

Holt, J. G., Krieg, N. R., Sneath, P. H. A., Staley, J. T., Williams, S. T. (2000). Bergey’s manual of determinative bacteriology ninth edition. Maryland: Lippicott Williams & Wilkins, a wolters Kluwer company.

Gao, P., Tian, H., Wang, Y., Li, Y., Li, Y., Xie, J., … Ma, T. (2016). Spatial isolation and environmental factors drive distinct bacterial and archaeal communities in different types of petroleum reservoirs in China. Scientific Reports, 6(20174), 1-12. doi: 10.1038/srep20174

Grady, E. N., MacDonald, J., Liu, L., Richman, A., & Yuan, Z. C. (2016). Current knowledge and perspectives of Paenibacillus: a review. Microbial Cell Factories, 15(1), 1-18. doi: 10.1186/s12934-016-0603-7

Guo, Y., Huang, E., Yuan, C., Zhang, L., & Yousef, A. E. (2012). Isolation of a Paenibacillus sp. Strain and structural elucidation of its broad-spectrum lipopeptide antibiotic. Applied and Environmental Microbiology, 78(9), 3156-3165. doi: 10.1128/AEM.07782-11.

Heulin, T., Achouak, W., Berge, O., Normand, P., & Guinebretière, M. H. (2015). Paenibacillus graminis sp. nov. and Paenibacillus odorifer sp. nov., isolated from plant roots, soil and food. International Journal of Systematic and Evolutionary Microbiology, 52(2), 607-616. doi: 10.1099/00207713-52-2-607.

Khan, Z., Hussain, S. Z., Rehman, A., Zulfiqar, S., & Shakoori, A. R. (2015). Evaluation of cadmium resistant bacterium, Klebsiella Pneumoniae, isolated from industrial wastewater for its potential use to bioremediate environmental cadmium. Pakistan Journal of Zoology, 47(6), 1533-1543.

Kielak, A., Pijl, A. S., Van Veen, J. A., & Kowalchuk, G. A. (2009). Phylogenetic diversity of Acidobacteria in a former agricultural soil. International Society for Microbial Ecology, Journal, 3(3), 378-382. doi: 10.1038/ismej.2008.113

Nagamani, A., Soligalla, R., & Lowry, M. (2009). Isolation and characterization of phenol degrading Xanthobacter flavus. African Journal of Biotechnology, 8(20): 5449-5453. doi: 10.4314/ajb.v8i20.65988

Nofu, K., Khotimah, S., & Lovadi, I. (2014). Isolasi dan karakteristik bakteri pendegradasi selulosa pada ampas tebu kuning (bagasse). Protobiont, 3(1), 25-33.

Paczosa, M. K., & Mecsas J. (2016). Klebsiella pneumoniae: going on the offense with a strong defense. Microbiology and Molecular Biology Reviews, 80(3), 629-661.

Priest, F. G. (2015). Genus Paenibacillus. In P. De Vos, G. M. Garrity, D. Jones, N. R. Krieg, W. Ludwig, F. A. Rauney, K. Schleifer, & W. B. Whitman (Eds.), Bergeys’s manual of systematic bacteriology (pp. 269-295). New York, USA: Springer.

Puspita, F., Ali, M., & Pratama, R. (2017). Isolasi dan karakterisasi morfologi dan fisiologi bakteri Bacillus sp. endofitik dari tanaman kelapa sawit (Elaeis guineensis Jacq.). Jurnal Agroteknologi Tropika, 6(2), 44-49.

Rashmi, Y. C., Reshmi, R., Poornima, R., & Kumar, S. (2017). Isolation and Characterization of Microorganisms from Agriculture Soil of Magnifera indica Orchard. International Journal of Current Microbiology and Applied Science, 6(6), 2707-2713.

Rizqi, D. Y. (2018). Isolasi dan identifikasi bakteri pendegradasi hidrokarbon dari oli bekas (Skripsi). Jurusan Pendidikan Biologi, Fakultas Tarbiyah dan Pendidikan Universitas Negeri Mataram, Mataram.

Roa, J., Cadavid, M., Muñoz, F., Chica, H. A., & Ángel, C. A. (2016, December 5-8). Isolation and preliminary biochemical characterization of nitrogen-fixing bacteria belonging to three genera obtained from sugarcane in Colombia. Paper presented at Proceedings of the International Society of Sugar Cane Technologists International Society of Sugar Cane Technologist (ISSCT) XXIX Congress, At Chiang Mai International Exhibition and Convention Centre, Thailand, Retrieved from https://www.researchgate.net/publication/313847261

Sachdev, D. P., Chaudhari, H. G., Kasture, V. M., Dhavale, D. D., & Chopade, B. A. (2009). Isolation and characterization of indole acetic acid (IAA) producing Klebsiella pneumoniae strains from rhizosphere of wheat (Triticum aestivum) and their effect on plant growth. Indian Journal of Experimental Biology, 47(12), 993-1000.

Santoso, K., Rahmawati., & Rafdinal. (2019). Eksplorasi bakteri penambat nitrogen dari tanah hutan mangrove Sungai Peniti, Kabupaten Mempawah. Protobiont, 8(1), 52-58.

Saraswati, R. (1999). Teknologi pupuk mikrob multiguna menunjang keberlanjutan sistèm produksi kedelai. Jurnal Mikrobiologi Indonesia, 4(1), 1-9.

Schneider, D., Engelhaupt, M., Allen, K., Kurniawan, S., Krashevska, V., Heinemann, M., & Daniel, R. (2015). Impact of lowland rainforest transformation on diversity and composition of soil prokaryotic communities in Sumatra (Indonesia). Frontiers in Microbiology, 6(1339)1-12.

Sathya, A., Vijayabharathi, R., & Gopalakrishnan, S. (2017). Plant growth-promoting Actinobacteria: a new strategy for enhancing sustainable production and protection of grain legumes. 3 Biotech Springer, 7(2), 1-10. doi: 10.1007/s13205-017-0736-3

Sitepu, I. R., Hashidoko, Y., & Turjaman, M. (2010). Aplikasi Rhizobakteri penghasil fitohormon untuk meningkatkan pertumbuhan bibit Aquilaria sp. di persemaian Info Hutan, 7(2), 107-116.

Suriawiria, U. (2005). Mikrobiologi dasar. Jakarta: Papas Sinar Sinanti.

Susanti, Y. (2014). Eksplorasi agen antagonis disekitar perakaran tanaman kelapa sawit (Elaeis guineensis Jacq.) di Kabupaten Rokan Hulu. Jurnal Sungkai, 2(1), 37-42.

Susilawati., Budhisurya, E., Anggono, R. C. W., & Simanjuntak, B. H. (2016). Analisis kesuburan tanah dengan indikator mikroorganisme tanah pada berbagai sistem penggunaan lahan di Plateau Dieng. Jurnal Ilmu Pertanian (Agric), 25(1), 64. doi: 10.24246/agric.2013.v25.i1.p64-72

Tsuji, A., Takahashi, K., Kaneko, Y., Goto, S., & Ogawa, M. (2013). The effects of temperature and ph on the growth of eight enteric and nine glucose non-fermenting species of gram-negative rods. Microbiology and Immunology, 26(1), 15-24. doi: 10.1111/j.1348-0421.1982.tb00149

Verma, M., Mishra, J., & Arora, N. K. (2018). Plant promoting rhizobacteria: diversity and applications. In R. C. Sobti, N. K Arora, & R. Kothari (Eds.), Environmental biotechnology: for sustainable future (pp. 129-173). Singapore: Springer Nature Singapore.

Wafula, E. N. (2013). Analyses of soil bacteria in Ngere Tea Catchment Area of Murang’a County, Kenya (Master’s thesis). Jomo Kenyatta University of Agriculture and Technology, Kenya.

Weigel, J. (2006). The genus Xanthobacter. In S. Falkow, E, Rosenberg, K. H. Schleifer, & E. Stackrebrandt (Eds.), The prokaryotes: a handbook on the biology of bacteria (pp. 290-314). New York, USA: Springer Science & Business Media.

Xie, J. B., Du, Z., Bai, L., Tian, C., Zhang, Y., Xie, J. Y., Li, J. (2014). Comparative genomic analysis of n 2 -fixing and non-n 2 -fixing Paenibacillus spp.: organization, evolution and expression of the nitrogen fixation genes. PLoS Genetics, 10(3), 1-17 doi: 10.1371/journal.pgen.1004231

Xie, J., Shi, H., Du, Z., Wang, T., Liu, X., & Chen, S. (2016). Comparative genomic and functional analysis reveal conservation of plant growth promoting traits in Paenibacillus polymyxa and its closely related species. Scientific Reports, 6(21329), 1-12. doi: 10.1038/srep21329

Yang, Y., Wang, N., Guo, X., Zhang, Y., & Ye, B. (2017). Comparative analysis of bacterial community structure in the rhizosphere of maize by highthroughput pyrosequencing. PLoS ONE, 12(5), 1-11. doi: 10.1371/journal.pone.0178425.




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