The Impact of High Dose Ozone Therapy on Hematological and Biochemical Profiles : An Observational Study

Authors

DOI:

https://doi.org/10.15408/avicenna.v5i1.34859

Keywords:

High Dose Ozone Therapy, hematological alterations, biochemical alterations, oxidative stress markers

Abstract

Background: Ozone therapy, namely High Dose Ozone Therapy (HDO), has become a versatile medical treatment in several clinical settings. Although it is becoming more well acknowledged, there is still a lack of extensive evidence about the impact of this on hematological and biochemical indicators. This study seeks to address this void by examining the consequences of this phenomenon in a clinical environment.

Method: An observational study was done, which followed a structured approach, comprising a total of 100 patients who were selected from the SMC Clinic. Hematological and biochemical indices were evaluated before and after ozone therapy sessions to analyze blood parameters. Two blood samples were obtained at two specific time points: prior to the commencement of the ozone therapy (T0) and after the therapy was finished (T1).

Results: Substantial alterations were noted in both hematological and biochemical parameters after the therapy. The hematological changes observed in this study showed a substantial rise in the red blood cell count (p=0.009), hemoglobin levels (p=0.004), and hematocrit (p=0.039), along with a decrease in erythrocyte sedimentation rate (ESR) (p=0.020). Notable biochemical changes were observed, including significant modifications in total plasma protein (TPP) (p=0.008) and lactate levels (p=0.001). Furthermore, there was a noteworthy decrease (p<0.05) in the levels of malondialdehyde (MDA), which is a marker for oxidative stress.

Conclusion: HDO exerts a regulatory influence on crucial hematological, biochemical, and oxidative stress factors. These findings indicate that HDO may have therapeutic benefits for treating chronic disorders and cancer. It can improve blood parameters and reduce oxidative stress. The decrease in markers of oxidative stress, such as MDA, suggests a potential reduction in the likelihood of problems associated with oxidative damage in chronic diseases. Nevertheless, additional research is required to validate these findings, investigate their wider therapeutic implications, and comprehend the long-term consequences and safety of HDO therapy.

Author Biographies

  • Reza Aditya Digambiro, Department of Anatomical Pathology, Faculty of Medicine- Trisakti University
    Dr. Reza Aditya Digambiro, M.Kes, M.Ked(PA), Sp.PA is currently affiliated with the Faculty of Medicine at Universitas Trisakti, Indonesia. With a background in Anatomical Pathology, Dr. Digambiro is actively involved in research and academic pursuits in the medical field. His comprehensive knowledge and expertise are reflected in various research projects, focusing primarily on the advancements in medical science and healthcare. For more information on his works and contributions, please visit https://fk.trisakti.ac.id/ or refer to his ORCID profile at https://orcid.org/0000-0003-4692-7648.
  • Edy Parwanto, Department of Biology, Faculty of Medicine- Trisakti University

    Dr. Edy Parwanto is a distinguished member of the Department of Biology at the Faculty of Medicine, Universitas Trisakti, Indonesia. With a strong background in the field of Biology, Dr. Parwanto has been instrumental in fostering research and academic excellence in his department. His commitment to the advancement of science and education is evidenced by his active participation in various research initiatives. His scholarly contributions and research endeavors can be further explored through his ORCID profile: https://orcid.org/0000-0002-0797-6925. He can be reached for academic and professional correspondence at edyparwanto@trisakti.ac.id.

  • Indah Widya Lestari, Department of Anatomical Pathology – Faculty of Medicine – Universitas Trisakti

    Dr. Indah Widya Lestari, Sp.PA serves as a reputable member of the Department of Anatomical Pathology at the Faculty of Medicine, Universitas Trisakti, Indonesia. Engaged in significant research and academic activities, Dr. Lestari is known for her expertise and substantial contributions to the field of Anatomical Pathology. Her research portfolio and academic contributions can be further explored through her ORCID profile: https://orcid.org/0009-0009-5539-7598. She can be contacted for scholarly correspondence at indahwidya@trisakti.ac.id.

     

  • Julian Chendrasari, Department of Anatomical Pathology – Faculty of Medicine – Universitas Trisakti

    Dr. Julian Chendrasari, Sp.PA affiliated with the Department of Anatomical Pathology at the Faculty of Medicine, Universitas Trisakti, Indonesia, has made significant strides in advancing research and knowledge in the field. Dr. Chendrasari's dedication to medical research is evident through her prolific academic contributions. Further details on her works can be found on her ORCID profile: https://orcid.org/0009-0004-9205-3878. She can be reached for academic collaborations and discussions at julian@trisakti.ac.id.

  • Dyah Ayu Woro Setyaningrum, Department of Anatomical Pathology – Faculty of Medicine – Universitas Trisakti

    Dr. Dyah Ayu Woro Setyaningrum, M.Biomed is a prominent figure in the Department of Anatomical Pathology at the Faculty of Medicine, Universitas Trisakti, Indonesia. With a rich background in anatomical pathology, she has been actively involved in various research initiatives that aim to further medical science. Her scholarly works can be referenced through her ORCID profile: https://orcid.org/0000-0002-5551-1874. Academic and professional inquiries can be directed to dyahayu94@trisakti.ac.id.

  • Florinda Ilona, Department of Anatomical Pathology – Faculty of Medicine – Universitas Trisakti

    Dr. Florinda Ilona, Sp.PA a key member of the Department of Anatomical Pathology at the Faculty of Medicine, Universitas Trisakti, Indonesia, is known for her dedication to research and academic excellence. Her commitment to advancing the field of anatomical pathology is reflected in her active participation in numerous research projects and initiatives. She can be reached for academic correspondence at Florinda.ilona@trisakti.ac.id.

References

Martínez-Sánchez G. Practical aspects in ozone therapy: Study of the ozone concentration in the ozonized saline solution. Official Journal of Aepromo (Spanish Association of Medical Professionals in Ozone Therapy). 2020;10(1):55–68.

Menéndez S, Cepero J, Borrego L. Ozone therapy in cancer treatment: State of the art. Ozone Sci Eng. 2008 Nov;30(6):398–404.

Tang S, Xu B, Pang H, Xiao L, Mei Q, He X. Ozonated Water Inhibits Hepatocellular Carcinoma Invasion and Metastasis by Regulating the HMGB1/NF-κB/STAT3 Signaling Pathway. J Hepatocell Carcinoma. 2023;10:203–15.

Zeng J, Lei L, Zeng Q, Yao Y, Wu Y, Li Q, et al. Ozone therapy attenuates NF-ΚB–mediated local inflammatory response and activation of th17 cells in treatment for psoriasis. Int J Biol Sci. 2020;16(11):1833–45.

Krzyzanowski B, Searles Nielsen S, Turner JR, Racette BA. Fine Particulate Matter and Parkinson Disease Risk among Medicare Beneficiaries. Neurology. 2023 Nov 21;101(21):E2058–67.

J. Smith A, Oertle J, Warren D, Prato D. Ozone Therapy: A Critical Physiological and Diverse Clinical Evaluation with Regard to Immune Modulation, Anti-Infectious Properties, Anti-Cancer Potential, and Impact on Anti-Oxidant Enzymes. Open J Mol Integr Physiol. 2015;05(03):37–48.

Shrivastava S, Singh N, Nigam AK, Chandel SS, Shrivastava R, Kumar S. Comparative study of hematological parameters along with effect of chemotherapy and radiotherapy in different stages of breast cancer. Int J Res Med Sci. 2016 Dec 19;5(1):311.

Ur Rehman W, Maroof L, Khan MH, Israr M, Shah Q, Rehman U, et al. Effect of Cupping Therapy on Biochemical and Hematological Parameters. National Journal of Life and Health Sciences. 2023 Dec 30;2(2):76–80.

Luongo M, Brigida AL, Mascolo L, Gaudino G. Possible therapeutic effects of ozone mixture on hypoxia in tumor development. Vol. 37, Anticancer Research. International Institute of Anticancer Research; 2017. p. 425–36.

Li Y, Pu R. Ozone Therapy for Breast Cancer: An Integrative Literature Review. Vol. 23, Integrative Cancer Therapies. SAGE Publications Inc.; 2024.

Smith N, Wilson A, Gandhi J, Vatsia S, Khan S. Ozone therapy: An overview of pharmacodynamics, current research, and clinical utility. Vol. 7, Medical Gas Research. Medknow Publications; 2017. p. 212–9.

Luongo M, Brigida AL, Mascolo L, Gaudino G. Possible therapeutic effects of ozone mixture on hypoxia in tumor development. Vol. 37, Anticancer Research. International Institute of Anticancer Research; 2017. p. 425–36.

Krzyzanowski B, Searles Nielsen S, Turner JR, Racette BA. Fine Particulate Matter and Parkinson Disease Risk among Medicare Beneficiaries. Neurology. 2023 Nov 21;101(21):E2058–67.

Jomova K, Raptova R, Alomar SY, Alwasel SH, Nepovimova E, Kuca K, et al. Reactive oxygen species, toxicity, oxidative stress, and antioxidants: chronic diseases and aging. Vol. 97, Archives of Toxicology. Springer Science and Business Media Deutschland GmbH; 2023. p. 2499–574.

Sadighara P, Shahbazi R, Zirak MR, Mohamadi S, Karami L, Zomorodian N, et al. The content and dietary exposure of Malondialdehyde in industrial and traditional ice cream fats ARTICLE INFO ABSTRACT [Internet]. Vol. 8, J food safe & hyg. 2022. Available from: http://jfsh.tums.ac.ir

Jablonowski H, Santos Sousa J, Weltmann KD, Wende K, Reuter S. Quantification of the ozone and singlet delta oxygen produced in gas and liquid phases by a non-thermal atmospheric plasma with relevance for medical treatment. Sci Rep. 2018 Dec 1;8(1).

Pelinsari SM, Sarandy MM, Vilela EF, Novaes RD, Schlamb J, Gonçalves RV. Ozone Exposure Controls Oxidative Stress and the Inflammatory Process of Hepatocytes in Murine Models. Vol. 13, Antioxidants. Multidisciplinary Digital Publishing Institute (MDPI); 2024.

Sumida JM, Hayashi AM. Ozone therapy in veterinary medicine: clinical indications and techniques. Vol. 16, Acta Veterinaria Brasilica. Universidade Federal Rural do Semi-Arido; 2022. p. 294–304.

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Published

2024-09-23

Issue

Vol. 5 No. 1 (2024): The Avicenna Medical Journal

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Articles

How to Cite

The Impact of High Dose Ozone Therapy on Hematological and Biochemical Profiles : An Observational Study. (2024). The Avicenna Medical Journal, 5(1), 19-24. https://doi.org/10.15408/avicenna.v5i1.34859