Profil Fraksi Sitotoksik terhadap Sel Murine Leukemia P-388 dari Ekstrak Biji Honje (Etlingera elatior)

Alfindah Rusanti, Dede Sukandar, Tarso Rudiana, Adawiah Adawiah

Abstract


The research characterization of cytotoxic fraction against P-388 leukemia murine cells from the extract honje (Etlingera elatior) seed have been reported. This research lead to isolated and characterization of cytotoxic compounds against P-388 leukemia murine cells from the extract E. elantior seed. The extract of E. elantior seed was maserated by methanol, n-hexane, and ethyl acetate, respectively and estimated their cytotoxic activity against P-388 leukemia murine cell with 3- (4, 5-dimetiltiazol-2-yl) -2,5-difeniltetrazolium bromide (MTT) assay guided toxicity test against of shrimp Artemia salina Leach. Brine shirmp Lethality Test (BSLT) method. The active extracts will be separated by fractionation using column chromatography, radial chromatography, and for analyzing the purity of isolate will estimate by HPLC. The chemical structure of pure isolate will be identified by spectroscopies data UV Vis, FTIR, NMR and MS. The ethyl acetate extract from honje seed have cytotoxic activity by leukemia P-388 cell  with IC50 19.21 µg/mL. The compound toxic as cytotoxicagainst P-388 leukemia murine cells is flavonoid compouds their is resveratrol, lapachol, apigenin, methylated chrysin, 6,2’-dihydroxyflavanone, 3-hydroxy-3,4’-dymethoxyflavone and 4’-hydroxy-5,7-dimethoxyflavanone.

DOI: http://dx.doi.org/10.15408/jkv.v0i0.3640


Keywords


Etlingera elatior; cytotoxic; P-388 leukemia murine cell; BSLT

References


Antoro ED. 1995. Skrining fitokimia rimpang Nicolaia speciosa Horan. secara mikrokimiawi kromatografi lapis tipis,dan spektrofotmetri UV. [Skripsi]. Yogyakarta (ID): Fakultas Farmasi UGM.

Anto, Yudi. "Ragam Khasiat Dan Manfaat." Ragam Khasiat Dan Manfaat. N.p, 01 Jan. 1970. Web. 28 July 2017. http://ragamfaedah.blogspot.com.

Chan EWC, Lim YY, Omar M. 2007. Antioxidant and antibacterial activity of leaves of Etlingera species (Zingiberaceae) in Peninsular Malaysia. Food Chemistry. 104: 1586–1593.

Cho S, Kim HH, Lee MJ, Lee S, Park CS, Nam SJ. 1988. Novel cytotoxic polyprenilaterd xanthones from Garcinia gaundichaudii (Guttiferae). Journal of Tetrahedron Research. (54): 10915-10924.

Day RA, AL Underwood. 2002. Analisis Kimia Kuantitatif. Edisi Keenam. Jakarta (ID): Erlangga.

Habsah M, Lajis NH, Sukari MA, Yap YH, Kikuzaki H, Nakatani N, Ali AM. 2005. Antitumour-promoting and cytotoxic constituentss of Etlingera Elatior. Malaysian Journal of Medical Sciences. 12: 6-12.

Hidayat SS, Hutapea JR. 1991. Inventaris Tanaman Obat Indonesia. Edisi I: 440-441. Badan Penelitian dan Pengembangan Departemen Kesehatan Republik Indonesia.

Huang Y, Kuo M, Liu J, Huang S, Lin J. 1996. Inhibitions of protein kinase C and proto-oncogene expressions in NIH 3T3 cells by apigenin. Europe Journal of Cancer. 32: 146–151.

Ikawati Z. 2008. Pengantar Farmakologi Molekuler. Yogyakarta: Gajah Mada University Press

Jaafar FM, Osman CP, Ismail NH, Awang K. 2007. Analysis of essensial oils of leaves, stems, flowers and rhizomes of Etlingera elatior (JACK) R. M. SMITH. The Malaysian Journal of Analytical Sciences. 11 (1): 269-273.

Juniarti D, Osmeli, Yuhernita. 2009. Kandungan senyawa kimia, uji toksisitas (Brine Shrimp Lethality Test) dan antioksidan (1,1-diphenyl-2-pikrilhydrazyl) dari ekstrak daun saga (Abrusprecatorius L.). MAKARA SAINS. 13(1): 50-54.

Lukis PA, Ersam T. 2010. Dua Senyawa Mangostin dari ekstrak n-heksan pada kayu akar Manggis (Garcinia mangostana L.) Asal Kabupaten Nganjuk Jawa Timur. Prosiding Kimia FMIPA ITS. Surabaya: ITS Surabaya.

Maimulyati A, Prihadi AR. 2015. Cehmical composition, phytochemical and antioxidant activity from extract of Etlingera elantior flower from Indonesia. Journal of Pharmacognocy and Phytochemistry. 3(6): 233-238.

Meyer BNNR, ML Ferrigni. 1982. Brine shrimp a convinient general bioassay for active plant constituents. Journal of Plant Medical Research. 45: 31-34.

Mc Laughlin JL, Chang CJ, Smith DL. 1991. Bench-Top, Bioassay for The Discovery of Bioactive Naturals Products, An Update, Natural Product Chemistry. Amsterdam (NL): Elseiveir.

Naufalin R. 2005. Kajian Sifat Antimikroba Bunga Kecombrang (Nicolaia speciosa Horan) terhadap Berbagai Mikroba Patogen dan Perusak Pangan. [Disertasi]. Bogor (ID): Program Pascasarjana, Institut Pertanian Bogor.

Ruela-de-Sousa, Fuhler GM, Blom N, Ferreira CV, Aoyama H, Peppelenbosch MP. 2010. Cytotoxicity of Apigenin on leukemia cell lines: implications for prevention and therapy. Cell Death Dis. 1(1): 19.

Saputra K. 2000. Terapi Biologi Untuk Kanker. Surabaya (ID): Airlangga University Press.2010.

Supratman U. 2010. Elusidasi Struktur Senyawa Organik. Bandung (ID): Widya Padjajaran.

Scheuer PJ. 1994. Ciguatera and its offshoots: encounters en route to a molecular structure. Tetrahedron.50:318.

Wang IKI, Lin-Shiau SY, Lin JK. 1999. Induction of apoptosis by apigenin and related flavonoids through cytochrome c release and activation of caspase-9 and caspase-3 in leukemia HL-60 cells. European Journal of Cancer. 35(1): 1517-1525.

Creswell J, Clifford, Ollaf AR, Malcolm C. 2005. Analisis Spektrum Senyawa organik. Bandung (ID): ITB.


Full Text: PDF

DOI: 10.15408/jkv.v3i1.3640

Refbacks

  • There are currently no refbacks.


Copyright (c) 2019 Alfindah Rusanti, Dede Sukandar, Tarso Rudiana, Adawiah Adawiah

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.